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EC number: 263-326-4 | CAS number: 61931-53-1 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 12195 and 12197.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- other: experimental data on similar substance
- Adequacy of study:
- key study
- Study period:
- 19 Oct 2015 to 20 Oct 2015
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 2008
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct addition by initial weight into the test flasks. The test substance was weighed in the required amounts for the test concentrations directly to the test vessels. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Origin of the test system: aeration tank of the wastewater treatment plant of Mannheim, Germany
- Collection of the test system: 19 Oct 2015
- Arrival in the test facility: 19 Oct 2015
- Preparation of inoculum for exposure: After arrival of the activated sludge suspension in the test facility the suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed once with drinking water and the suspension was adjusted to 3 g/L Dw. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21.4 - 21.5 °C
- pH:
- Start: 7.4 - 7.5
After incubation time: 8.0 - 8.1 - Dissolved oxygen:
- 5.6 - 8.8 mg/L
- Nominal and measured concentrations:
- - Nominal concentrations: 0 (control), 62.5, 125, 250, 500 and 1000 mg/L
- Details on test conditions:
- TEST PREPARATION
The test substance was added in the required amounts according to the test concentrations directly to the test vessels. Aliquots of the reference substance stock solution were dosed to the test vessels and made up with deionized water . Synthetic medium were dosed to each test vessel with test substance and reference substance afterwards. To prepare the blank control assays, deionized water and synthetic medium were mixed. The pH-values in all test vessels were checked. An adjustment was not necessary.
TEST SYSTEM
- Test vessel: glass-beakers (nominal volume 1L)
- Test volume: 500 mL
- Synthetic medium: 16 mL/test vessel of 100-fold concentrated OECD medium
- Replicates: 6 for the control, 3 for each test substance concentration, 2 for each reference substance concentration
- Duration of measurement during oxygen consumption: about 8 - 10 minutes
- Sludge concentration in the test vessel: 1.5 g/L Dw
OTHER TEST CONDITIONS
- Adjustment of pH: adjustment of pH was not necessary
EFFECT PARAMETERS MEASURED:
- The oxygen consumption was measured over a period of 8 - 10 minutes. The 5.5 - 6.7 minutes from the plotted track within a linear range of the graph was used for the determination of the respiration rate (mg O2/L*h) and compared to a blank control.
- Other: The pH-values in all test vessels were measured at test start and after incubation.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: 95% C.L.: 252.6 - 407.0 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- A dose-related inhibition of respiration by the test substance was observed, see ‘Any other information on results incl. tables’.
- Results with reference substance (positive control):
- The 3-h EC50 of the reference substance 3,5-dichlorophenol was determined to be 9.0 mg/L.
- Reported statistics and error estimates:
- The consumption rates were used for the determination of the ECx by the probit method based on Finney (Finney, D.J., Probit Analysis; Cambr. Univ. Press, 3rd ed., 1971) with the software TOXRAT Professional 2.10 (Ratte, M.: ToxRat Professional 2.10 (2001-2010). ToxRat Solutions GmbH, Naheweg 15, 52477 Alsdorf, Germany).
