Hydroxy(2-methylprop-2-enoato-O)zinc

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro DNA damage and/or repair study

Remarks:

Type of genotoxicity: DNA damage and/or repair

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: NTP-study, method and results are sufficiently described.

Data source

Reference

Reference Type:

publication

Title:

Chromosome aberration and sister chromatid exchange test results with 42 chemicals

Author:

Anderson BE, Zeiger E, Shelby MD, Resnick MA, Gulati DK, Ivett JL, Loveday KS

Year:

1990

Bibliographic source:

Environ. Mol. Mutagen. 16: 55-137

Materials and methods

Principles of method if other than guideline:

Sister chromatid exchange in CHO cells

GLP compliance:

not specified

Type of assay:

sister chromatid exchange assay in mammalian cells

Test material

Test material information

Constituent 1

Reference substance name:

Methyl methacrylate

EC Number:

201-297-1

EC Name:

Methyl methacrylate

Cas Number:

80-62-6

IUPAC Name:

methyl methacrylate

Method

Species / strain

Species / strain / cell type:

other: CHO cells

Metabolic activation:

with and without

Metabolic activation system:

AROCLOR 1254 induced rat liver S9 mix

Test concentrations with justification for top dose:

1250 ug/ml - 5000 ug/ml

Results and discussion

Test results

Species / strain:

Chinese hamster Ovary (CHO)

Metabolic activation:

with and without

Genotoxicity:

ambiguous

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
ambiguous clastogenic at high toxic doses

As clastogenic activity was found at probably high toxic doses, it is not possible to conlude that methyl methacrylate has a real direct clastogenic effect as it is probably secondary to cytotoxicity.

Executive summary:

In a cytogenetic test with CHO cells induction of chromosomal aberrations was bound to high doses which are assumed to be strongly cytotoxic. With S-9 mix treatment was for 2 h followed by 8 to 10 h recovery. Doses up to 1,600 μg/ml were negative, at 5000 μg/mL the frequency of aberrant cells was 30%; only one experiment was performed. Without S-9 mix, treatment time was 8 hours with 2.0 to 2.5 h recovery. Doses up to 500 μg/mL were negative, at 1600 and 3000 μg/mL aberration frequencies ranging from 5 to 10% were found. Data on cytotoxic effects were not given, however, it can be assumed from the data presentation and the general approach of the authors that the highest doses tested led to strong cytotoxic effects. Thus, methyl methacrylate showed clastogenic activity but at highly toxic doses therefore probably due to cytotoxicity more than a direct clastogenic effect.