1-(N,N-bis(2-ethylhexyl)aminomethyl)-1,2,4-triazole

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May, 1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

(1984)

Deviations:

yes

Remarks:

Fish size: 29 - 32 mm (average; 30 mm)

GLP compliance:

no

Remarks:

but GLP like quality assurance

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

1000 mg of the test item were dissolved in DMF / 0.2 mL HCl 37% / 1 mL NaOH 0.1 N and made up to 10 ml with DMF. By dilution
with water a solution of 50 mg/L was obtained.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: ZEBRA FISH (Brachydanio rerio)
- Source:West Aquarium / D-3422 Bad Lauterberg
- Length at study initiation (length definition, mean, range and SD): 30 mm (29 - 32 mm)
- Weight at study initiation (mean and range, SD): 0.27 g (0.24-0.31 g)

ACCLIMATION
- Acclimation period: 158 days

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

183 mg/L CaCO3

Test temperature:

23 °C

pH:

7.7-8.1

Nominal and measured concentrations:

0.32, 0.58, 1.00, 1.80, 3.20, 5.80 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria of 20 liters filled with 15 liters (36/22/25 cm)
- Aeration: gentle aeration during the test
- No. of organisms per vessel: 10 fishes per concentration and control, 10 fishes per aquariun

OTHER TEST CONDITIONS
- Photoperiod: Fluorescent light , 16 hours daily

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Controls :
Mortalities blank 0%
Mortalities vehicle 0%

Conclusions:

The 96-h LC50 of the substance EC 401-280-0 and its hydrolysate was determined to be 1.1 mg/L under static conditions

Description of key information

The 96-h LC50 of the substance EC 401-280-0 and its hydrolysate was determined to be 1.1 mg/L under static conditions

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

1.1 mg/L

Additional information

Substance EC 401-280-0

The acute toxicity to fish was assessed with substance EC 401-280-0 according to the OECD-guideline no. 203 (1984). The LC50 (96h) is 1.1 mg/L, based on nominal concentrations.

Hydrolysis products:

bis(2-ethylhexyl)amine

In a supporting study conducted with hydrolysis product bis(2-ethylhexyl)amine in 1983, the toxicity of the test substance on the Golden orfe was determined. In this test acetone was used as a solvent. The LC50 was >1.5 and < 2.2 mg/L (nominal value, BASF AG 83/228, 1983). Another supporting study (BASF SE 17F0454/11E135, 2013) was a screening test conducted with Danio rerio based on OECD 203. In this limit test, all fish died at the nominal concentration of 5 mg/L after 24 h of exposure.

 

1H-1,2,4-triazole

No data on acute toxicity on fish with 1H-1,2,4 -triazole are available. However, under environmental conditions the sodium salt of 1H-1,2,4-triazole reacts with water, and 1H-1,2,4-triazole can be regarded as its dissociation product. Read-across data with data on the sodium salt of 1H-1,2,4-triazole show a LC50 96h of 97mg/L (ECHA, 2014). A similar result is expected for 1H-1,2,4-triazole.

 

Formaldehyde

Acute toxicity to fish of formaldehyde was determined in a study with striped bass (Morone saxatilis) and a LC50 of 6.7 mg/L was derived (Wellborn 1969).

 

Therefore, the substance EC 401-280-0 is considered as acutely toxic to fish