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EC number: 203-685-6 | CAS number: 109-59-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP test according to guideline. The test report from the Japanese Ministry of Health, Labour and Welfare is not available, but all the data were validated by the Japanese authorities within the OECD SIDS program Information in this record is from therefore from the SIDS dossier, a reliable secondary source, and used as supportive information.
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- Unnamed
- Year:
- 2 009
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- not specified
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-isopropoxyethanol
- EC Number:
- 203-685-6
- EC Name:
- 2-isopropoxyethanol
- Cas Number:
- 109-59-1
- Molecular formula:
- C5H12O2
- IUPAC Name:
- Propan-2-ol
- Details on test material:
- - Purity test date: >=99.50%- Manufacture: Mitsui Chemicals, INC.
- Lot/batch No.: 30321
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- E. coli WP2 uvr A
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver, induced with phenobarbital and 5,6-benzoflavone
- Test concentrations with justification for top dose:
- the dose-finding study : 0, 8.19, 20.5, 51.2, 128, 320, 800, 2000, 5000 ug/plate
the main study : 0, 313, 625, 1250, 2500, 5000 ug/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Water for injection
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Source: Wako Pure Chemical Industries, Ltd
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide
- Remarks:
- Source: Wako Pure Chemical Industries, Ltd
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 9-aminoacridine hydrochloride
- Remarks:
- Source: SIGMA-ALDRICH Corp
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- Source: Wako Pure Chemical Industries, Ltd
- Details on test system and experimental conditions:
- [METHOD OF APPLICATION]pre-incubation[DURATION]
- Preincubation period: 20 minutes
- Exposure duration: 48 hours
[plate/dose]=3 - Evaluation criteria:
- The chemicals were considered to be mutagenic when a dose-related increase in revertant colony count was observed and the number of revertant colonies per plate with the test substance was more than twice that of the negative control and when a reproducibility of test result was observed.
- Statistics:
- Not conducted
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1 |
Summary data of dose-finding study with 2-(1-methylethoxy)ethanol [non-activation method:-S9 mix] |
|||||||||||||||
|
|
|||||||||||||||
Compound |
Dose |
Revertant colonies per plate [Mean +/- S.D.] |
||||||||||||||
TA 100 |
TA 1535 |
WP2 uvrA |
TA 98 |
