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EC number: 222-111-5 | CAS number: 3351-05-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, test procedure in accordance with OECD 406 methods, meets generally accepted scientific principles, acceptable for assessment. Not GLP compliant.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 1976
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Hazardous substance Labelling Act Sect. 191-12 (february 1965)
- Deviations:
- yes
- Remarks:
- slight modifications
- GLP compliance:
- no
Test material
- Reference substance name:
- Sodium 8-phenylamino-5-(4-(3-sulphonatophenylazo)-1-naphthylazo)naphthalenesulphonate
- EC Number:
- 222-111-5
- EC Name:
- Sodium 8-phenylamino-5-(4-(3-sulphonatophenylazo)-1-naphthylazo)naphthalenesulphonate
- Cas Number:
- 3351-05-1
- Molecular formula:
- C32H23N5O6S2.2Na
- IUPAC Name:
- disodium 8-(phenylamino)-5-[(1E)-2-{4-[(1E)-2-(3-sulfonatophenyl)diazen-1-yl]naphthalen-1-yl}diazen-1-yl]naphthalene-1-sulfonate
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- Healthy New Zealand White rabbits (Porton strain) aged 12-16 weeks with average body weights of 2.70 kgs. (males ) and 2.58 kgs (females) bred on the premises and maintained under a semi-barrier system. Six rabbits (3 males and 3 females) were acclimatised in the test area for one week prior to the start of the trial.The rabbits were caged singly in an experimental room maintained at a temperature of 20°C. (_+1°) and a relative humidityof 50-70%. Animals ware exposed to artificial light for 10 hours daily from 08.00 - 18.00 hours. A commercial irradiated diet(Styles-Oxoid) was fed ad lib. Sterile filtered water was available at all times.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: the right eye served as control
- Amount / concentration applied:
- 100 mg of the test compound were instilled into the conjunctival sac of the left eye. The eyelids were then held closed for 1 second. The right eye served as a control. After 30 seconds the compound was, as far as possible, flushed out of the eyes of three of the rabbits with approximately200 ml. of warm water.
- Duration of treatment / exposure:
- 30 seconds
- Observation period (in vivo):
- up to 16 days
- Number of animals or in vitro replicates:
- 6 (3 males and 3 females)
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: all animals
- Time point:
- other: 1 h
- Score:
- 1
- Reversibility:
- fully reversible within: 6 days
- Irritation parameter:
- iris score
- Basis:
- animal: 11,13
- Time point:
- 24 h
- Score:
- 1
- Reversibility:
- fully reversible within: 10 days
- Irritation parameter:
- conjunctivae score
- Remarks:
- Redness
- Basis:
- animal: all animals
- Time point:
- other: 1 h
- Score:
- 2
- Reversibility:
- fully reversible within: 15 days
- Irritation parameter:
- chemosis score
- Basis:
- animal: 11,15,16
- Time point:
- other: 1 h
- Score:
- 1
- Reversibility:
- fully reversible within: 10 days
- Irritation parameter:
- chemosis score
- Basis:
- animal: 13,14
- Time point:
- other: 1 h
- Score:
- 2
- Reversibility:
- fully reversible within: 10 days
- Irritation parameter:
- chemosis score
- Basis:
- animal: 14
- Time point:
- other: 1 h
- Score:
- 3
- Reversibility:
- fully reversible within: 10 days
- Irritation parameter:
- conjunctivae score
- Remarks:
- Discharge
- Basis:
- animal: all animals
- Time point:
- other: 1 h
- Score:
- 3
- Reversibility:
- fully reversible within: 6 days
- Irritation parameter:
- chemosis score
- Time point:
- 24/48/72 h
- Remarks on result:
- positive indication of irritation
- Irritation parameter:
- conjunctivae score
- Time point:
- 24/48/72 h
- Remarks on result:
- positive indication of irritation
- Irritation parameter:
- iris score
- Time point:
- 24/48/72 h
- Remarks on result:
- positive indication of irritation
- Irritation parameter:
- cornea opacity score
- Time point:
- 24/48/72 h
- Remarks on result:
- positive indication of irritation
Any other information on results incl. tables
Time |
Rabbit no. |
11 |
13 |
15 |
12 |
14 |
16 |
1 h |
Cornea Opacity |
1 |
1 |
1 |
1 |
1 |
1 |
Iris |
|
|
|
|
|
|
|
Conjunctivae Redness Chemosis Discharge |
2 1 3 |
2 2 3 |
2 1 3 |
2 3 3 |
2 2 3 |
2 1 3 |
Time |
Rabbit no. |
11 |
13 |
15 |
12 |
14 |
16 |
1 day |
Cornea Opacity |
1 |
1 |
|
1 |
1 |
1 |
Iris |
1 |
1 |
|
|
|
|
|
Conjunctivae Redness Chemosis Discharge |
2 2 2 |
2 3 3 |
2 1
|
2 3 2 |
2 2 1 |
2 1
|
Time |
Rabbit no. |
11 |
13 |
15 |
12 |
14 |
16 |
3 days |
Cornea Opacity |
1 |
1 |
|
1 |
|
1 |
Iris |
1 |
1 |
|
|
|
|
|
Conjunctivae Redness Chemosis Discharge |
3 2 1 |
3 2 2 |
1
|
3 2 2 |
2 1
|
3
|
Time |
Rabbit no. |
11 |
13 |
15 |
12 |
14 |
16 |
6 days |
Cornea Opacity |
1 |
|
|
|
|
|
Iris |
1 |
1 |
|
|
|
|
|
Conjunctivae Redness Chemosis Discharge |
2 1
|
2 2
|
1
|
2 1
|
1
|
1
|
Time |
Rabbit no. |
11 |
13 |
15 |
12 |
14 |
16 |
10 days |
Cornea Opacity |
|
|
|
|
|
|
Iris |
|
|
|
|
|
|
|
Conjunctivae Redness Chemosis Discharge |
|
1 |
|
1 |
|
|
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- irritant for the eye
- Executive summary:
The ocular reactions were scored by the method described in "Appraisal of the Safety of Chemicals in Food Drugs and Cosmetics" page 51, published by the Association of Food and Drug Officials of the U.S.A. (sea appendix I).The tested substance is considered to be irritant for the rabbits eyes.
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