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EC number: 201-933-8 | CAS number: 89-72-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28-jul-2009 to 19-aug-2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study has been performed according to OECD and/or EC guidelines and according to GLP principles.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- The batch of 2 -sec-butylphenol tested was a clear colourless liquid with a purity of 99.6%.
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all test concentrations and the control
- Sampling method: volume, 2 ml from the approximate centre of the test vessels at t=0 h and t=48 h
- Sample storage conditions before analysis: Samples were stored in a freezer until analysis - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Preparation of test solutions started with the highest concentration of 100 mg/l. No special treatment other than a short period of magnetic stirring (15 minutes) was applied to completely dissolve this concentration in test medium. The lower test concentrations were prepared by subsequent dilution. The final test solutions were all clear and colourless. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: In-house laboratory culture
- Age at study initiation: < 24 hours
- Method of breeding: With newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 5 litres of medium in an all-glass culture vessel.
- Feeding during test: No - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 180 mg/l expressed as CaCO3
- Test temperature:
- Between 19.1 and 20.0°C
- pH:
- At t=0 h: 7.6
At t=48 h: 7.9 - Dissolved oxygen:
- At t=0 h: 8.9-9.0
At t=48 h: 9.0 - Nominal and measured concentrations:
- Combined limit test/Range-finding:
Nominal concentrations: 0.1, 1, 10 and 100 mg/l
Measured concentration of 1 mg/l:
At t=0 h: 98 % of nominal
At t=48 h: 97 % of initial
Final test
Nominal concentrations: 1, 1.8, 3.2, 5.6 and 10 mg/l
Measured concentrations:
At t=0 h: 101 to 104 % of nominal
At t=48 h: 97 to 102 % of initial - Details on test conditions:
- - Test vessel: 100 ml, all glass
- Type (delete if not applicable): open - ~80 ml,
- Material, size, headspace, fill volume: glass, 100 ml, normal headspace
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium
- Culture medium different from test medium: yes, for culturing M7 medium is used
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 daily
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Test concentrations: 1, 1.8, 3.2, 5.6 and 10 mg/l - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (K2Cr2O7)
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.7 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence interval between 3.4 and 4.3 mg/l
- Details on results:
- - Behavioural abnormalities: no
- Mortality of control: no
- Other adverse effects control: no - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50/LC50:
The 24h-EC50 was 0.84 mg/l with a 95% confidence interval between 0.78 and 0.94 mg/l.
The 48h-EC50 was 0.75 mg/l with a 95% confidence interval between 0.56 and 1.0 mg/l. - Validity criteria fulfilled:
- yes
- Conclusions:
- 2-sec-butylphenol did not induce acute immobilisation of Daphnia magna at 1.8 mg/l after 48 hours of exposure (NOEC).
The 48h-EC50 was 3.7 mg/l based on analytically confirmed nominal exposure concentrations (95% confidence interval between 3.4 and 4.3 mg/l). - Executive summary:
A study was perform to assess the acute toxicity in Daphnia magna of the test material 2 -sec-butylphenol. The method followed the OECD Guidelines No. 202, 2004. In addition, the procedures were designed to meet the test methods of the Commission regulation (EC) No 440/2008, Part C.2, 2008 and the ISO International Standard 6341, 1996.
The batch of 2 -sec-butylphenol tested was a clear colourless liquid with a purity of 99.6% and completely soluble in test medium at the concentrations tested.
Preparation of test solutions started with the highest concentration of 100 mg/l. No special treatment other than a short period of magnetic stirring (15 minutes) was applied to completely dissolve this concentration in test medium. The lower test concentrations were prepared by subsequent dilution.
A final test was performed based on the results of a preceding combined limit/range-finding test. Twenty daphnia per test group (4 vessels, 5 per vessel) were exposed to a control and nominal 2 -sec-butylphenol concentrations of 1.0, 1.8, 3.2, 5.6 and 10 mg/l. Samples for analytical confirmation of actual exposure concentrations were taken at the start and at the end of the 48 -hour test period.
Analyses showed that the measured concentrations were in agreement with nominal and stable during the 48 -hour test period (100 -104%).
The study met the acceptability criteria prescribed by the protocol and was considered valid.
2 -sec-butylphenol did not induce acute immobilisation of Daphnia magna at 1.8 mg/l after 48 hours of exposure (NOEC).
The 48 -EC50 was 3.7 mg/l based on analytically confirmed nominal exposure concentrations (95% confidence interval between 3.4 and 4.3 mg/l).
Reference
Description of key information
2 -sec-butylphenol did not induce acute immobilisation of Daphnia magna at 1.8 mg/l after 48 hours of exposure (NOEC).
The 48 -EC50 was 3.7 mg/l based on analytically confirmed nominal exposure concentrations (95% confidence interval between 3.4 and 4.3 mg/l).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 3.7 mg/L
Additional information
A study was perform to assess the acute toxicity in Daphnia magna of the test material 2 -sec-butylphenol. The method followed the OECD Guidelines No. 202, 2004. In addition, the procedures were designed to meet the test methods of the Commission regulation (EC) No 440/2008, Part C.2, 2008 and the ISO International Standard 6341, 1996.
The batch of 2 -sec-butylphenol tested was a clear colourless liquid with a purity of 99.6% and completely soluble in test medium at the concentrations tested.
Preparation of test solutions started with the highest concentration of 100 mg/l. No special treatment other than a short period of magnetic stirring (15 minutes) was applied to completely dissolve this concentration in test medium. The lower test concentrations were prepared by subsequent dilution.
A final test was performed based on the results of a preceding combined limit/range-finding test. Twenty daphnia per test group (4 vessels, 5 per vessel) were exposed to a control and nominal 2 -sec-butylphenol concentrations of 1.0, 1.8, 3.2, 5.6 and 10 mg/l. Samples for analytical confirmation of actual exposure concentrations were taken at the start and at the end of the 48 -hour test period.
Analyses showed that the measured concentrations were in agreement with nominal and stable during the 48 -hour test period (100 -104%).
The study met the acceptability criteria prescribed by the protocol and was considered valid.
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