TA 1537 |
||||||||||||
Test substance |
0 |
113 |
121 |
115 |
11 |
11 |
13 |
25 |
26 |
23 |
23 |
25 |
26 |
7 |
5 |
7 |
[116 +/- 46] |
[12 +/- 1] |
[25 +/- 2] |
[25 +/- 2] |
[6 +/- 1] |
||||||||||||
8.19 |
105 |
103 |
115 |
9 |
7 |
11 |
26 |
20 |
22 |
19 |
23 |
20 |
7 |
9 |
7 |
|
[108 +/- 6] |
[9 +/- 2] |
[23 +/- 3] |
[21 +/- 2] |
[8 +/- 1] |
||||||||||||
20.5 |
102 |
110 |
109 |
11 |
8 |
12 |
23 |
32 |
25 |
18 |
21 |
26 |
8 |
6 |
11 |
|
[107 +/- 4] |
[10 +/- 2] |
[27 +/- 5] |
[22 +/- 4] |
[8 +/- 3] |
||||||||||||
51.2 |
101 |
112 |
104 |
6 |
5 |
8 |
31 |
26 |
25 |
19 |
21 |
16 |
8 |
8 |
10 |
|
[106 +/- 6] |
[6 +/- 2] |
[27 +/- 3] |
[19 +/- 3] |
[9 +/- 1] |
||||||||||||
128 |
127 |
106 |
102 |
12 |
7 |
7 |
22 |
30 |
31 |
18 |
24 |
21 |
7 |
8 |
8 |
|
[112 +/- 13] |
[9 +/- 3] |
[28 +/- 5] |
[21 +/- 3] |
[8 +/- 1] |
||||||||||||
320 |
119 |
116 |
129 |
12 |
11 |
6 |
26 |
28 |
27 |
28 |
26 |
23 |
5 |
8 |
5 |
|
[121 +/- 7] |
[10 +/- 3] |
[27 +/- 1] |
[26 +/- 3] |
[6 +/- 2] |
||||||||||||
800 |
101 |
101 |
92 |
11 |
10 |
12 |
23 |
30 |
23 |
28 |
25 |
25 |
8 |
10 |
7 |
|
[98 +/- 5] |
[11 +/- 1] |
[25 +/- 4] |
[26 +/- 2] |
[8 +/- 2] |
||||||||||||
2000 |
103 |
100 |
118 |
12 |
7 |
9 |
26 |
24 |
20 |
28 |
27 |
25 |
6 |
6 |
8 |
|
[107 +/- 10] |
[9 +/- 3] |
[23 +/- 3] |
[27 +/- 2] |
[7 +/- 1] |
||||||||||||
5000 |
104 |
96 |
98 |
15 |
13 |
19 |
26 |
27 |
18 |
30 |
30 |
23 |
11 |
9 |
8 |
|
[99 +/- 4] |
[16 +/- 3] |
[24 +/- 5] |
[28 +/- 4] |
[9 +/- 2] |
||||||||||||
Positive control |
|
589 |
631 |
558a) |
531 |
543 |
515b) |
166 |
187 |
149a) |
703 |
680 |
726c) |
314 |
255 |
285d) |
|
|
[593 +/- 37] |
[530 +/- 14] |
[167 +/- 19] |
[703 +/- 23] |
[285 +/- 30] |
||||||||||
|
||||||||||||||||
a) AF-2:2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide, 0.01ug/plate; b) NaN3:Sodium azide, 0.5ug/plate; c) AF-2,0.1ug/plate; d) 9-AA; 9-Aminoacridine hydrochloride, 80ug/plate |
Table 2 |
Summary data of dose-finding study with 2-(1-methylethoxy)ethanol [activation method:+S9 mix] |
|||||||||||||||
|
|
|||||||||||||||
Compound |
Dose |
Revertant colonies per plate [Mean +/- S.D.] |
||||||||||||||
TA 100 |
TA 1535 |
WP2 uvrA |
TA 98 |
TA 1537 |
||||||||||||
Test substance |
0 |
114 |
94 |
101 |
10 |
13 |
10 |
29 |
31 |
28 |
33 |
35 |
33 |
19 |
18 |
17 |
[103 +/- 10] |
[11 +/- 2] |
[29 +/- 2] |
[34 +/- 1] |
[18 +/- 1] |
||||||||||||
8.19 |
114 |
136 |
119 |
13 |
12 |
9 |
33 |
32 |
34 |
34 |
29 |
34 |
19 |
13 |
18 |
|
[123 +/- 12] |
[11 +/- 2] |
[33 +/- 1] |
[32 +/- 3] |
[17 +/- 3] |
||||||||||||
20.5 |
104 |
117 |
95 |
14 |
14 |
17 |
26 |
29 |
35 |
32 |
24 |
31 |
15 |
20 |
20 |
|
[105 +/- 11] |
[15 +/- 2] |
[30 +/- 5] |
[29 +/- 4] |
[18 +/- 3] |
