Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 471-510-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006-07-03 to 2006-08-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- adopted 17 July 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- 31 July 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 471-510-2
- EC Name:
- -
- Cas Number:
- 872182-46-2
- Molecular formula:
- C28H46O7
- IUPAC Name:
- 1,3-bis(2-ethylhexyl) 2-[(4-hydroxy-3,5-dimethoxyphenyl)methyl]propanedioate
- Test material form:
- liquid: viscous
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- The inoculum activated sludge, microorganisms from a domestic waste water treatment plant was supplied by the sewage plant Rossdorf, Germany.
The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 1.5 g dry material per litre were mixed with test water and then aerated until use. The washed activated sludge was aerated overnight and used the following day for the experiment. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- ca. 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Preparation of Test Flasks:
The amounts of test item and reference item were directly weighed into the test flasks. No emulsifiers or solvents were used, but the solutions were dispersed by stirring to achieve a homogeneous solution of the test item.
Incubation:
The closed test flasks were incubated in a climatic chamber under continuous stirring. The consumption of oxygen was determined by measuring the change of pressure in the flasks. Evolved carbon dioxide was absorbed in an aqueous solution (45 %) of potassium hydroxide.
Test Duration: 28 days
Measurement of Oxygen:
The change of pressure in the test flasks was measured by means of a manometric method (BSBBOD-Sensor-System, Aqualytic, 63231 Neu Isenburg, Germany) each day.
Temperature: Temperature was measured each working day in the climatic chamber.
pH Value: pH-values were measured in all flasks at the start and end of the test using a pH-electrode WTW pH 340i.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 26
- Sampling time:
- 28 d
- Details on results:
- The reference item sodium benzoate was sufficiently degraded to 99 % after 14 days, and to 105 % after 28 days of incubation.
The percentage biodegradation of the reference item confirms the suitability of the used activated sludge inoculum.
In the toxicity control containing both, the test item and the reference item sodium benzoate, 40 % biodegradation was noted within 14 days and 45 % biodegradation was determined after 28 days of incubation.
The oxygen demand in the abiotic control was 0 mg/L.
The test item was degraded to 26 % after 28 days of incubation.
The degradation rate of the test item did not reach 60 % within the 10-day window and after 28 days of incubation. Therefore, the test item is considered not to be readily biodegradable.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- In a Manometric Respirometry Test performed according to OECD 301F and under GLP regulation, the substance was degraded to 26% after 28 days of incubation. The degradation rate of the substance substance did not reach 60% within the 10 -day window and after 28 days of incubation. Therefore, the substance is considered not to be readily biodegradable.
- Executive summary:
Purpose
The purpose of this assay was to provide information on the biodegradability of the test item in aqueous environment and thus serve as a rational basis for risk assessment for environmental fate in aqueous compartments.
Study Design
For this purpose, the test item was exposed to activated sludge from the aeration tank of a domestic waste water treatment plant for 28 days. The biodegradation was followed by the oxygen uptake of the microorganismsduring exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. This study is recognized by the international test guidelines and should provide a rational basis to assess the ready biodegradation properties of the test item when incubated with activated sludge.
Results
Biodegradation
The test item did not reach 60 % within the 10-day window and after 28 days of incubation the test item was degraded to 26 %.
Biodegradation of sodium benzoate
The reference item sodium benzoate was sufficiently degraded to 99 % after 14 days, and to 105 % after 28 days of incubation (ThODNH4).
Biodegradation in the Toxicity Control
In the toxicity control containing both, the test item and the reference item sodium benzoate, 40 % biodegradation was noted within 14 days and 45 % biodegradation was determined after 28 days of incubation.
Oxygen demand of the abiotic control
The oxygen demand in the abiotic control was 0 mg/L.
Conclusion
The degradation rate of the test item did not reach 60 % within the 10-day window and after 28 days of incubation. Therefore, the test item is considered not to be readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.