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EC number: 205-201-9 | CAS number: 135-57-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The test was based on OECD Guideline for Testing of Chemicals [No.471. Bacterial Reverse Mutation Test(Ames test), July. 1997]. This final report and the original raw data were reviewed for adherence to ISO/IEC17025: 2005 General Requirements for the competence of Testing Calibration Laboratories. OECD Guideline for Testing of Chemicals (No.471. Bacterial Reverse Mutation Test(Ames test), July. 1997);State Environmental Protection Administration : The Guidelines for the Testing of Chemicals ; Ministry of Health, 2005: Technical standards for testing of chemicals .
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- N,N'-dithiodi-o-phenylenedibenzamide
- EC Number:
- 205-201-9
- EC Name:
- N,N'-dithiodi-o-phenylenedibenzamide
- Cas Number:
- 135-57-9
- Molecular formula:
- C26H20N2O2S2
- IUPAC Name:
- N-{2-[(2-benzamidophenyl)disulfanyl]phenyl}benzamide
Constituent 1
Method
- Target gene:
- Histidine requiring strains of salmonella typhimurium TA97, TA98, TA100 and TA102.
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 97
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- 0.5ml of S9-mixture. The used active system is a cofactor-supplemented post-mitochondrial fraction (S9-mix). The used concentrations of S9-mix are 18.5%(v/v) in final concentration.
- Test concentrations with justification for top dose:
- According to the preliminary experiment,which indicated that antibacterial effects were found to the tested bacteria at the concentrations of 5000 and 2500 μg/plate, this study set four lower concentrations for the tested substance, the final concentrations were 1250, 625, 312 and 156 μg/plate separately.
Controlsopen allclose all
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- CAS: 26628-22-8
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminofluorene
- Remarks:
- CAS: 153-78-6
- Positive control substance:
- other: fenaminosulf
- Remarks:
- CAS: 140-56-7
- Positive controls:
- yes
- Positive control substance:
- mitomycin C
- Remarks:
- CAS: 50-07-07
- Positive controls:
- yes
- Positive control substance:
- other: 1,8-dihydroxyanthraquinone
- Remarks:
- CAS: 117-10-2
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
__
Results(X ±SD)
Table 1
Groups |
Dosage* (μg/plate) |
With or not withS9 |
TA97 |
TA98 |
TA100 |
TA102 |
Spontaneous reverse mutation |
- |
Yes |
140±12 |
35±3 |
154±11 |
263±14 |
No |
153±13 |
37±3 |
181±15 |
291±23 |
||
Solvent control |
- |
Yes |
157±9 |
36±4 |
152±12 |
280±18 |
No |
163±16 |
40±2 |
169±7 |
287±9 |
||
Test substance |
1250 |
Yes |
142±11 |
33±3 |
115±4 |
164±16 |
No |
147±7 |
38±2 |
132±8 |
136±14 |
||
625 |
Yes |
146±12 |
39±4 |
127±8 |
212±5 |
|
No |
141±15 |
36±2 |
145±11 |
157±11 |
||
312 |
Yes |
137±5 |
38±5 |
129±5 |
201±10 |
|
No |
155±6 |
39±2 |
156±15 |
141±19 |
||
156 |
Yes |
144±13 |
37±2 |
151±4 |
172±13 |
|
No |
151±13 |
35±1 |
140±6 |
138±13 |
||
Positive controls |
Sodium azide (1.5μg/plate) |
Yes |
- |
- |
2286±115 |
- |
No |
- |
- |
- |
- |
||
2-Aminofluorene (10.0μg/plate) |
Yes |
- |
- |
- |
- |
|
No |
2030±88 |
1973±96 |
1758±124 |
- |
||
Fenaminosulf (50.0μg/plate) |
Yes |
1838±114 |
1764±98 |
- |
- |
|
No |
- |
- |
- |
- |
||
Mitomycin C (4.0μg/plate) |
Yes |
- |
- |
- |
1848±136 |
|
No |
- |
- |
- |
- |
||
1,8-Dihydroxyanthraquinone (50.0μg/plate) |
Yes |
- |
- |
- |
- |
|
No |
- |
- |
- |
1070±104 |
Note: *:According to the preliminary experiment,which indicated that antibacterial effects were found to the tested bacteria at the concentrations of 5000 and 2500μg/plate, this study set four lower concentrations for the tested substance.- means not available.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
pepton 22 has been shown to be not mutagenic
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