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EC number: 216-223-3 | CAS number: 1530-32-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- as mentioned below
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two toxicity study of microorganism for the test chemical :
1) To evaluate Oxygen consumption of bacterial culture under the influence of test substance
2) Effect of test material was evaluated on Photobacterium sp. by luminescent bacteria test - GLP compliance:
- not specified
- Details on test solutions:
- 1) The stock solution was prepared with Tween 80 as dispersant and was stirred for 17 hours, resulting in a white suspension. The further dilutions were prepared from the stock solution while stirring
- Test organisms (species):
- other: 1) Pseudomonas putida 2)Photobacterium sp.
- Details on inoculum:
- 1) - Strain : DSM 50026
- Test type:
- static
- Water media type:
- other: 1) algar slant
- Total exposure duration:
- 30 min
- Test temperature:
- 1) 25 °C.
- Nominal and measured concentrations:
- 1) 156.25, 312.5, 625, 1250, 2500, 10000 mg/l.
- Details on test conditions:
- 1) - Test vessle: test tube
-MEDIUM: 25 g/l nutrient broth I, 18 g/l Agar poured into slant-agar tubes. - Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 100 000 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- other: Oxygen consumption
- Duration:
- 15 min
- Dose descriptor:
- EC50
- Effect conc.:
- 202 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- other: Bio Luminescence
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The test chemical is not likely to be toxic to microorganism atleast in the dose range of 202- 10000 mg/l
- Executive summary:
Data available for the test chemicals has been reviewed to determine the toxicity of microorganism .The studies are as mentioned below:
1) The effect of test material on Pseudomonas putida was observed for its Oxygen consumption. Pseudomonas putida, strain DSM 50026 were cultured on 25 g/l nutrient broth , 18 g/l Agar poured into slant-agar tubes. The stock solution was prepared with Tween 80 as dispersant and was stirred for 17 hours, resulting in a white suspension. The further dilutions were prepared from the stock solution while stirring. The nominal test concentration used were 156.25, 312.5, 625, 1250, 2500, 10000 mg/l. The effect concentartion EC50 was after 30 min of eposure to test organism Pseudomonas putida was observed to be >10000 mg/l. Based on the above effect concentration it can be considered that test substance is not toxic for the microorganisms.
2) Effect of test material on bio luminescence property of Photobacterium sp. was evaluated after 15 min of exposure of test material. The EC50 value of test material on Photobacterium sp. after 15 mins was observed to be 202 mg/l. Based on the effect concentration it can be concluded that test material has no adverse effect on microorganism.
Reference
Description of key information
Toxicity to microorganism:
Data available for the test chemicals has been reviewed to determine the toxicity of microorganism .The studies are as mentioned below:
1) The effect of test material on Pseudomonas putida was observed for its Oxygen consumption. Pseudomonas putida, strain DSM 50026 were cultured on 25 g/l nutrient broth , 18 g/l Agar poured into slant-agar tubes. The stock solution was prepared with Tween 80 as dispersant and was stirred for 17 hours, resulting in a white suspension. The further dilutions were prepared from the stock solution while stirring. The nominal test concentration used were 156.25, 312.5, 625, 1250, 2500, 10000 mg/l. The effect concentartion EC50 was after 30 min of eposure to test organism Pseudomonas putida was observed to be >10000 mg/l. Based on the above effect concentration it can be considered that test substance is not toxic for the microorganisms.
2) Effect of test material on bio luminescence property of Photobacterium sp. was evaluated after 15 min of exposure of test material. The EC50 value of test material on Photobacterium sp. after 15 mins was observed to be 202 mg/l. Based on the effect concentration it can be concluded that test material has no adverse effect on microorganism.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 202 mg/L
Additional information
Toxicity to microorganism:
Data available for the test chemicals has been reviewed to determine the toxicity of microorganism .The studies are as mentioned below:
1) The effect of test material on Pseudomonas putida was observed for its Oxygen consumption. Pseudomonas putida, strain DSM 50026 were cultured on 25 g/l nutrient broth , 18 g/l Agar poured into slant-agar tubes. The stock solution was prepared with Tween 80 as dispersant and was stirred for 17 hours, resulting in a white suspension. The further dilutions were prepared from the stock solution while stirring. The nominal test concentration used were 156.25, 312.5, 625, 1250, 2500, 10000 mg/l. The effect concentartion EC50 was after 30 min of eposure to test organism Pseudomonas putida was observed to be >10000 mg/l. Based on the above effect concentration it can be considered that test substance is not toxic for the microorganisms.
2) Effect of test material on bio luminescence property of Photobacterium sp. was evaluated after 15 min of exposure of test material. The EC50 value of test material on Photobacterium sp. after 15 mins was observed to be 202 mg/l. Based on the effect concentration it can be concluded that test material has no adverse effect on microorganism.
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