- Validity criteria fulfilled:
- yes
- Conclusions:
- Not toxic to microorganism
IC50 > 100 mg/l
Reference
Table: Measured data of oxygen content of the test assays at the begin and end of the evaluation time
Assay identification |
BC1 |
BC2 |
BC3 |
BC4 |
BC5 |
BC6 |
RS1 |
RS2 |
RS3 |
RS4 |
RS5 |
RS6 |
Reference substance concentration; [mg/L] |
- |
- |
- |
- |
- |
- |
1 |
10 |
100 |
|||
O2 conc. Start [mg/L] |
5.6 |
6.3 |
7.4 |
7.4 |
7.5 |
8.0 |
8.0 |
7.0 |
8.3 |
8.5 |
8.8 |
8.7 |
O2 conc. End [mg/L] |
1.7 |
2.5 |
3.6 |
3.4 |
3.5 |
4.3 |
4.5 |
3.5 |
6.6 |
6.6 |
8.4 |
8.2 |
O2 consumption rate [mg/L] |
3.9 |
3.8 |
3.8 |
4.0 |
4.0 |
3.7 |
3.5 |
3.5 |
1.7 |
1.9 |
0.4 |
0.5 |
O2 consumption in minutes |
6.1 |
6.1 |
6.0 |
6.1 |
6.2 |
5.9 |
6.2 |
6.4 |
6.0 |
6.0 |
5.5 |
6.5 |
O2 consumption rate [mg/L×h] |
38 |
37 |
38 |
39 |
39 |
38 |
34 |
33 |
17 |
19 |
4 |
5 |
Specific O2 consumption rate [mg/g×h] |
25 |
25 |
25 |
26 |
26 |
25 |
23 |
22 |
11 |
13 |
3 |
3 |
Calculation of inhibition respiration [%] |
- |
11 |
14 |
55 |
50 |
90 |
87 |
Assay identification |
TS1 |
TS2 |
TS3 |
TS4 |
TS5 |
TS6 |
TS7 |
TS8 |
TS9 |
TS10 |
TS11 |
TS12 |
TS13 |
TS14 |
TS15 |
Test substance concentration; [mg/L] |
62.5 |
125 |
250 |
500 |
1000 |
||||||||||
O2 conc. Start [mg/L] |
6.5 |
7.4 |
7.0 |
6.2 |
6.6 |
7.5 |
7.1 |
6.3 |
7.0 |
7.7 |
7.5 |
6.7 |
6.7 |
7.7 |
7.5 |
O2 conc. after x minutes [mg/L] |
2.5 |
3.5 |
3.0 |
2.5 |
2.8 |
3.7 |
3.3 |
2.5 |
3.3 |
4.7 |
4.3 |
3.5 |
4.1 |
5.1 |
4.8 |
O2 consumption in minutes |
6.5 |
6.0 |
6.1 |
6.0 |
6.1 |
6.0 |
6.0 |
6.2 |
6.3 |
6.0 |
6.0 |
6.7 |
6.5 |
6.0 |
6.0 |
O2 consumption rate [mg/L] |
4.0 |
3.9 |
4.0 |
3.7 |
3.8 |
3.8 |
3.8 |
3.8 |
3.7 |
3.0 |
3.2 |
3.2 |
2.6 |
2.6 |
2.7 |
O2 consumption rate [mg/L×h] |
37 |
39 |
39 |
37 |
37 |
38 |
38 |
37 |
35 |
30 |
32 |
29 |
24 |
26 |
27 |
Specific O2 consumption rate [mg/g×h] |
25 |
26 |
26 |
25 |
25 |
25 |
25 |
25 |
23 |
20 |
21 |
19 |
16 |
17 |
18 |
Calculation of inhibition respiration [%] |
3 |
-2 |
-2 |
3 |
3 |
0 |
0 |
3 |
8 |
21 |
16 |
24 |
37 |
32 |
29 |
BC = blank control, RS = reference substance, TS = test substance
Table: Obtained effect concentrations
Toxicity Response Variable |
Based on nominal concentration [mg/L] |
Confidence Limits (95%) lower/upper |
EC10 |
320 |
252.6/407.0 |
EC20 |
570 |
498.0/656.1 |
EC50 |
> 1000 |
- |
EC80 |
> 1000 |
- |
Description of key information
The 3-h EC50 and EC10 values are > 1000 mg/L and 320 mg/L, respectively, as observed in a respiration inhibition study with activated sludge of a predominantly domestic sewage.
Key value for chemical safety assessment
Additional information
The toxicity of the substance to microorganisms was investigated in an activated sludge respiration inhibition study according to OECD TG 209 and in compliance with GLP criteria (BASF, 2016). In this study, non-adapted activated sludge from a predominantly domestic sewage was exposed to nominal test substance concentrations of 0 (control), 62.5, 125, 250, 500 and 100 mg/L for 3 hours. Test concentrations were not analytically verified. The effects on microorganisms was determined by measuring total respiration rates and comparing these to an unexposed control. Inhibition of respiration rates increased concentration-dependently reaching 33% at the highest concentration tested. Based on these findings, the 3-h EC50 and EC10 values were determined at > 1000 mg/L and 320 mg/L, respectively.
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