||||||||||||
51.2 |
116 |
109 |
110 |
12 |
7 |
13 |
30 |
38 |
38 |
31 |
32 |
38 |
18 |
19 |
19 |
|
[112 +/- 4] |
[11 +/- 3] |
[35 +/- 5] |
[34 +/- 4] |
[19 +/- 1] |
||||||||||||
128 |
105 |
111 |
130 |
11 |
13 |
14 |
32 |
34 |
30 |
33 |
31 |
31 |
24 |
22 |
22 |
|
[115 +/- 13] |
[13 +/- 2] |
[32 +/- 2] |
[32 +/- 1] |
[23 +/- 1] |
||||||||||||
320 |
105 |
113 |
108 |
9 |
12 |
10 |
36 |
30 |
27 |
34 |
30 |
28 |
15 |
17 |
14 |
|
[109 +/- 4] |
[10 +/- 2] |
[31 +/- 5] |
[31 +/- 3] |
[15 +/- 2] |
||||||||||||
800 |
94 |
101 |
111 |
9 |
14 |
9 |
29 |
34 |
32 |
31 |
34 |
26 |
23 |
15 |
21 |
|
[102 +/- 9] |
[11 +/- 3] |
[32 +/- 3] |
[30 +/- 4] |
[20 +/- 4] |
||||||||||||
2000 |
125 |
114 |
123 |
15 |
11 |
11 |
31 |
37 |
33 |
27 |
27 |
26 |
17 |
21 |
16 |
|
[121 +/- 6] |
[12 +/- 2] |
[34 +/- 3] |
[27 +/- 1] |
[18 +/- 3] |
||||||||||||
5000 |
98 |
108 |
109 |
14 |
11 |
13 |
31 |
33 |
34 |
27 |
36 |
34 |
16 |
17 |
18 |
|
[105 +/- 6] |
[13 +/- 2] |
[33 +/- 2] |
[32 +/- 5] |
[17 +/- 1] |
||||||||||||
Positive control |
|
539 |
585 |
596a) |
282 |
336 |
304b) |
712 |
623 |
657c) |
513 |
543 |
480d) |
137 |
112 |
133b) |
|
|
[573 +/- 30] |
[307 +/- 27] |
[664 +/- 45] |
[512 +/- 32] |
[127 +/- 13] |
||||||||||
|
||||||||||||||||
a)2-AA:2-Aminoanthracene, 1ug/plate; b)2-AA, 2ug/plate; c)2-AA, 10ug/plate; d)2-AA, 0.5ug/plate |
Table 3 |
Result of the bacterial reversion test of 2-(1-methylethoxy)ethanol [non-activation method:-S9 mix] |
|||||||||||||||
|
|
|||||||||||||||
Compound |
Dose |
Revertant colonies per plate [Mean +/- S.D.] |
||||||||||||||
TA 100 |
TA 1535 |
WP2 uvrA |
TA 98 |
TA 1537 |
||||||||||||
Test substance |
0 |
122 |
129 |
117 |
12 |
12 |
13 |
37 |
32 |
33 |
23 |
22 |
26 |
8 |
11 |
9 |
[123 +/- 6] |
[12 +/- 1] |
[34 +/- 3] |
[24 +/- 2] |
[9 +/- 2] |
||||||||||||
313 |
131 |
113 |
134 |
16 |
12 |
13 |
35 |
32 |
31 |
18 |
15 |
15 |
6 |
10 |
10 |
|
[126 +/- 11] |
[14 +/- 2] |
[33 +/- 2] |
[16 +/- 2] |
[9 +/- 2] |
||||||||||||
625 |
109 |
116 |
130 |
8 |
10 |
10 |
31 |
30 |
27 |
28 |
25 |
24 |
14 |
15 |
11 |
|
[118 +/- 11] |
[9 +/- 1] |
[29 +/- 2] |
[26 +/- 2] |
[13 +/- 2] |
||||||||||||
1250 |
125 |
127 |
141 |
11 |
14 |
10 |
32 |
31 |
33 |
31 |
29 |
34 |
13 |
13 |
10 |
|
[131 +/- 9] |
[12 +/- 2] |
[32 +/- 1] |
[31 +/- 3] |
[12 +/- 2] |
||||||||||||
2500 |
120 |
128 |
122 |
14 |
11 |
15 |
29 |
33 |
33 |
28 |
28 |
27 |
8 |
12 |
11 |
|
[123 +/- 4] |
[13 +/- 2] |
[32 +/- 2] |
[28 +/- 1] |
[10 +/- 2] |
||||||||||||
5000 |
150 |
132 |
145 |
16 |
11 |
17 |
28 |
34 |
32 |
23 |
25 |
30 |
14 |
11 |
11 |
|
[142 +/- 9] |
[15 +/- 3] |
[31 +/- 3] |
[26 +/- 4] |
[12 +/- 2] |
||||||||||||
Positive control |
|
572 |
615 |
531a) |
550 |
553 |
555b) |
177 |
165 |
