Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 233-036-2 | CAS number: 10025-67-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with restrictions (Only 4 strains were tested. The hydrolised product of disulphur dichloride were tested).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- Only 4 strains were tested. The hydrolised product of disulphur dichloride were tested
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Disulphur dichloride
- EC Number:
- 233-036-2
- EC Name:
- Disulphur dichloride
- Cas Number:
- 10025-67-9
- Molecular formula:
- Cl2S2
- IUPAC Name:
- dichlorodisulfane
- Details on test material:
- - Name of test material (as cited in study report): sulphur chloride (hydrolysed)
- Physical state: liquid
- Analytical purity: 99.9%
- Lot/batch No.: 5442, production dated 11 June 1987
- Storage condition of test material: the test substance was stored in refrigerator.
- Other: disulphur dichloride was tested in the form of its hydrolysis products in aqueous solution. 1 L solution of hydrolysis products contains 97 g disulphur dichloride.
The batch of disulphur dichloride used to produce the hydrolysis products and the solution of hydrolysis products were analytically examined and released for at least the test period. The hydrolysis products were stored under prevention from air using N2. Before use or dilution the aqueous solution of hydrolysis products was centrifuged and filtered to separate from precipitates. Afterwards the solution was filter-sterilized and prevented from air by N2.
Deionized water for dilutions was blown free of air by treatment with N2 for at least 30 minutes. The dilution of the aqueous solution of hydrolysis products using the deionized water was stable.
Constituent 1
Method
- Target gene:
- his operone
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: strains are partly deficient in lipopolysaccharide side chain
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- first test: 0, 1.3, 6.2, 31.2, 156.0, 780 µg per plate
1st repeated test: 0, 12.8, 64.0, 320.0, 1600, 8000 µg per plate
2nd repeated test: 0, 500, 1000, 2000, 4000, 8000 µg per plate - Vehicle / solvent:
- the solvent used for sulfur chloride (hydrolysed) was deionized water, and for the positive controls DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- deionized water
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: sodium azide (only TA 1535; -S9), nitrofurantoin (only TA 100; -S9), 4-nitro-1,2-phenylenediamine (TA 1537 + TA 98; -S9), 2-aminoanthracene (all strains; +S9 mix).
- Details on test system and experimental conditions:
- Ames test
METHOD OF APPLICATION: in agar (plate incorporation)
DETERMINATION OF CYTOTOXICITY
- Method: The toxicity of the substance was assessed in 3 ways. First, the background growth on the plates for the mutant determination was grossly appraised. Secondly, a toxic effect of the substance was assumed when the mutant count per plate was clearly lower than the negative control count in dose correlation. Thirdly, the titer was determined. If this is indicated in the tables under the heading "Titer", the total bacteria count was taken on two plates per concentration with S9 mix. However, if evaluation was performed only without S-9 mix, the bacterial count was taken on two plates per concentration without S9 mix.
- Evaluation criteria:
- A reproducible and dose-related increase (ie. > twice the negative control count) in mutant counts for at least one strain is considered positive. Otherwise, the result is evaluated as negative
- Statistics:
- no data
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Up to 156 µg, 64 µg, 500 µg (respectively in the 1st, 2nd, and 3rd experiment) per plate no bacteriotoxic effect was observed. At high doses, the substance had a weak-strain-specific bacteriotoxic effect (not used for evaluation purposes)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- no further data
- Remarks on result:
- other: strain/cell type: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Maximum number of revertants(means ± SD):
Trial 1 (1.3 - 780.0 µg/plate) |
||||
|
Control |
Test Substance (µg/plate) |
||
Strain |
-S9 |
+S9 |
-S9 |
+S9 |
TA1535 |
12 |
11 |
11 (6.2; 780.0) |
16 (780.0) |
TA100 |
87 |
131 |
99 (6.2) |
147 (156.0) |
TA1537 |
20 |
19 |
26 (31.2) |
21 (780.0) |
TA98 |
17 |
37 |
24 (780.0) |
43 (156.0) |
Trial 2 (12.8 - 8000.0 µg/plate) |
||||
TA1535 |
12 |
15 |
14 (64.0; 320.0; 8000.0) |
20 (320.0) |
TA100 |
98 |
139 |
108 (320.0) |
148 (64.0) |
TA1537 |
11 |
20 |
16 (1600.0) |
19 (1600.0) |
TA98 |
93 |
90 |
125 (8000.0) |
116 (1600.0) |
Trial 3 (500 - 8000.0 µg/plate) |
||||
TA1535 |
16 |
15 |
18 (500.0) |
18 (1000; 4000) |
TA100 |
97 |
160 |
115 (2000) |
151 (4000) |
TA1537 |
14 |
12 |
13 (500) |
14 (500; 2000; 4000) |
TA98 |
18 |
25 |
19 (500.0; 1000.0; 2000.0; 8000.0) |
37 (4000) |
Conclusion:
The test substance was not genotoxic in bacteria up to a concentration of 8000 µg/plate with and without metabolic activation.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation - Executive summary:
Herbold BA (1988)
The mutagenic potential of disulphur dichloride (hydrolysed) was investigated in a Salmonella/microsome test in doses up to 8000 µg per plate on four Salmonella typhimurium LT2 mutants according to the OECD guideline 471 with restrictions ( Only 4 strains were tested. The hydrolised product of disulphur dichloride were tested).
For the test were used the histidine-auxotrophic strains TA 1535, TA 100, TA 1537 and TA 98.
Doses up to and including 156 µg per plate did not cause any batteriotoxic effects. The total bacteria counts remained unchanged. No inhibition of growth was observed.
At higher doses, the substance had a weak strain-specific bacteriotoxic effect, so that this range could nevertheless be used for evaluation purposes.
Evidence of mutagenic activity of disulphur dichloride (hydrolysed) was not found with and without metabolic activation.
Neither a dose- related doubling, nor a biologically relevant increase in the mutant count, in comparison with the negative controls, was observed.
The positive controls sodium azide (Na-azid) , nitrofurantoin (NF), 4-nitro-1,2-phenylene-diamine (4 -NPDA), and 2-aminoanthracene (2 -AA), had a marked mutagenic effect, as was seen by a biologically relevant increase in mutant colonies compared to the corresponding negative controls.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.