cyclohexyl salicylate

Long-term toxicity to fish

Administrative data

Endpoint:

adult fish: sub(lethal) effects

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1985

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: UBA Guideline – “Sublethal effects on Zebrafish – Brachydanio rerio”

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

400-410-3

EC Name:

-

Cas Number:

25485-88-5

Molecular formula:

C13H16O3

IUPAC Name:

cyclohexyl 2-hydroxybenzoate

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

28 d

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

0.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: symptomatic observations

Effect concentrations 2

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

0.24 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

other: symptomatic observations

Effect concentrations 4

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

0.48 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The sub-chronic toxicity of CYCLOHEXYL SALICYLATE to Zebrafish (Brachydanio rerio) was studied over a 28-Day period under semi-static conditions (renewal every 48- or 72-hours). The study duration was extended from 14-Days to 28-Days to define a more accurate NOEC, since levels of toxicity observed over the initial 14-Day period was considered minor. Test concentrations (0.1 to 4.0 mg/L nominal) and a Control in drinking water were studied. Mortality and a suite of behavioural observations (narcosis, apathy, lack of reactivity, impaired balance, cramp, discolouration, jumpiness/nervousness, hyper-ventilation, reduction in sexual interest, shyness) were noted during the course of the experiment. Substance specific analyses by HPLC were performed to determine the actual water concentrations. Analytics indicated that the detected amounts of CYCLOHEXYL SALICYLATE in freshly prepared medium were the order of 63 to 105% of nominal. This figure decreased to, on average, 35% of nominal after 48 hours, and, 20% of nominal after 72 hours.