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EC number: 253-455-4 | CAS number: 37310-83-1
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The test substance Reaction product of oleylalcohol with polyphosphoric acid was examined in a ready biodegradability study in a closed bottle test (OECD 301D). The test item can be considered to be not ready biodegradable. According to the test guidelines the pass level for ready
biodegradability is removal of 60% of COD.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable, not fulfilling specific criteria
Additional information
The purpose of this study was to determine the ready biodegradability of the test item Reaction product of oleylalcohol with polyphosphoric acid. The test item was exposed to activated sludge from the aeration tank of a domestic waste water treatment plant for 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
The test item Reaction product of oleylalcohol with polyphosphoric acid was investigated in the concentration of 3 mg/L. The concentration was chosen based on the preliminary test results and based on its measured chemical oxygen demand (COD) of 1.97 mg O2/ mg test item. In parallel, positive reference control, Sodium benzoate at the concentration of 3.6 mg/L, inoculum control and procedure control (toxicity control) were investigated. All validity criteria of the study were met.
Under the test conditions the percentage biodegradation of Reaction product of oleylalcohol with polyphosphoric acid reached a mean of 26.1 % after 28 days based on its COD. (The highest percentage biodegradation value, 26.9 % was observed on the 21st day based on COD.) Therefore the test item can be considered to be not ready biodegradable.
The reference item Sodium benzoate was sufficiently degraded to a mean of 61.9 % after 14 days, and to a mean of 70.1 % after 28 days of incubation, based on ThODNH4, thus confirming the suitability of the used activated sludge inoculum.
In the toxicity control containing both, the test item and the reference item, a mean of 39.7 % biodegradation was noted within 14 days and 45.0 % biodegradation after 28 days of incubation. Thus, the test item can be assumed to not inhibit the activated sludge microorganisms (higher than 25% degradation occurred within 14 days).
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