180a) |
666 |
633 |
729c) |
330 |
382 |
354d) |
|
|
[573 +/- 42] |
[553 +/- 3] |
[174 +/- 8] |
[676 +/- 49] |
[355 +/- 26] |
||||||||||
|
||||||||||||||||
a)AF-2:2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide, 0.01ug/plate; b)NaN3:Sodium azide, 0.5ug/plate; c)AF-2, 0.1ug/plate; d)9-AA: 9-Aminoacridine hydrochloride, 80 ug/plate |
Table 4 |
Result of the bacterial reversion test of 2-(1-methylethoxy)ethanol [activation method:+S9 mix] |
|||||||||||||||
|
|
|||||||||||||||
Compound |
Dose |
Revertant colonies per plate [Mean +/- S.D.] |
||||||||||||||
TA 100 |
TA 1535 |
WP2 uvrA |
TA 98 |
TA 1537 |
||||||||||||
Test substance |
0 |
113 |
129 |
124 |
13 |
10 |
15 |
31 |
37 |
33 |
26 |
27 |
31 |
17 |
19 |
16 |
[122 +/- 8] |
[13 +/- 3] |
[34 +/- 3] |
[28 +/- 3] |
[17 +/- 2] |
||||||||||||
313 |
124 |
120 |
130 |
10 |
9 |
10 |
37 |
31 |
30 |
26 |
29 |
25 |
18 |
15 |
13 |
|
[125 +/- 5] |
[10 +/- 1] |
[33 +/- 4] |
[27 +/- 2] |
[15 +/- 3] |
||||||||||||
625 |
118 |
122 |
135 |
6 |
8 |
8 |
28 |
37 |
30 |
26 |
27 |
20 |
20 |
20 |
23 |
|
[125 +/- 9] |
[7 +/- 1] |
[32 +/- 5] |
[24 +/- 4] |
[21 +/- 2] |
||||||||||||
1250 |
137 |
131 |
128 |
13 |
10 |
8 |
38 |
37 |
37 |
22 |
27 |
24 |
18 |
21 |
24 |
|
[132 +/- 5] |
[10 +/- 3] |
[37 +/- 1] |
[24 +/- 3] |
[21 +/- 3] |
||||||||||||
2500 |
120 |
125 |
134 |
17 |
11 |
14 |
44 |
47 |
46 |
27 |
21 |
25 |
17 |
19 |
24 |
|
[126 +/- 7] |
[14 +/- 3] |
[46 +/- 2] |
[24 +/- 3] |
[20 +/- 4] |
||||||||||||
5000 |
121 |
122 |
130 |
9 |
9 |
9 |
35 |
42 |
41 |
31 |
28 |
29 |
15 |
16 |
14 |
|
[124 +/- 5] |
[9 +/- 0] |
[39 +/- 4] |
[29 +/- 2] |
[15 +/- 1] |
||||||||||||
Positive control |
|
836 |
785 |
822a) |
257 |
264 |
300b) |
693 |
667 |
668c) |
425 |
473 |
408d) |
138 |
154 |
156b) |
|
|
[814 +/- 26] |
[274 +/- 23] |
[676 +/- 15] |
[435 +/- 34] |
[149 +/- 10] |
||||||||||
|
||||||||||||||||
a)2-AA:2-Aminoanthracene, 1ug/plate; b)2-AA, 2ug/plate; c)2-AA, 10ug/plate; d)2-AA, 0.5ug/plate |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative without metabolic activation
negative with metabolic activation
The test substance was not mutagenic under the conditions of this study. - Executive summary:
The study was conducted under guideline and in compliance with GLP. The mutagenicity of 2-isopropoxyethanol was examined in four Salmonella typhimurium strains, TA98, TA100, TA1535 and TA1537, and in an Escherichia coli strain, WP2uvrA, at concentrations up to 5,000 μg/plate with or without an exogenous metabolic activation system. In this study, the substance did not show any mutagenic activity in any bacterial strain with or without metabolic activation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.