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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 939-725-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Referenced sources in an international report.
Data source
Reference
- Reference Type:
- review article or handbook
- Title:
- Dimethylformamide, SIDS Initial Assessment Report For SIAM 13
- Author:
- OECD
- Year:
- 2 001
- Bibliographic source:
- UNEP PUBLICATIONS
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- standard plate incorporation assay, (Ames, 1975)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- N,N-dimethylformamide
- EC Number:
- 200-679-5
- EC Name:
- N,N-dimethylformamide
- Cas Number:
- 68-12-2
- Molecular formula:
- C3H7NO
- IUPAC Name:
- N,N-dimethylformamide
- Test material form:
- not specified
Constituent 1
Method
- Target gene:
- No data
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 mix prepared from liver homogenate of Aroclor pretreated male Sprague-Dawley rats.
- Test concentrations with justification for top dose:
- 9400, 24000, 47000, 94000, 190000, 470000 µg/plate
Controls
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: With metabolic activation: 2-Aminoanthracene: 5, 10 and 100 μg/plate. Without metabolic activation: N-Methyl-N'-nitro-N-nitrosoguanidine: 2 μg/plate (TA1535, TA100); 9-Aminoacridine: 50 μg/plate (TA1537); 2-Nitrofluorene: 25 μg/plate (TA1538, TA98)
- Details on test system and experimental conditions:
- Plates are incubated at 37°C for 48 hours. Cytotoxicity of the test sample as measured in strain TA1535 is the basis for selecting concentrations to be used in the present test. Concentrations of test sample that give less than 50% of control survival are normally not selected for the assay.
- Evaluation criteria:
- A chemical was classified as non-mutagenic if the reversion frequency was less than 2 times the spontaneous frequency. No statistical analysis was performed.
However, in the present assay 470000 μg/plate was chosen because this experiment was done to aid in evaluating a recent Utah Biological Testing Service report, that concluded that DMF is mutagenic in 4 of the Ames strains of Salmonella typhimurium. 470000 μg/plate led to toxicity as indicated by sparse background lawn.
Results and discussion
Test results
- Species / strain:
- other: TA98, TA100, TA1535, TA1537, and TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: Cytotoxicity occurred at 190000 and 470000 μg/plate without metabolic activation and at 470000 μg/plate with metabolic activation
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
In the Ames test, DMF was not found mutagenic even at concentrations that are cytotoxic.
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