Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 231-659-4 | CAS number: 7681-11-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- In this study the effects of test chemical on the growth and metabolite accumulation of Staphylococcus auerus (JKB068) and Escherichia coli (JKE029).
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- other: Staphylococcus auerus (JKB068), Escherichia coli (JKE029)
- Details on inoculum:
- Nutrient agar plates containing various concentrations of test chemical were prepared in quintuplicate. The above mentioned organisms were inoculated by standardized loops.
- Test type:
- not specified
- Water media type:
- not specified
- Total exposure duration:
- 24 h
- Hardness:
- No data
- Test temperature:
- 37 deg C
- pH:
- No data
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- No data
- Details on test conditions:
- No data
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 24 h
- Dose descriptor:
- other: Minimum Inhibitory Concentration (MIC100)
- Effect conc.:
- 358.3 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Staphylococcus auerus
- Duration:
- 24 h
- Dose descriptor:
- other: Minimum Inhibitory Concentration (MIC100)
- Effect conc.:
- 219.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Effect were observed on Escherichia coli (JKE029)
- Details on results:
- MIC100 values were determined as the minimum concentration of test chemical that inhibited the growth of the organism.
- Validity criteria fulfilled:
- no
- Conclusions:
- Minimum Inhibitory Concentration (MIC100) value of test chemical in microorganism (Staphylococcus auerus) in a 24hrs. study on growth effect was determine to be 358.3 ± 17.1 mg/L and Minimum Inhibitory Concentration (MIC100) value of test chemical on microorganism (Escherichia coli) in a 24hrs. study on growth effect was determine to be 219.4 ± 9.6 mg/L.
- Executive summary:
In this study the effects of test chemical on the growth and metabolite accumulation of Staphylococcus auerus (JKB068) and Escherichia coli (JKE029). MIC100 values were determined as the minimum concentration of test chemical that inhibited the growth of the organism. Minimum Inhibitory Concentration (MIC100) value of test chemical in microorganism (Staphylococcus auerus) in a 24hrs. study on growth effect was determine to be 358.3 ± 17.1 mg/L and Minimum Inhibitory Concentration (MIC100) value of test chemical on microorganism (Escherichia coli) in a 24 hrs study on growth effect was determine to be 219.4 ± 9.6 mg/L.
Reference
Description of key information
In this study the effects of test chemical on the growth and metabolite accumulation of Staphylococcus auerus (JKB068) and Escherichia coli (JKE029). MIC100 values were determined as the minimum concentration of test chemical that inhibited the growth of the organism. Minimum Inhibitory Concentration (MIC100) value of test chemical in microorganism (Staphylococcus auerus) in a 24hrs. study on growth effect was determine to be 358.3 ± 17.1 mg/L and Minimum Inhibitory Concentration (MIC100) value of test chemical on microorganism (Escherichia coli) in a 24 hrs study on growth effect was determine to be 219.4 ± 9.6 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 219.4 mg/L
Additional information
Data available for the test chemicals, study has been reviewed to determine the short term toxicity on growth of microorganisms of the test chemical. The studies are as mentioned below:
In the first study from peer reviewed journal the effects of test chemical on the growth and metabolite accumulation of Staphylococcus auerus (JKB068) and Escherichia coli (JKE029). MIC100 values were determined as the minimum concentration of test chemical that inhibited the growth of the organism. Minimum Inhibitory Concentration (MIC100) value of test chemical in microorganism (Staphylococcus auerus) in a 24hrs. study on growth effect was determine to be 358.3 ± 17.1 mg/L and Minimum Inhibitory Concentration (MIC100) value of test chemical on microorganism (Escherichia coli) in a 24 hrs study on growth effect was determine to be 219.4 ± 9.6 mg/L.
First study was supported by the second study. Aim of this study was to determine the effect of test chemical on the growth of microorganism. Minimum Inhibitory Concentration were checked after the 24 hrs. Nutrient agar plates containing various concentrations of chemical were prepared in quintuplicate. The above mentioned organisms were inoculated by standardized loops. Minimum Inhibitory Concentration (MIC100) value of test chemical on microorganism (Bacillus subitilis) in a 24hrs study on growth effect was determine to be 147.6 ± 10.2mg/L. Thus chemical consider to be nontoxic.
Similar study was conducted to determine the effect of test chemical on the growth and mortality of test organisms Clostridium species. Nutrient agar plates containing various concentrations of test chemical were prepared in quintuplicate. The two Clostridium species that had been isolated from the cultures of N. closterium and P. tricornutum, were inoculated by standardized loops. MIC100 values were determined as the minimum concentration of test chemical that inhibited the growth of the organism. Test conducted for 24 hrs. Inhibition observed at 231.5 mg/l and 200.9 mg/l on 2nd species.
Thus based on the above results, chemical toxicity ranges from 147 mg/l to 231.5 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.