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EC number: 216-088-0 | CAS number: 1493-27-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well conducted, following standard guidelines, but not GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 1-fluoro-2-nitrobenzene
- EC Number:
- 216-088-0
- EC Name:
- 1-fluoro-2-nitrobenzene
- Cas Number:
- 1493-27-2
- Molecular formula:
- C6H4FNO2
- IUPAC Name:
- 1-fluoro-2-nitrobenzene
- Reference substance name:
- 1-fluoro-2-nirobenzene
- IUPAC Name:
- 1-fluoro-2-nirobenzene
- Details on test material:
- - Name of test material (as cited in study report): 2- Fluoronitrobenzene
- Physical state: liquid
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Animals were supplied by Bantin and Kingman Ltd., Hull, yorks.
- Age at study initiation: no data
- Weight at study initiation: 160 +/- 20 g
- Fasting period before study: yes, animals were fasted overnight prior to administration of the test material.
- Housing: The animals were segregated according to sex and housed in grid-floor cages.
- Diet: Standard pelleted laboratory animal diet (41B from E. Dixon and Son (Ware) Ltd., Herts) was provided at all times with the exception that the animals were fasted overnight prior to administration of the test material.
- Water: ad libitum
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/-2
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod: 12 hrs dark / 12 hrs light
IN-LIFE DATES: no data
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- maize oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 20 % v/v (range finding assay) 10 % v/v ( final assay) - Doses:
- Range finding assay: 0.8, 0.4, 0.2 and 0.1 ml/kg (corresponding to 1070, 535, 267.5 and 133.75 mg/kg bw)
Final assay: 0.40, 0.28, 0.20 and 0.1 ml/kg (corresponding to 535, 374.5, 267.5 and 187.25 mg/kg bw)
To convert the dose values the density (1.3375 g/ml) of the substance has been used: y ml/kg x 1.3375 g/ml = z g/kg bw. - No. of animals per sex per dose:
- Range finding assay: 2 animals per group
Final assay: 5 males and 5 females per group. - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days (7 days for the range finding assay)
- Frequency of observations and weighing: Animals were observed immediately following dosing and thereafter at daily interval. Body weight was monitored throughout the study.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight. - Statistics:
- The acute oral LD50 value, together with its 95% confidence limits, was calculated according to the method of Litchfield and Wilcoxon - A Simplified Method of Evaluating Dose - Effect Experiments - J. Pharm. Exp. Tharap. 1949, 96 99.
Results and discussion
- Preliminary study:
- A range-finding study was conducted involving the administration of the product at dose levels equivalent to 0.8, 0.4, 0.2 and 0.1 ml/kg (corresponding to 1070, 535, 267.5 and 133.75 mg/kg) to groups of 2 animals to determine the order of acute oral toxicity with respect to lethal effect. These animals were observed immediately after dosing and then throughout the following 7 day period. Mortality results are presented in table 1 in the field "any other information on results incl. tables".
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 320 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 240.75 - 414.63
- Mortality:
- Yes, mortality occurred during the time course of the test. For full details see table 2 in the field "any other information on results incl. tables".
- Clinical signs:
- other: Within 24 hours of dosing, animals in the higher dose groups (535 and 374.5 mg/kg bw) exhibited severe piloerection, hypotermia, coma, blood on masks and lethargy. However, those rats dosed at levels of 187.25 and 267.5 mg/kg bw displayed only slight pilo
- Gross pathology:
- No gross abnormalities were observed in any of the animals selected.
Any other information on results incl. tables
Table 1: Mortality results of the range finding assay
Dose level (mg/kg bw) | Deaths by day | Percentage Mortality (%) |
||||
1 | 2 | 3 | 4 | 7 | ||
1070 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 100 |
535 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 100 |
267.5 | 0/2 | 0/2 | 1/2 | 1/2 | 1/2 | 50 |
133.75 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0 |
Table 2: Mortality results of the final assay
Dose level (mg/kg bw) | Deaths by day | Percentage Mortality (%) |
||||||
1 | 2 | 3 | 4 | 5 | 7 | 14 | ||
535 | 10/10 | 10/10 | 10/10 | 10/10 | 10/10 | 10/10 | 10/10 | 100 |
374.5 | 5/10 | 6/10 | 6/10 | 6/10 | 6/10 | 6/10 | 6/10 | 60 |
267.5 | 2/10 | 4/10 | 4/10 | 4/10 | 4/10 | 4/10 | 4/10 | 40 |
187.25 | 0/10 | 0/10 | 0/10 | 0/10 | 0/10 | 0/10 | 0/10 | 0 |
Table 3: Group mean body weight data
Dose level (mg/kg bw) | Body weight (g) at day | |||
0 | 3 | 7 | 14 | |
535 | 158.4 | - | - | - |
374.5 | 145.4 | 157.5 | 163.3 | 193.3 |
267.5 | 163.5 | 156.0 | 188.7 | 195.7 |
187.25 | 145.2 | 181.7 | 188.9 | 220.6 |
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- Under the condtions of this test, the results indicated that the test substance should be classified as Acute oral Tox. 4, H302 (Harmful if swallowed) according to CLP .
- Executive summary:
In an acute toxicity test, Wistar rats of both sexes (5 males and 5 females per group) were exposed to 2 -Fluoronitrobenzene. The sample was administered at dose levels equivalent to 535, 374.5, 267.5 and 187.25 mg/kg bw, in order to obtain the 0 - 100 % sequence of lethal effects necessary for the estimation of the acute oral LD50 value. Animals were observed immediately following dosing and thereafter at daily interval for a period of 14 days. Any deaths or other signs of toxicity were recorded.
Body weight was monitored throughout the study, and postmortem examinations were performed on selected individuals to observe any gross abnormalities in the viscera. Severe piloerection, hypotermia, coma, blood on mask, lethargy within 24 hours were observed in 535 and 374.5 mg/kg groups. Slight piloerection after 24 hours, and weight loss in certain individuals after 72 hours were observed in the 267.5 mg/kg group, otherwise all surviving rats were apparently asymptomatic by day 3 and until the end of the experiment.
Mortality occurred in all animals at the highest dose (535 mg/kg bw).
At 374.5 mg/kg bw, 60 % of mortality was observed and at the dose level of 267.5 mg/kg bw 40 % of mortality was observed. At the lowest dose (187.5 mg/kg bw), no mortality was observed.
Then, a LD50 value of 320 mg/kg bw (240.75 - 414.63 mg/kg bw) has been calculated using the method of Litchfield and Wilcoxon. Based on results of this acute test, the substance is classified as Acute oral Tox. 4, H302 (Harmful if swallowed) according to CLP.
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