Registration Dossier
Registration Dossier
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EC number: 303-662-1 | CAS number: 94201-73-7
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
EC50 (aquatic algae; growth rate)= 12mg/L (C.I. 10-14 mg/L), 72 hour, freshwater, OECD 201, Harlan Laboratories Ltd. 2013
EC10 (aquatic algae; growth rate)= 5.8 mg/L, 72 hour, freshwater, OECD 201, Harlan Laboratories Ltd. 2013
NOEC (aquatic algae; growth rate)= 4.4 mg/L, 72 hour, freshwater, OECD 201, Harlan Laboratories Ltd. 2013
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 12 mg/L
- EC10 or NOEC for freshwater algae:
- 4.4 mg/L
Additional information
The algal growth inhibition to Pseudokirchneriella subcapitata, was carried out according to OECD 201 Freshwater Alga and Cyanobacteria, Growth Inhibition Test and EU Method C.3 guidelines. Following a preliminary range-finding test, P.subcapitata was exposed to solutions of the test item at nominal concentrations of 1.0, 3.2, 10, 32 and 100 % (v/v) saturated solution (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1 °C. The test item solutions were prepared by stirring an excess (500 mg/L) of test item in culture medium using a propeller stirrer. After the stirring period any undissolved test item was removed by filtration to produce a 100 % (v/v) saturated solution of the test item. This saturated solution was then further diluted as necessary, to provide the remaining test groups. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group. Analysis of the test preparations at 0 hours showed measured test concentrations to range from 1.9 to 170 mg/L. A decline in measured test concentration was observed at 72 hours and hence it was considered appropriate to calculate the results based on the geometric mean measured test concentration. The 72h-NOEC for growth rate reduction based on GMM concentrations was 4.4 mg/l. The EC50 for growth rate reduction (72h-ERC50) based on GMM concentrations was 12 mg/l (95% CL: 10 - 14 mg/l).
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