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EC number: 286-304-6 | CAS number: 85204-10-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to international guideline(s), GLP-compliant, performed in recognized contract research organization, no restrictions, fully adequate for assessment.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: nominal 100 , 50, 25, 12.5, 6.25 mg/L and control
- Sampling intervals: start and end of the first and the last renewal period
- Sampling method: samples were taken from flasks containing no animals
- Sample storage conditions before analysis: no storage prior to analysis - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The highest test concentration of 100 mg/L and dilutions thereof were prepared in test medium. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: “Black Molly” Fish Farm, Veszprém, Hungary
- Length at study initiation: 2.6 – 3.1 cm
- Weight at study initiation (mean and range, SD): 0.36 g ± 0.01 g (0.35-0.38 g)
- Feeding during test: none
ACCLIMATION
- Acclimation period: > 12 days
- Acclimation conditions (same as test or not): same water quality (circulated and filtered tap water)
- Type and amount of food: not reported
- Feeding frequency: daily, until 24 hours before the start of the test
- Health during acclimation (any mortality observed): The fish were healthy. There was no mortality of the population for seven days before the test - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 203 mg/L as CaCO3 (measured)
- Test temperature:
- 23.1-23.6 °C
- pH:
- 7.9-8.1
- Dissolved oxygen:
- 95-96% air saturation value
- Nominal and measured concentrations:
- nominal concentrations: 100, 50, 25, 12.5, and 6.25 mg/L
freshly prepared test media:87-126% of nominal
aged test media (24 h): 100-137% of nominal (94-128% of initially measured)
The analytically measured concentrations deviated more than 20% from the nominal values in some cases, thus the biological results are based on the geometric mean measured concentrations: 108.9, 56.9, 30.1, 15.0, and 6.4 mg/L (102-120% of nominal) - Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria, containing 5 litres of medium
- Type: not reported (the test material is not volatile)
- Aeration: not reported (oxygen concentration was sufficient during exposure)
- Renewal rate of test solution: daily
- No. of organisms per vessel: 7 (except the 50 mg/L test group which contained 8 fish)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.49 - 0.56 g body weight/L (mean 0.52 ± 0.02 g bw/L)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: circulated and filtered tap water
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and sub-lethal effects; recorded at approx. 3, 6, 24, 48, 72 and 96 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0
- Range finding study
- Test concentrations: nominal 100, 10, 1, and 0.1 mg/L
- Results used to determine the conditions for the definitive study: 100% mortality after 96 hours at 100 mg/L; no mortality at the lower test concentrations - Reference substance (positive control):
- not required
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 36 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence limits: 29.3-60.3 mg/L
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: one dead fish after 48 h
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 57 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: swimming problems and decreased activity at the highest test concentration (at the 3-hour observation); no abnormalities observed at any other concentration and/or time
- Mortality of control: zero
- Other adverse effects control: none
For cumulative mortality data Table 1. - Reported statistics and error estimates:
- The 24, 48, 72 and 96 hours LC50 values were calculated by Probit analysis with 95% confidence limits using SPSS PC+ software.
The NOEC, LOEC and LC100 were determined directly from the raw data. - Sublethal observations / clinical signs:
Table 1: Cumulative mortality data:
Concentration (mg/L)*
Cumulative mortality [number dead of fish] **
3 h
6 h
24 h
48 h
72 h
96 h
Control
0
0
0
0
0
0
6.4 (6.25)
0
0
0
0
0
0
15.0 (12.5)
0
0
0
0
0
0
30.1 (25)
0
0
0
1
1
1
56.9 (50)
0
0
0
8
-
-
108.9 (100)
0***
0
3
7
-
-
*: geometric mean measured (nominal)
**: initial population = 7 fish, except 8 fish at nominal 50 mg/L
***: swimming problems and decreased activity
- Validity criteria fulfilled:
- yes
- Remarks:
- no mortality of control animals; concentration of dissolved oxygen in all test vessels >60% saturation
- Conclusions:
- The test material is toxic to freshwater fish with a 96-hour LC50 between 10 and 100 mg/L (calculated value: 36 mg/L).
Reference
Description of key information
96-h LC50 (based on geometric mean concentrations) 36 mg/L for Brachydanio rerio (new name Danio rerio) (OECD 203, EU C.1)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 36 mg/L
Additional information
The results indicate that the test material is toxic to freshwater fish with a 96-hour LC50 between 10 - 100 mg/L (calculated value: 36 mg/L).
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