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EC number: 412-280-5 | CAS number: 2511-00-4 POIRENATE
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 13 November to 11 December 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to the OECD Method 301D and it is GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Ethyl 2-cyclohexylpropionate
- EC Number:
- 412-280-5
- EC Name:
- Ethyl 2-cyclohexylpropionate
- Cas Number:
- 2511-00-4
- Molecular formula:
- C11H20O2
- IUPAC Name:
- ethyl 2-cyclohexylpropanoate
- Details on test material:
- - Physical state: clear liquid
- Storage condition of test material: in darkness at 4ºC
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Activated sewage sludge bacteria
- Details on inoculum:
- Source: The aeration stage of the HRC limited sewage treatment plant treating predominantly domestic sewage.
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Details on study design:
- Vessels: 280 ml BOD bottles (darkened glass) with ground glass stoppers.
Test concentrations:
(a) Non-inoculated dilution water.
(b): Inoculated dilution water
(c): Inoculated dilution water plus filter paper.
(d): 2 mg/l test substance plus filter paper.
(e): 3 mg/l sodium benzoate
(f): 2 mg/l aniline plus filter paper.
(g): 2 mg/l test substance plus 3 mg/l sodium benzoate plus filter paper
Test media b,c,d,e,f and g were inoculated with activated sludge filtrate at the rate of 1 drop of inoculum per litre.
Procedure: BOD bottles were filled, by siphnon, and firmly stoppered to exclude all air bubbles. Sufficient bottles were prepared to all a single oxygen determination per bottle to be made at 0,5,15 and 28 days for each test media (a)-(f), (duplicate bottle at each sampling time). Two bottles of preparation (g) were prepared to allow a single oxygen determination per bottle to be made at 28 days.
Bottles were incubated in a water bath at 20±1ºC for 0,5,15 or 28 days as appropriate.
Observation: Dissolved oxygen concentrations for each test medium were determined, in duplicate, at 0,5,15 and 28 days by means of Yellow Springs BOD Probe (Model 50B).
Series (g) containing both the test substance and sodium benzoate was included as a check for possible inhibition effects.
Reference substanceopen allclose all
- Reference substance:
- benzoic acid, sodium salt
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 13
- Sampling time:
- 28 d
- Details on results:
- Dissolved oxygen measurements for the test substance and standard substance solutions, together with the inoculated and non-inoculated blank controls, at 0,5,15 and 28days ere given in Table 1. Mean oxygen depletion values and percentage degradation values (% of ThOD NO3) are given in Table 2.
After 28 days the oxygen depletion value for the inhibition check culture (g) was 102% of the sum of the individual component substance i.e. the test substance plus filter paper plus sodium benzoate (see Table 2). This indicates that no significant inhibition occurred at the test concentration of 2 mg/L.
Oxygen depletions in the inoculated and non-inoculated control series were within the prescribed limits.*
*Non-inoculated control: <0.3 mgO2/L after 5 days, <0.4 mgO2/L after 28 days
Inoculated control: <0.5 mgO2/L after 5 days, <0.6mgO2/L after 28 days.
BOD5 / COD results
- Results with reference substance:
- Sodium benzoate attained 97% biodegradation within 28 days.
Aniline attained 69% biodegradation within 28 days.
