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EC number: 619-880-8 | CAS number: 176972-62-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation
- Remarks:
- in vitro
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- December 3, 1997 to March 13, 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Good Laboratory Practice, The United Kingdom Compliance Programme, Department of Health 1989 and subsequently the United Kingdom Good Laboratory Practice Regulations 1997, Statutory Instrument No. 654. EC Council Directive, 87/18 EEC of 18 December 1986, (No. L 15/29). Good Laboratory Practice in the testing of Chemicals OECD, ISBN 92-64-12367-9, Paris 1982, subsequently republished OECD Environment Monograph No. 45, 1992.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Version / remarks:
- EC Council Directive, 87/18 EEC of 18 December 1986, (No. L 15/29). Good Laboratory Practice in the testing of Chemicals OECD, ISBN 92-64-12367-9, Paris 1982, subsequently republished OECD Environment Monograph No. 45, 1992.
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Chlorohydrine
- IUPAC Name:
- Chlorohydrine
- Reference substance name:
- (l5,25)-(1-benzyl-3-chloro-2-hydroxy-propyl)- carbamic acid methyl ester
- IUPAC Name:
- (l5,25)-(1-benzyl-3-chloro-2-hydroxy-propyl)- carbamic acid methyl ester
- Details on test material:
- Identity: Chlorohydrine
Chemical name: (l5,25)-(1-benzyl-3-chloro-2-hydroxy-propyl)- carbamic acid methyl ester
Intended use: Chemical intermediate
Appearance: White powder
Storage conditions: ca. 4°C in the dark
Expiry: Assumed to be stable for six months from receipt
Date received: 17 July 1997
Constituent 1
Constituent 2
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
Study design: in vivo (LLNA)
- Vehicle:
- other: absolute alcohol
- Concentration:
- 5, 10 and 20% w/w in alcohol
- No. of animals per dose:
- 4 mice
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: na, 0.8, 0.7 and 0.9
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: see Remark
- Remarks:
- TABLE 1 Group dpm/node and test/control ratios Group Concentration dpm number of dpm/node test/control + = positive % w/w lymph nodes ratio - = negative 1 Control 4523 8 565 na na 2 5 3469 8 434 0.8 - 3 10 3029 8 379 0.77 - 4 20 4276 8 535 0.9 - Vehicle = absolute alcohol Test/control ratio of 3 or greater indicates a positive result na Not applicable
Any other information on results incl. tables
In this assay the test/control ratios obtained for 5, 10 and 20% concentrations applied were 0.8, 0.7 and 0.9 respectively which indicates that Chlorohydrine did not show the potential to induce skin sensitisation (delayed contact hypersensitivity).
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Remarks:
- Migrated information
- Conclusions:
- Under the test conditions, Chlorohydrine was not considered a skin sensitizer.
- Executive summary:
The study was performed to assess the skin sensitisation potential of Chlorohydrine using the murine local lymph node assay (LLNA). The assay can be used as a first stage assessment of skin sensitisation potential as indicated in the OECD Guideline for Testing of Chemicals No. 406 "Skin Sensitisation". Adopted 17 July 1992. The assay has undergone validation and has been shown to reliably detect substances that are moderate to strong sensitisers.
The method followed was that described in KIMBER I, HILTON J and WEISENBERGER C (1989) The murine local lymph node assay for identification of contact allergens: A preliminary evaluation of in situ measurement of lymphocyte proliferation Contact Dermatitis 21 215-220 and BASKETTER D.A. and SCHOLES E.W. (1992) Comparison of the local lymph node assay with the guinea-pig maximisation test for the detection of a range of contact allergens. Food and Chemical Toxicology 30, 65-69.
Groups of four mice were used in this study at three dosage levels and a vehicle control. Dosages were selected as follows: 5, 10 and 20% w/w in absolute alcohol
Each group of mice was treated by daily application of 25 µl of each of one of these three concentrations, and vehicle control, to the dorsal surface of both ears for three consecutive days.
The proliferative response of the lymph node cells (LNC) from the draining auricular lymph nodes was assessed five days following the initial application, by measurement of the incorporation or 3H-Thymidine by beta-scintillation counting of LNC suspensions. The response was expressed as radioactive disintegrations per minute per lymph node (dpm/node) and as the ratio of 3HTdR incorporation into LNC of test nodes relative to that recorded for control nodes (test/control ratio).
The test substance is regarded as a sensitiser if at least one concentration of the chemical results in a three-fold greater increase in 3HTdR incorporation compared to control values. In this assay the test/control ratios obtained for 5, 10 and 20% concentrations applied were 0.8, 0.7 and 0.9 respectively which indicates that Chlorohydrine did not show the potential to induce skin sensitisation (delayed contact hypersensitivity).
Conclusion
Therefore, under the test conditions, Chlorohydrine was not considered a skin sensitizer.
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