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EC number: 940-284-1 | CAS number: 1591782-62-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-10-31 to 2013-11-06
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- D-Glucitol, 1-deoxy-1-(methylamino)-, N-[C8-10(even numbered) acyl] derivs.
- EC Number:
- 940-284-1
- Cas Number:
- 1591782-62-5
- Molecular formula:
- C15H31NO6 C17H35NO6
- IUPAC Name:
- D-Glucitol, 1-deoxy-1-(methylamino)-, N-[C8-10(even numbered) acyl] derivs.
- Reference substance name:
- Glucamide 810
- IUPAC Name:
- Glucamide 810
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Molecular formula (if other than submission substance): C15H31NO6 (C8-Glucamide), C17H35NO6 (C10-Glucamide)
- Molecular weight (if other than submission substance): C8-Glucamide: 321.42 g/Mol, C10-Glucamide: 349.47 g/Mol
- Physical state: Coulourless powder
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Respective amount directly weighed into each test vessel
- Eluate: Synthetic waste water acc. to OECD 209
- Differential loading: Extended limit test to determine the NOEC: 500 - 1000 mg/L
- Controls: test medium without test or reference item; two replicates at the beginning of the test + four replicates at the end of the test
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Culture: Non adapted activated sludge from the sewage plant at Hildesheim (Municipal sewage treatment plant of 31137 Hildesheim, Germany)
- Pretreatment: The sludge was washed twice with chlorine free tap water. An adjustment to a dry sludge concentration of 3.0 g/L ± 10 % was not necessary (actual dry sludge concentration: 3.00 g/L).
The sludge was used within 24 h after sampling.
- Dry sludge concentration: 3.00 g/L corresponding to 1.50 g/L in the test vessel
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- Not determined
- Test temperature:
- 21.6 °C
- pH:
- pH-value of the activated sludge 7.24
pH-value of the synthetic waste water 7.31 - Salinity:
- Not determined
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 mL Erlenmeyer flask, DIN 12380
- Aeration: Permanent; shaking of flasks at 150 rpm
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 2 at test start, 4 at test end
- Composition of test medium:
Test item concentration: the respective amount directly weighed into each test vessel
Test item concentration [mg/L]
500 1000
Inoculum [mL] 250
Synthetic waste water [mL] 16
Demineralised water [mL] 234
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After 3 h incubation, the oxygen concentration of each control, reference item and test item replicate was determined by means of oximeter
immediately after transferring the solution to the measuring cell. The oxygen depletion was recorded for at least 3 minutes on a flat bed recorder.
TEST CONCENTRATIONS
- Range finding study:
Inhibitions were as follows:
Nominal Test Item Concentration [mg/L] pH (test item in demin. water) pH (adjusted to) pH (test item in medium) Inhibition [%]
10 6.21 - 7.47 -9
6.13 - 7.44 -10
100 6.88 - 7.41 -6
6.66 - 7.46 -9
1000 8.94 - 7.58 4
8.81 7.12* 7.48 15
*) adjusted with 0.05 mol/L HCl - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50: 97.3 mg/L - Reported statistics and error estimates:
- The NOEC was determined by calculation of statistical significance of the inhibition of respiration in comparison to the control. One Way Analysis of Variance
(ANOVA) was used for NOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-values
for both Normality and Equal Variance test are 0.05. The -value (acceptable probability of incorrectly concluding that there is a difference) is =0.05.
The EC50-value of the reference item was calculated by linear regression using software GraphPad Prism. Calculation of the confidence intervals for the EC-values was carried out using standard procedures.
Any other information on results incl. tables
From the oxygen depletion the oxygen uptake rates were calculated in mg O2/L×h, additionally the specific respiration rates based on the dry sludge concentration were calculated in mg O2/g×h.
The inhibition of the oxygen uptake rates of the test and reference item concentrations in % was calculated in comparison with the oxygen uptake rates of the control.
Raw Data and Oxygen Uptake Rates of the Control, the Test Item and the Reference Item
Test / Reference Item |
Repl. |
Oxygen Concentration* |
Measured distance** |
Oxygen uptake |
|
Control |
1 |
5.64 |
64 |
25.6 |
|
2 |
5.01 |
64 |
25.6 |
||
3 |
5.54 |
55 |
22.0 |
||
4 |
5.02 |
54 |
21.6 |
||
5 |
5.09 |
56 |
22.4 |
||
6 |
5.06 |
55 |
22.0 |
||
Test item |
500 |
1 |
5.19 |
57 |
22.8 |
2 |
5.15 |
54 |
21.6 |
||
3 |
5.13 |
54 |
21.6 |
||
4 |
6.24 |
59 |
23.6 |
||
5 |
5.24 |
55 |
22.0 |
||
1000 |
1 |
6.72 |
45 |
18.0 |
|
2 |
7.10 |
47 |
18.8 |
||
3 |
6.77 |
45 |
18.0 |
||
4 |
5.02 |
44 |
17.6 |
||
5 |
6.81 |
37 |
14.8 |
||
Reference item |
58 |
1 |
6.02 |
43 |
17.2 |
2 |
6.20 |
43 |
17.2 |
||
3 |
5.98 |
43 |
17.2 |
||
100 |
1 |
6.78 |
30 |
12.0 |
|
2 |
6.72 |
28 |
11.2 |
||
3 |
6.92 |
28 |
11.2 |
||
180 |
1 |
7.81 |
15 |
6.0 |
|
2 |
7.92 |
11 |
4.4 |
||
3 |
7.86 |
10 |
4.0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of the test item C8/C10-Glucamide AC93/13 is 500 mg/L, the EC50 of the test item is > 1000 mg/L.
The test item is not toxic at the concentration of 500 mg/L to activated sludge of a municipal sewage treatment plant. - Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item C8/C10-Glucamide AC93/13 (batch number: AC93/13) from2013-10-31 to 2013-11-06, with the definitive experimental phase on2013-11-06 at the Dr.U.Noack-Laboratorien, 31157Sarstedt, Germany. Test system was activated sludge of the municipal sewage treatment plant of 31137 Hildesheim, Germany. The study was carried out under static conditions with the test item concentrations of 500 and 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates were 4 % and 25 % .
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 97.3 mg/L.
NOEC and EC-ValueofC8/C10-Glucamide AC93/13
EC-value
[mg/L]NOEC*
500
EC50
> 1000
*) No statistically significant effect (p < 0.05), ANOVA, Dunnett’s Method
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