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Diss Factsheets
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EC number: 935-560-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- In accordance with ISO/IEC 17025
Test material
- Test material form:
- liquid
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Not adapted and not pre-conditioned activated sludge from the aeration tank of the ARA Werdhölzli (CH-8048 Zürich), a municipal biological waste water treatment plant. 1.5 g/l dry matter in the final mixture.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- 36.6-37.5 (total hardness)
- Test temperature:
- 22 ± 2 °C
- pH:
- 7.2-7.4
- Conductivity:
- 691-731 uS/cm (20ºC)
- Nominal and measured concentrations:
- The nominal concentrations of SPRPUZP were 125, 250, 500, 1000 and 2000 mg/l, respectively. These test concentrations were prepared by direct addition of the test substance.
No analysis of test concentrations was conducted. For the calculation of the EC10, EC20, EC50 and EC80 values the nominal content of the test substance in the test media was used (calculations based on assumption that test material consists of 100% a.i.). - Details on test conditions:
- Test units: 1000 ml flasks containing a total volume of test suspension of 500 ml; aerated by shaking (150 r.p.m.)
Measuring apparatus: 300 ml flat bottom BOD flasks; polarographic oxygen electrode, connected to a potentio-metric recorder - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 193 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 325 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 572 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Not subject to classification and labelling.
- Executive summary:
The inhibitory effect of SPRPUZP on the oxygen consumption of activated sludge microorganisms of a municipal sewage treatment plant was investigated under aerobic static conditions. The inhibitory effect may include the effect on respiration and nitrification. The exposure time of the test substance on the activated sludge was 3 h.
The nominal concentrations of SPRPUZP were 125, 250, 500, 1000 and 2000 mg/l, respectively. These test concentrations were prepared by corresponding dilution of stock solutions of the test substance.
No chemical analysis of test concentrations was conducted. For the calculation of the effective concentration leading to 10, 20, 50, and 80% respiration inhibition (EC10, EC20, EC50, EC80) the nominal content of the test substance was used, assuming the test compound to be stable in water over 3 h.
The following respiration inhibition of the activated sludge compared to the two blank controls was observed:
2000 mg/l (60%), 1000 mg/l (26%), 500 mg/l (46%). No significant toxic effects (<10% respiration inhibition) were observed at 250 and 125 mg/l.
Based on the inhibition data the EC10 value of SPRPUZP was calculated to be 193 mg/l, the EC20 value 325 mg/l and the EC50 value 1572 mg/l (95% confidence interval not assessable). The EC80 value was estimated to be >2000 mg/l.
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