Icosyl acrylate

Long-term toxicity to aquatic invertebrates

Description of key information

In a chronic toxicity test to Daphnia magna STRAUSS of the test substance Laurylacrylate 12, the overall effect threshold for effects of the test item under the test conditions was 0.250 μg/L (NOEC) and > 0.250 μg/L (LOEC) based on the geometric mean measured concentrations of the test item.

A confirmatory long-term aquatic invertebrate test with octadecyl acrylate is propsed to strengthen the read across.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.25 µg/L

Additional information

A semi-static Daphnia magna reproduction with the test item Laurylacrylat 12 was conducted at the test facility according to OECD guideline 211. Test species was Daphnia magna STRAUS. Ten daphnids, held individually, were used perconcentration, solvent control and control. Since the test item is volatile, the study was carried out in a closed system without headspace (sealed glass flasks filled up to the top with the test solutions) under semi-static conditions with a daily renewal of the test solutions. One limit concentration (1 μg/L) of the test item was prepared by dissolving the test item in DMF and adding the test item dissolved in the solvent to the dilution water. The solvent concentration was 50.0 μL/L. The geometric mean measured test item concentration is 0.250 μg/L. The measured concentrations of the test item in fresh test media (0 hours) were in the range of 0.509 μg/L to 1.66 μg/l. At the end of the respective exposure intervals (24 hours), the measured concentrations in the old media were in the range of < LOQ to 0.152 μg/L. The tested concentration was above the limit of solubility. Thus, the observed decrease may be caused by separation of undissolved test material. The regular test duration is 21 days. The test was prolonged for one day as the release of juveniles had not been synchronous. Therefore, the evaluation is performed based on the data of days 0-21 and 0-22. The Cochran-Armitage trend-test revealed that no significant trend in mortality was observed. The overall effect threshold for effects of the test item under the test conditions was 0.250 μg/L (NOEC) and > 0.250 μg/L (LOEC) based on the geometric mean measured concentrations of the test item Laurylacrylat 12.

In an supporting study with a read across substane 2-Propenoic acid, C12-14-alkyl esters (mixture of CAS no. 2156 -97 -0 and 21643 -42 -5), a semi-static Daphnia magna reproduction test was performed in closed test vessels according to OECD Guideline 211 (2008) and EU Method C.20 (2008). At this method Daphnia magna, aged lower or equal 24 h at the beginning of the test, are exposed to a range of a read across substance under defined conditions over a period of 21 days. In the definitive limit test the nominal concentration was 100 mg/L. The read across substance is poorly water soluble and volatile; therefore a saturated stock solution was prepared using a liquid-liquid saturator technique. Concentration control analysis was not performed since the analytical detection limit was above the water solubility of the test substance. However, all reasonable efforts were taken to produce a saturated solution of the test substance in test media, following the guidance in OECD 23 (2000).

The following overall effect concentrations (mg/L) were obtained based on nominal concentrations: LOEC > 100 mg/L NOEC ≥ 100 mg/L No significant effect on mortality (21 d EC0 ≥ 100 mg/L) and reproduction or any adverse effects on additional endpoints (i.e. growth, immobile young, time to first brood, or aborted eggs) were observed in any of the test treatments.