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EC number: 910-853-9 | CAS number: 8011-63-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with Good laboratory Practice and internationally accepted guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- calcium tetracopper(2+) dioxocopperbis(olate) hexahydroxide sulfate
- EC Number:
- 910-853-9
- Cas Number:
- 8011-63-0
- Molecular formula:
- Ca3Cu4H6O22S4.nH2O where n = 1 to 6
- IUPAC Name:
- calcium tetracopper(2+) dioxocopperbis(olate) hexahydroxide sulfate
- Details on test material:
- - Name of test material (as cited in study report): Bordeaux Mixture
- Purity test date: Not stated
- Lot/batch No.: 3/0371
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Rats were housed in groups of up to five by sex, acclimatised, and fasted overnight prior to dosing. Food was returned four hours after dosing. Body weight at study initiation was 128 to 249 grams.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Remarks:
- 0.5%
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 10 ml/kg on day 1. - Doses:
- Dose levels (based on a range-finding study) were 1600, 2000, 2400 and 2800 mg/kg bw.
- No. of animals per sex per dose:
- Groups of five males and five females.
- Control animals:
- no
- Details on study design:
- Animals were observed frequently on the day of dosing and then once daily for the 14 day post-dosing period. Animals were weighed prior to administration and after 7 days (on Day 8) and after 14 days (on Day 15) or at death. Decedents and animals surviving to 14 days were subject to gross necropsy.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 2 187 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 1 843 - 2 499
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 2 433 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 2 052 - 3 344
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 302 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 2 098 - 2 516
- Mortality:
- There were no mortalities at 1600 mg/kg bw. At higher doses, the frequency of mortality increased and all males and four females died (or were killed in extremis) following 2800 mg/kg bw. The deaths occurred between Day 2 and Day 13. A summary of mortalities is shown in Table 1.
- Clinical signs:
- other: There was a variety of clinical signs recorded including piloerection, soiled coat, reduced activity, red nasal and ocular discharge, ataxia, pale and hunched appearance, swollen abdomen, tail lesions, subdued behaviour, diarrhoea, dark eyes, laboured bre
- Gross pathology:
- No gross findings were recorded in surviving animals except for dark red foci on the lungs in one male dosed at 1600 mg/kg bw. Findings in animals that died during the study included reddening of the glandular mucosa of the stomach, liquid retention in the stomach and intestines, green coloured kidneys, small seminal vesicles and light coloured foci on the caecum.
- Other findings:
- None.
Any other information on results incl. tables
Table 1. Summary of Mortalities
Dose |
Males |
Females |
||
Mortality |
Time of death |
Mortality |
Time of death |
|
1600 |
0/5 |
- |
0/5 |
- |
2000 |
1/5 |
Day 2 |
1/5 |
Day 3 |
2400 |
4/5 |
Day 9 (2); Day 10; Day 13 |
2/5 |
Day 4; Day 7 |
2800 |
5/5 |
Day 3; Day 5; |
4/5 |
Day 5 (2); |
Figures in parenthesis are the number which died on the day specified if more than one. |
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The acute oral LD50 of Bordeaux Mixture to the rat was 2187 mg/kg bw for males, 2433 mg/kg bw for females and 2302 mg/kg bw for the sexes combined.
Classification according to Directive 67/548/EEC: Not classified.
Classification according to CLP/GHS: Not classified. - Executive summary:
A GLP-compliant acute oral toxicity study was conducted in accordance with the requirements of EU Guideline B.1 and OECD 401. Bordeaux Mixture was administered as a solution in 0.5% carboxymethyl cellulose. Groups of five male and five female Sprague-Dawley rats weighing 128 to 249 g were used. The rats were housed in groups of up to five by sex, acclimatised, and fasted overnight prior to dosing. Food was returned four hours after dosing. Dose levels (based on a range-finding study) of 1600, 2000, 2400 and 2800 mg/kg bw were administered by single oral administration by gavage in 10 mL/kg on Day 1. Animals were observed frequently on the day of dosing and then once daily for the 14‑day post-dosing period. Animals were weighed prior to administration and after 7 days (on Day 8) and after 14 days (on Day 15) or at death. Decedents and animals surviving to 14 days were subject to gross necropsy.
There were no mortalities at 1600 mg/kg bw. At higher doses, the frequency of mortality increased and all males and four females died (or were killed in extremis) following 2800 mg/kg bw. The deaths occurred between Day 2 and Day 13. There was a variety of clinical signs recorded including piloerection, soiled coat, reduced activity, red nasal and ocular discharge, ataxia, pale and hunched appearance, swollen abdomen, tail lesions, subdued behaviour, diarrhoea, dark eyes, laboured breathing, prostration, hypothermia, alopecia and emaciation. Most symptoms occurred between Day 2 and 8 though some effects persisted up to Day 15. Surviving animals showed acceptable weight gain during the study. No gross findings were recorded in surviving animals except for dark red foci on the lungs in one male dosed at 1600 mg/kg bw. Findings in animals that died during the study included reddening of the glandular mucosa of the stomach, liquid retention in the stomach and intestines, green coloured kidneys, small seminal vesicles and light coloured foci on the caecum.
The acute oral LD50 (calculated by probit analysis) of Bordeaux Mixture to the rat was 2187 mg/kg bw for males (with 95% confidence limits of 1843 to 2499 mg/kg bw), 2433 mg/kg bw for females (with 95% confidence limits of 2052 to 3344 mg/kg bw) and 2302 mg/kg bw for the sexes combined (with 95% confidence limits of 2098 to 2516 mg/kg bw). On this basis, Bordeaux Mixture is not classified.
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