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EC number: 500-240-0 | CAS number: 68958-77-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From 26 May, 2010 to 27 May, 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- KL2 due to RA
- Justification for type of information:
- Refer to section 13 of IUCLID for details on the read-across justification. The study with the read across substance is considered sufficient to fulfil the information requirements as further explained in the read across justification.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.6800 (Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- Incubation period: 3 h
- Vehicle:
- no
- Details on test solutions:
- Test concentrations: 10, 32, 100, 320 and 1000 mg test substance/L (nominal)
Two controls (tap water, synthetic sewage and inoculum, but without addition of the test substance) were tested in parallel. - Test organisms (species):
- activated sludge
- Details on inoculum:
- Activated sludge, microorganisms from a domestic waste water treatment plant was supplied by the municipal sewage treatment plant Darmstadt, Germany.
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- Controlled environment room
- Test temperature:
- 20 - 22°C during dissolution phase (24 h)
19°C during pre-incubation (3 h)
19 - 20°C during evaluation period - Dissolved oxygen:
- At least 7.8 mg O2/L
- Nominal and measured concentrations:
- Nominal concentrations: 10, 32, 100, 320 and 1000 mg test substance/L
- Details on test conditions:
- For the measurement of the respiration rate a well-mixed sample of each test medium was poured into a Karlsruher flask after exactly 3 h incubation time, and was not further aerated. The oxygen concentration was measured with an oxygen electrode, and was recorded for about 10 min. During measurement, the samples were continuously stirred on a magnetic stirrer. The oxygen consumption (in mg O2 L-1 min-1) was determined from the most linear part of the respiration curve in the range between approx 6.5 - 2.5 mg O2/L. In case of a low oxygen consumption the values over a period of 10 min were used.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5 Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 64.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- In comparison to the inoculum controls the respiration rate of the activated sludge was slightly inhibited by 4.8% and 16.7% at the two lowest nominal concentrations of 10 and 32 mg/L, respectively. At the next higher nominal concentration of 100 mg/L, the respiration rate was moderately inhibited by 26.2%, respectively, and reached a plateau at 320 mg/L and the highest nominal test concentration of 1000 mg/L with an inhibition rate of 40.5%. Test substance concentrations exceeding 1000 mg/L were not tested. Based on measured inhibition rates, no 3-h EC50 could be quantified because up to the highest nominal test concentration of 1000 mg/L less than 50% inhibition was noted after 3 h incubation. Nevertheless, the 3 h EC50 is clearly higher than 1000 mg/L under the present test conditions. The 3 h EC20 was calculated by linear regression analysis using the inhibition rates of the 320, 100 and 32 mg/L test concentrations, and was calculated to be 64.6 mg/L nominal.
- Results with reference substance (positive control):
- The following nominal concentrations of the positive control 3,5-dichlorophenol were tested on the same activated sludge and under identical conditions as the test substance: 32, 10 and 3.2 mg/L. In comparison to the controls the respiration rate of the activated sludge was moderately inhibited by 35.7% at the lowest nominal concentration of 3.2 mg/L. At the nominal concentrations of 10 and 32 mg test substance/L, the respiration rate was inhibited by 42.9 and 81.0%, respectively. The 3 h EC50 of 3,5-Dichlorophenol was calculated to be 9.1 mg/L. Due to mathematical reasons, no 95 % confidence limits could be determined.
- Validity criteria fulfilled:
- yes
- Remarks:
- The respiration rates of the two controls did not differ by more than 15%. The coefficient of variation was 1.7%.
- Conclusions:
- Based on the results of the read across study, the 3 h EC20 and EC50 values of the test substance are considered to be 64.6 and >1,000 mg/L, respectively.
- Executive summary:
A study was performed to assess the inhibitory effect of the read across substance BADGEDA, on the oxygen consumption rate of aerobic microorganisms (activated sludge) according to OECD Guideline 209, EU Method C.11 and EPA OPPTS 850.6800, in compliance with GLP. The read across substance is insoluble in water. Therefore, it was directly weighed into the test vessels and stirred for about 24 h before using in the experiment. In comparison to the inoculum controls, the respiration rate of the activated sludge was slightly inhibited by 4.8% at the lowest nominal test concentrations of 10 mg/L. At the next higher concentrations of nominal 32 mg/L and 100 mg/L, inhibition was 16.7 and 26.2%, respectively. At concentrations of 320 and 1,000 mg/L, respiration rate was moderately inhibited by 40.5% at a plateau. No dose related response was found. Concentrations exceeding 1,000 mg/L nominal were not tested. Under the conditions of the study, the 3 h EC20 was calculated to be 64.6 mg/L while the 3 h EC50 was considered to exceed the highest nominal test concentration of 1000 mg/L (Feil, 2010). Based on the results of the read across study, similar EC20 and EC50 values can be considered for the test substance.
Reference
Description of key information
Based on the results of the read across study with BADGEDA, the 3 h EC20 and EC50 values of the test substance are considered to be 64.6 and >1,000 mg/L (nominal) respectively.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
A study was performed to assess the inhibitory effect of the read across substance '4,4’-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, esters with acrylic acid (BADGEDA)', on the oxygen consumption rate of aerobic microorganisms (activated sludge) according to OECD Guideline 209, EU Method C.11 and EPA OPPTS 850.6800, in compliance with GLP. The read across substance is insoluble in water. Therefore, it was directly weighed into the test vessels and stirred for about 24 h before using in the experiment. In comparison to the inoculum controls, the respiration rate of the activated sludge was slightly inhibited by 4.8% at the lowest nominal test concentrations of 10 mg/L. At the next higher concentrations of nominal 32 mg/L and 100 mg/L, inhibition was 16.7 and 26.2%, respectively. At concentrations of 320 and 1,000 mg/L, respiration rate was moderately inhibited by 40.5% at a plateau. No dose related response was found. Concentrations exceeding 1,000 mg/L nominal were not tested. Under the conditions of the study, the 3 h EC20 was calculated to be 64.6 mg/L while the 3 h EC50 was considered to exceed the highest nominal test concentration of 1000 mg/L (Feil, 2010). Based on the results of the read across study, similar EC20 and EC50 values can be considered for the test substance.
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