2-Butene, 1,1,4,4-tetramethoxy-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

8.- 12.10.1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Similar to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1991

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not applicable

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(2E)-1,1,4,4-tetramethoxybut-2-ene; (2Z)-1,1,4,4-tetramethoxybut-2-ene

EC Number:

900-110-7

Cas Number:

5370-08-1

Molecular formula:

C8H16O4

IUPAC Name:

(2E)-1,1,4,4-tetramethoxybut-2-ene; (2Z)-1,1,4,4-tetramethoxybut-2-ene

Details on test material:

- Name of test material (as cited in study report): C4-Diacetale
- Physical state: liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

-

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the 96-hour acute toxicity study the NEC of C4-Diacetal in rainbow trout is 100 mgll, the LC100 is 300 mg/L and the LC50 is 192 mg/l.
Thus, C4-Diacetal is slightly toxic for fish.

Executive summary:

The acute toxicity of C4 -Diacetal for Fish was estimated by continous exposure of rainbow trout for 96 hours, according to the GLP Standard Operational Procedure "FT1990". TSU. Following an acclimation and adaption period. groups of 10 trout oE average length 6.5 cm were exposed under controlled conditions in 30-litre glass tanks using a static test system to the following nominal concentrations of the test compound: 60, 100, 170. 300 and 500 mg/litre. As controls a group of the fish was exposed to normal urban water. The Fish were observed daily. Based on the 96-hour acute toxicity study of C4-Diacetal in rainbow trout the LC50 is 192 mg/L (NEC 100 mg/l, LC100 300 mg/l). Thus, C4-Diacetal is slightly toxic for fish. The test substance has a solubility in water of > 1000 mg/l.