Registration Dossier
Registration Dossier
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EC number: 223-786-9 | CAS number: 4073-98-7
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.
Key value for chemical safety assessment
Additional information
No experimental data are available for 4,4'-methylenebis(2,6-xylidine) (CAS 4073 -98 -7). To assess the toxicity to fish, read across was made to the analogue substances 4,4'-methylenebis(2-methylaniline) (CAS 838 -88 -0) and N,N,N',N'-tetramethyl-4,4'-methylenedianiline (CAS 101 -61 -1).
Two studies were made to assess the toxicity of 4,4'-methylenebis(2-methylaniline) (CAS 838 -88 -0) to microorganisms. The available results do not indicate any inhibitory effects at the tested concentrations, i.e. up to the maximum water solubility of ca. 16 mg/L.
In an O2 -consumption test with Pseudomonas putida (Robra-test) the 30 -min EC10 and EC50 were determined to be 9000 mg/L and > 10000 mg/L, respectively, based on nominal concentrations (BASF AG, 1988, project no.: 10/0248/88). In a short-term respiration study with industrial activated sludge performed according to ISO 8192 (Test for Inhibition of Oxygen Consumption by Activated Sludge) was made. Based on nominal cocnetrations, an EC20 of > 1995 mg/L was observed (BASF AG, 1981, project no.: 38/81).
In addition the toxicity of N,N,N',N'-tetramethyl-4,4'-methylenedianiline to microorganisms was tested according to OECD 209 using activated sludge of a predominantly domestic sewage. No significant inhibition of respiration was measured up to the highest nominal concentration of 1000 mg/L (BASF AG, 1998, 97/0545/08/1).
Following this, the inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.
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