Any other information on results incl. tables
TABLE 1 | |||||
Dissolved oxygen measurements (mgO2/L) | |||||
Culture medium | Day | ||||
0 | 5 | 15 | 28 | ||
(a)Dilution water without inoculum | R1 | 8,9 | 8,81 | 8,85 | 8,78 |
R2 | 8,9 | 8,76 | 8,75 | 8,6 | |
Mean | 8,9 | 8,785 | 8,8 | 8,69 | |
(b) Dilution water with inoculum | R1 | 8,9 | 8,7 | 8,82 | 8,45 |
R2 | 8,9 | 8,86 | 8,77 | 8,51 | |
Mean | 8,9 | 8,78 | 8,795 | 8,48 | |
( c) Dilution water with inoculum | R1 | 8,9 | 8,69 | 8,64 | 8,6 |
R2 | 8,9 | 8,65 | 8,64 | 8,59 | |
Mean | 8,9 | 8,67 | 8,64 | 8,595 | |
(d) Test substance (2mg/l) plus filter | R1 | 8,9 | 8,73 | 8,69 | 7,91 |
R2 | 8,9 | 8,75 | 8,78 | 7,92 | |
Mean | 8,9 | 8,74 | 8,735 | 7,915 | |
( e) Standard substance (3mg/l) Sodium benzoate | R1 | 8,9 | 5,31 | 5,12 | 3,47 |
R2 | 8,9 | 5,41 | 4,97 | 3,79 | |
Mean | 8,9 | 5,36 | 5,045 | 3,63 | |
(f) Standard substance (2 mg/l) Aniline plus filter paper | R1 | 8,9 | 8,51 | 5,38 | 4,27 |
R2 | 8,9 | 8,46 | 5,21 | 4,39 | |
Mean | 8,9 | 8,485 | 5,295 | 4,33 | |
(g) Test substance (2mg/l) plus filter paper plus standard substance sodium benzoate (3 mg/l) | R1 | 8,9 | N/A | N/A | 3,11 |
R2 | 8,9 | N/A | N/A | 2,83 | |
Mean | 8,9 | N/A | N/A | 2,97 | |
R1 and R2 Replicates 1 and 2 | |||||
N/A Not applicable | |||||
TABLE 2 | |||||
Mean oxygen depletion and percetage biodegradation values | |||||
Culture medium | Day | ||||
5 | 15 | 28 | |||
(a) Dilution water without inoculum | } O2depletion (mgO2/l) | 0,115 | 0,1 | 0,21 | |
(b) Dilution water with inoculum | } O2depletion (mgO2/l) | 0,12 | 0,105 | 0,42 | |
( c) Dilution water with inoculum and filter paper | } O2depletion (mgO2/l) } |
0,23 | 0,26 | 0,305 | |
(d) Test substance (2 mg/l) plus filter paper | } O2depletion (mgO2/l) } |
<0,070> <1> |
<0,095> <2> |
0,680 13 |
|
( e) Standard substance (3mg/l) Sodium benzoate | } O2depletion (mgO2/l) } % degradation |
3,420 68 |
3,750 75 |
4,850 97 |
|
(f) Standard substance (2 mg/l) Aniline plus filter paper | } O2depletion (mgO2/l) } % degradation |
0,185 3 |
3,345 54 |
4,265 69 |
|
(g) Test substance (2 mg/l) plus filter paper plus standard sodium benzoate (3 mg/l) | } O2depletion (mgO2/l) } } |
N/A | N/A | 5,625 |
<Increase>
N/A Not applicable
(i) Test substance
Oxygen depletion= (a-d)-(a-c) from Table 1
(ii) Standard substance (sodium benzoate)
Oxygen depletion = (a-e)-(a-b) from Table 1
(iii) Standard substance (aniline)
Oxygen depletion = (a-f)-(a-c) from Table 1
(iv) Inoculated and non-inoculated dilution water (plus filter paper blank)
Oxygen depletion=initial O2 concentration - measured value
(v) % Degradation= Oxygen (depletion/conc.of substance x ThOD NO3 or COD) x 100
(vi) Inhibition check
Oxygen depletion = (a-g)-(a-c) from Table 1
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- The test substance cannot be termed as readily biodegradable.
- Executive summary:
The substance was tested for ready biodegradability accroding to the OECD 301D Closed-Bottle-Test. The test substance concentration was 2mg/, the oxygen depletion after 28 days was 0.68 mg O2/L and the theoretical oxygen demand was 5.22 mgO2/L. Under the chosen conditions a degradation of 13% was measured after 28 days. The substance cannot be termed as readily biodegradable.
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