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EC number: 220-260-0 | CAS number: 2691-41-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 10 to 31 July 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study run to a reliable method but not GLP and no guideline followed.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Five dose levels were selected in which 5 groups of 5 males and 5 females mice were dosed once. An additional group of 5 male and 5 female mice was dosed with vehicle only. The mice were observed in the morning and afternoon for 14 days following administration of the test substance. At death, or at the end of the observation period and at termination, each animal was subjected to a gross post mortem examination.
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine
- EC Number:
- 220-260-0
- EC Name:
- Octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine
- Cas Number:
- 2691-41-0
- Molecular formula:
- C4H8N8O8
- IUPAC Name:
- 1,3,5,7-tetranitro-1,3,5,7-tetrazocane
- Test material form:
- solid: crystalline
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- B6C3F1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River UK Limited
- Weight at study initiation: The mean body weight on being dosed was 22 g (range 20-22 g) in the dose ranging study for males and 21 g (range 19-22 g) in the dose ranging study for females. The mean body weight in the main study was 22 g (range 18-24 g) for males and 18.5 g (range 16-21 g) for females.
- Fasting period before study: The animals were deprived of food for a 4 h period prior to dosing.
- Housing: They were housed individually in polypropylene cages with stainless steel grid tops and sterilised wood shavings.
- Diet (e.g. ad libitum): The animals were fed on BP Nutrition expanded Mouse Maintenance Diet No. 1.
- Water (e.g. ad libitum): Water was available ad libitum throughout the study
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22°C (extremes of 19°C-25°C)
- Humidity (%): 57% (extremes of 52%-74%)
IN-LIFE DATES: From 10 to 31 July 1980
No additional data
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Remarks:
- 0.5% low viscosity CMC
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 20 mL/kg
MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg
No additional data - Doses:
- Dose ranging study:
300, 700, 1500, 5000 and 15000 mg wet test substance/kg
247, 577, 1236, 4120 and 12360 mg dry test substance/kg
Main study:
1200, 2040, 3468, 5895.6 and 10022.5 mg wet test substance/kg
956, 1626, 2764, 4699 and 7988 mg dry test substance/kg - No. of animals per sex per dose:
- Dose ranging study: one male and one female
Main study: 5 males and 5 females - Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: The mice were observed on the morning and afternoon for 14 days following administration of the test substance.
- Necropsy of survivors performed: yes, At death, or at the end of the observation period and sacrifice, each animal was subjected to a gross post mortem examination.
- Other examinations performed: clinical signs, gross pathology - Statistics:
- The LD50 was calculated using a method based on the following:
Finney (1971) "Probit Analysis", Cambridge University Press.
Results and discussion
- Preliminary study:
- In the dose ranging study in pairs of mice, mortality was 0/2, 0/2, 1/2, 1/2 and 1/2 in the dose group 247, 577, 1236, 4120 and 12360 mg dry test substance/day dose groups, respectively.
Clinical signs included piloerection, hypokinesia and ataxia, lasting for up to 3 days after dosing.
Post mortem observations included white fluid in stomach and upper gastro-intestinal tract with kidneys pale and mottled.
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 1 670 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 440 - <= 1 890
- Remarks on result:
- other: Based on dry test substance
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 3 240 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 2 920 - <= 3 570
- Remarks on result:
- other: Based on dry test substance
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 300 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 2 110 - <= 2 500
- Remarks on result:
- other: Based on dry test substance
- Mortality:
- In the main study, mortality was 0/10, 4/10, 5/10, 10/10 and 10/10 in the 956, 1626, 2764, 4699 and 7988 mg dry test substance/kg dose groups, respectively.
- Clinical signs:
- other: Clinical signs included piloerection, soiled coat, hyperkinesia, hypokinesia, ataxia, sedation, eyes half shut and penis protruded. In one animal the penis was very swollen and stained with blood with a constant stream of urine.
- Gross pathology:
- Post mortem observations included stomach and gastro-intestinal tract filled with white fluid, gut contents fluid, blood filled gut, stomach wall white, penis extended and dark red and lungs red.
- Other findings:
- No information provided
Any other information on results incl. tables
There were no deaths or clinical signs recorded in the control group.
No abnormalities were detected at post mortem observations in the control group.
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The Oral Median Lethal Doses (LD50s) of dry test substance were calculated to be 1960 mg/kg for male, 3240 mg/kg for female and 2300 mg/kg for male and female B6C3F1 mice.
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