Cyclohexanethiol

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1970

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to a test protocol that is comparable to the appropriate OECD test guideline. It was not compliant with GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexanethiol

EC Number:

216-378-7

EC Name:

Cyclohexanethiol

Cas Number:

1569-69-3

Molecular formula:

C6H12S

IUPAC Name:

cyclohexanethiol

Test material form:

other: liquid

Details on test material:

- Name of test material: Cyclohexyl Mercaptan
No other information reported

Test animals

Species:

rat

Strain:

other: Albino

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 200 to 300 g
- Housing: Standard laboratory rat stock cages
- Diet: ad libitum
- Water: ad libitum

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: All glass exposure chamber
- Exposure chamber volume: 9.5 gallons
- Rate of air: 6.0 l/min
- System of generating vapour: dry air bubbled through 2 flasks containing undiluted cyclohexyl mercaptan connected in series.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Concentrations:

5.60 and 13.3 mg/l

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes

Statistics:

Not performed.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 5.6 - < 13.3 mg/L air

Based on:

test mat.

Exp. duration:

4

Mortality:

Eight out of 10 rats at 13.3 mg/l died during exposure. The remaining 2 died between 2 and 25 hours after the exposure period.
All rats survived at 5.6 mg/l.

Clinical signs:

other: At 13.3 mg/l lethergy and/or general weakness, lacrimation and ataxia were noted within 60 minutes of the start of the exposure period. Rhinorrhea was noted 60 to 90 minutes from start of exposure. There were no remarkable clinical signs in animals at 5.

Body weight:

No information.

Gross pathology:

Hyperemia of liver and lungs and presence of blood in the gastro-intestinal tract were noted for animals at 13.3 mg/l.
There were no remarkable findings in animals at 5.6 mg/l.

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

In an acute vapour inhalation study rats, conducted according to a protocol that is similar to OECD 403, the LC50 was determined to be between 5.6 and 13.3 mg/l. No statistical analysis of the mortality data was performed.