3-chloropropionic acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Test method was similar to OECD guideline 471, but there is no data on positive controls. No data on GLP.

Data source

Reference

Reference Type:

secondary source

Title:

Mutagenicity test data of existing chemical substances based on the toxicity investigation system of the industrial safety and health law.

Author:

Japan Chemical Industry Ecology-Toxicology & Information Center (JETOC)

Year:

2005

Bibliographic source:

Japan Chemical Industry Ecology-Toxicology & Information Center (JETOC). Mutagenicity test data of existing chemical substances based on the toxicity investigation system of the industrial safety and health law. Supplement 3, 2005.

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

not specified

Remarks:

(No data on positive controls)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3-chloropropionic acid

EC Number:

203-534-4

EC Name:

3-chloropropionic acid

Cas Number:

107-94-8

Molecular formula:

C3H5ClO2

IUPAC Name:

3-chloropropanoic acid

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A pKM 101

Metabolic activation:

with and without

Metabolic activation system:

Rat, Liver, S9, Sodium phenobarbital and 5,6-benzoflavone (10%)

Test concentrations with justification for top dose:

0, 1.22, 4.88, 19.5, 78.1, 313, 1250, 5000 µg/plate

Vehicle / solvent:

Water

Controls

Negative solvent / vehicle controls:

yes

Positive controls:

not specified

Details on test system and experimental conditions:

Method: Preincubation

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium, other: TA 100 and TA 1535

Metabolic activation:

with and without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Positive controls validity:

not specified

Test results 2

Species / strain:

E. coli WP2 uvr A pKM 101

Metabolic activation:

with and without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Positive controls validity:

not specified

Test results 3

Species / strain:

S. typhimurium, other: TA 98 and TA 1537

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Positive controls validity:

not specified

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Test substance was mutagenic on Salmonella typhimurium TA 100 and TA 1535, and on E. coli WP2 uvrA pKM 101, with and without metabolic activation. It was non-mutagenic on Salmonella typhimurium TA 98 and TA 1537, with and without metabolic activation.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
ambiguous

Test substance was mutagenic on Salmonella typhimurium TA 100 and TA 1535, and on E. coli WP2 uvrA pKM 101, with and without metabolic activation. It was non-mutagenic on Salmonella typhimurium TA 98 and TA 1537, with and without metabolic activation.

Executive summary:

3-chloropropanoic acid was tested for mutagenicity in the strains TA 100, TA 1535, TA 1537, and TA 98 of Salmonella typhimurium and in E. coli WP2 uvrA pKM 101. The mutagenicity studies were conducted in the absence and in the presence of a metabolizing system derived from rat liver homogenate. The tested concentrations were: 0, 1.22, 4.88, 19.5, 78.1, 313, 1250, and 5000 µg/plate.

Test substance was mutagenic on Salmonella typhimurium TA 100 and TA 1535, and on E. coli WP2 uvr A pKM 101, with and without metabolic activation. It was non-mutagenic on Salmonella typhimurium TA 98 and TA 1537, with and without metabolic activation.