Bis(ethyl acetoacetato-O1',O3)bis(2-methylpropan-1-olato)titanium

Short-term toxicity to aquatic invertebrates

Description of key information

The 48-hour EC50 (immobilisation) to Daphnia magna, > 100 mg/l based on estimated test item concentrations as per OECD 202 in accordance with GLP. The test item concentration corresponds to the measured concentrations of 29 mg/l ethyl acetoacetate and 16 mg/l 2-methylpropanol in test solutions.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The key study on acute toxicity test performed for this substance identifies that toxicity of this substance is related to the degradation products due to the rapid hydrolysis which takes place immediately after test substance is introduced to water solution. The test substance is highly hydrolytically unstable and the quantitative analysis of the test substance was not possible. Therefore, the samples were analysed on the hydrolysis product of the test substance, ethyl acetoacetate and 2 -methylpropanol. The results were expressed based on the measured concentrations of ethyl acetoacetate and 2 -methylpropanol and the equivalent test item concentrations.

Under the conditions of the OECD 202 study conducted in accordance with GLP, test substance did not induce acute immobilisation of Daphnia magna at the loading rate of 100 mg/l test item, corresponding to the measured concentrations of the degradation products, 29 mg/l of ethyl acetoacetate and 16 mg/l of 2 -methylpropanol, after 48 hours. The 48h-EC50 (immobilisation) was beyond the range tested. The NOEC was 100 mg test item/l corresponding to 29 mg/l of ethyl acetoacetate and 16 mg/l of 2 -methylpropanol in the test solutions.

Supporting study on 2 -methylpropanol reported the lowest 48-h EC50- value of 1 300 mg/l to D.magna (Elnabarawy and Welter et al. 1986). The 24-h EC50 (immobilisation) of ethyl acetoacetate to Daphnia magna is 790 to 800 mg/l based on nominal test concentrations. LC100 values varied between 1 250 to 1 136 mg/l, respectively (European Chemicals Bureau 2002).

Results of the key study do not lead to the classification of the substance as toxic to aquatic organisms in accordance with the criteria set out in Directive 67/548/EC and Regulation (EC) 1272/2008. The key study is classified as reliable without restrictions and satisfies the guideline requirements for the acute invertebrate toxicity study.