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Diss Factsheets
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EC number: 904-304-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to O.E.C.D. Test Guideline No. 202 with GLP compliance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (1E)-1,3-dichloroprop-1-ene; 1,2-dichloropropane
- EC Number:
- 904-304-2
- Molecular formula:
- Multi-constituent: C3H4Cl2 and C3H6Cl2
- IUPAC Name:
- (1E)-1,3-dichloroprop-1-ene; 1,2-dichloropropane
- Reference substance name:
- Crude 1,3-dichloropropene
- IUPAC Name:
- Crude 1,3-dichloropropene
- Details on test material:
- As per IUCLID5 Sections 1.1. - 1.4.
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- not specified
- Details on test solutions:
- A concentrated stock solution of the test substance was be prepared as appropriate in defined Elendt M4 dilution medium. This stock solution was be diluted to achieve the required nominal test substance concentrations.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The Daphnia magna (Straus) strain held at Huntingdon Life Sciences was supplied by Sheffield University, having been originally obtained from the National Institute for Applied Chemical Research (IRCHA), France. Daphnia cultures were established with juvenile Daphnia taken from existing cultures after the first brood had been removed and discarded. Cultures are maintained in defined Elendt M4 dilution medium (between 500 and 1500 mL) in glass beakers with glass or perspex covers. The cultures were maintained in a temperature controlled laboratory at 20 +/- 2 degrees C. The day-length was controlled giving a photoperiod of 16 hours light, provided by overhead fluorescent tubes, and 8 hours dark. Daphnia cultures were fed daily with suspensions of the unicellular green alga
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- no data
- Test temperature:
- 20 +/- 2 degrees C
- pH:
- No data
- Dissolved oxygen:
- > 3 mg/L
- Salinity:
- Freshwater
- Nominal and measured concentrations:
- Nominal test substance concentrations were: 0, 0.4.3, 0.94, 2.1, 4.55 and 10 mg/L
- Details on test conditions:
- The prepared test substance dilutions in defined medium were be placed into the test vessels. The test vessels were not aerated during the test. The vessels were of sufficient size to meet the minimum guideline criterion of one Daphnia per 2 mL of test substance dilution. Twenty Daphnia, less than 24 hours old, were used at each test substance concentration in the definitive test. Five Daphnia were placed in each vessel. The Daphnia were not fed during the exposure. Observations were made after 24 and 48 hours of exposure and the numbers of floating, mobile and immobile Daphnia were recorded. For the test to be valid, the number of floating and/or immobile Daphnia in the control group must not exceed 10% at the end of the test.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 5.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- immobilization
- Remarks on result:
- other: 4.3-6.1 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 2.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- immobilization
- Remarks on result:
- other: 2.35-3.34 mg/L
- Details on results:
- Significant complete immobilization (20/20) was observed at a nominal Crude 1,3-dichloropropene concentration of 10 mg/L at 24 hr of exposure. Complete immobilization of the Daphnids was observed at 4.55 and 10 mg/L following 48 hr of exposure. Immobilization was 1/20 at 2.1 mg/L and 6/20 at 4.55 mg/L at 24 hr. The immobilization was 1/20 at 0.94 and 2.1 mg/L after 48 hr of exposure.
- Results with reference substance (positive control):
- No data
- Reported statistics and error estimates:
- Apprpriate statistical methods including 1-tailed Fisher's exact test were used to determine significance, estimate EC50 values and to calculate the 95% Confidence Intervals.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Crude 1,3-dichloropropene exposure induced an EC50 value of 2.8 mg/L (2.35-3.34 mg/L 95% Confidence Limits) in Daphnia following 48 hr of exposure based on immobilization. This finding suggests that Crude 1,3-dichloropropene is "toxic" to aquatic organisms.
- Executive summary:
Crude 1,3-dichloropropene was assessed for acute toxicity to the water flea Daphnia magna in an O.E.C.D. Test Guideline No. 202 study with GLP compliance. Complete immobilization of the Daphnids was observed at 4.55 and 10 mg/L after 48 hr of exoposure. Crude 1,3-dichloropropene induced an EC50 value of 2.8 mg/L in Daphnia following 48 hr of exposure based on immobilization. This finding suggests that Crude 1,3-dichloropropene is "toxic" to aquatic organisms.
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