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EC number: 257-885-3 | CAS number: 52372-39-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Water solubility
Administrative data
Link to relevant study record(s)
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- December 20, 2017 - April 06, 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method A.6 (Water Solubility)
- Deviations:
- no
- GLP compliance:
- no
- Type of method:
- column elution method
- Key result
- Water solubility:
- 11 µg/L
- Conc. based on:
- test mat.
- Loading of aqueous phase:
- 1.03 g/L
- Temp.:
- 20 °C
- pH:
- > 5.6 - < 5.9
- Executive summary:
The water solubility cS of the test item Disperse Red 277 at a temperature of 20 °C was determined according to the column elution method. It was found to
cS = 0.011 mg/L
Reference
Preliminary test
In a stepwise procedure, increasing volumes of demineralized water were added at room temperature to a defined amount of the test item. After each addition of an amount of water the mixture was treated with ultrasound for 10 minutes and visually checked for any undissolved particles.
Table4: Results of the preliminary test
Amount of test item / mg |
Added volume of demineralized water / mL |
Total volume of demineralized water / mL |
Appearance of mixture |
102.7 |
0.1 |
0.1 |
Dry red powder, not dissolved |
0.4 |
0.5 |
Cloudy solution, particles, not dissolved |
|
0.5 |
1.0 |
not dissolved |
|
1.0 |
2 |
not dissolved |
|
8.0 |
10 |
not dissolved |
Three additional preliminary tests were carried out with specified amounts of the test item and 100 mL and 500 mL demineralized water, respectively. After each addition of an amount of water, the mixture was stirred for at least 24 hours, and visually checked for any undissolved particles.
Table5: Results of the preliminary test
Amount of test item / mg |
Total volume of demineralized water / mL |
Appearance of mixture |
11.7 |
100 |
Red coloured solution with particles |
10.5 |
500 |
Particles stuck at the agitator and swam on surface |
3.0 |
500 |
Particles stuck at the agitator and swam on surface |
The preliminary test showed that the water solubility of the test item is < 10 mg/L. According to the preliminary test the water solubility was determined by the column elution method in the main test.
Column elution method
103.0 mg of the test item were dissolved in 100 mL tetrahydrofuran using the ultrasonic bath. For charging the columns in each case 25 mL of the test item solution was added to about 3 g of sea sand and the mixtures were shaken. The solvent was then removed under reduced pressure using a rotary evaporator (40 °C, approx. 30 mbar). The dry residues were placed into the columns of the test apparatus, which have afterwards been filled with water (test temperature: 20 °C; water pH at 23 °C: 5.5). After a swelling time of 2 h pumps were started to rinse the test item coated sand with demineralized water. At the end of the study it was confirmed that residual test item remained on the columns. The residual test item was eluted with 50 mL THF. As blank value the used demineralized water was tested. No signal was detected in the range of the retention time of the test item. The concentration of the test item was quantified by HPLC. Refer to Table6 to Table8 for results.
Column 1:
Rotation period of the fraction collector: 90 min per fraction
Duration of sampling: about 71 h (47 fractions)
Table6: Column elution method, column 1 (approximately 12.5 mL/h)
Fraction no. |
V / mL |
pH |
water solubility /µg/L |
40 |
19.0 |
5.6 |
12.99 |
41 |
19.0 |
5.7 |
13.80 |
42 |
19.0 |
5.8 |
9.16 |
43 |
19.5 |
5.9 |
9.00 |
44 |
19.5 |
5.7 |
9.88 |
The water solubility was found to be 11.00 µg/L.
Column 2:
Rotation period of the fraction collector: 45 min per fraction
Duration of sampling: about 66 h (88 fractions)
Table7: Column elution method, column 2 (approximately 25.0 mL/h)
Fraction no. |
V / mL |
pH |
water solubility /µg/L |
83 |
17.3 |
5.8 |
10.14 |
84 |
16.7 |
5.6 |
10.74 |
85 |
16.7 |
5.9 |
12.30 |
86 |
16.0 |
5.9 |
12.22 |
87 |
15.3 |
5.9 |
10.25 |
The water solubility was found to be 11.1 µg/L.
In the second column the concentration of five consecutive fractions was constant within ± 30 %. No increasing or decreasing tendency was observed. The first column showed a variation of the measured concentration of 35%. As for column 1, seven additional analyzed fractions (fraction 22-27) were also in the range of 9.5 mg/L to 14.6 mg/L, the variation of >30% had no influence on the final results of the water solubility experiment. The results of the first and second column were in the same order of magnitude.
Coating check:
Table8: Coating check
|
Amount of test item used for coating / mg |
Measured concentration of test item in eluate / mg/L |
Amount of test item eluted / mg |
Column 12.5 mL/h |
25.8 |
0.360 / 0.361 |
18.0 |
Column 25.0 mL/h |
0.344 / 0.344 |
17.2 |
The coating check was successful. Enough test item remained on the columns.
The water solubility cS of the test item Disperse Red 277 was 11.1 µg/L (mean value of the columns).
Description of key information
The water solubility cS of the test item Disperse Red 277 at a temperature of 20 °C was determined according to the column elution method. It was found to
cS = 0.011 mg/L
Key value for chemical safety assessment
- Water solubility:
- 0.011 mg/L
- at the temperature of:
- 20 °C
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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