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EC number: 617-251-2 | CAS number: 816431-72-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 May - 13 Jun 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- HPLC-UV/VIS
- Details on sampling:
- - Concentrations: 0 and 100 mg/L
- Sampling method: Aliquots of the samples from the biological test were directly analysed by HPLC
- Sample storage conditions before analysis: Routinely, the samples are analysed immediately. Only in exceptional cases, they are stored overnight deeply frozen and protected from light. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To produce the only test concentration 105.3 mg of the test item was added to 1 L of dilution water, then treated with an ultrasonic bath for 1 h and afterwards stirred for 24 h on a magnetic stirrer. Finally undissolved particles of the test item were removed by filtration using a folded filter of pore size 7-12 µm.
- Controls: Yes
- Test concentration separation factor: Single concentration was used
- Evidence of undissolved material: undissolved particles of the test item were removed by filtration - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain/clone: Strain of Bundesgesundheitsamt Berlin
- Stage and instar at study initiation: Neonates
- Method of breeding: Parthenogenesis (females)
- Source: Bundesgesundheitsamt Berlin
- Age of parental stock: maintained since more than 15 years in the test facility
- Feeding during test: no feeding during the exposure period - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 15.7 °dH (= 280.25 mg/L CaCO3)
- Test temperature:
- 21.1 - 21.3 °C
- pH:
- 7.8 - 8.2
- Dissolved oxygen:
- 96 - 97 %
- Nominal and measured concentrations:
- Nominal = 100 mg/L
Measured = 96.098 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL glass beakers
- Volume of solution: 20 mL
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration: 2
- No. of vessels per control: 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (so-called 'M4 medium', originally described in Water Research 24 (9), September 1990: 1157-1167), prepared according to the recommendations of Bundesgesundheitsamt Berlin.
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light :8hdark
EFFECT PARAMETERS MEASURED:
Induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 h - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Other biological observations: none
- Mortality of control: 0 %
- Immobilisation of control: 0 %- Validity criteria fulfilled:
- yes
- Remarks:
- See more details in the table in the section "Any other information on results incl. tables"
- Conclusions:
- No toxic effects against daphnia at a concentration of 100 mg/L; 48h EC50 > 100 mg/L (nominal concentration) were observed.
- Executive summary:
The 48 h-acute toxicity of the test substance to Daphnia magna was studied under static conditions in a limit test. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (OJ. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia'. Test species were exposed to control and test chemical at nominal concentrations of 0 and 100 mg/L for 48 hr. Immobilisation and locomotion effects were monitored daily and no toxic effects were observed at the limit concentration of 100 mg/L. Therefore the 48 h EC50 was >100 mg/L. The results are expressed in terms of nominal concentrations. The test substance is an acid chloride which easily hydrolyses to the corresponding acid in aqueous media. Therefore the acid was analysed by HPLC. Recovery rates correspond to 96 % of nominal values at 0 hours and to 96.1% of nominal values at 48 hours, respectively.
Reference
Validity criteria for the measurement of the daphnia toxicity
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
pH should not vary by more than 1 unit | pH range was 7.8 to 8.2 | Yes |
In the control, including the solubilising agent, not more than 10 % of the daphnids should have been immobilized. (Not more than 10 % of the control daphnids should show immobilisation or other signs of disease or stress, for example, discoloration or unusual behaviour such as trapping at surface of water) | No immobilization observed in control | Yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/l in control and test vessels. | Dissolved oxygen concentration was atleast 8.5 mg/L in control and test vessels. | Yes |
A limit test may be performed at 100 mg/L of the test substance in order to demonstrate that the EC50 is greater than this concentration. The limit test should be performed using 20 daphnids, with the same number in the control. If the percentage of immobilisation exceeds 10% at the end of the test, a full study chould be conducted. | Within the limit test, 20 daphnids were used for the test concentration of 100 mg/L (2 replicates and 10 daphnia per test vessel) and 20 daphnids for the control (2 replicates and 10 daphnia per test vessel). No immobilization of daphnids was observed within the test. | Yes |
The concentration of the test substance shall be maintained to within 80 % of the initial concentration throughout the course of the test. | Recovery rates correspond to 96.0 % of nominal values at 0 h, and to 96.1 % of nominal values at 48 h, respectively | Yes |
Description of key information
A 48 h-acute toxicity of the test substance to Daphnia magna was studied under static conditions in a limit test. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (OJ. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia'. Test species were exposed to control and test chemical at nominal concentrations of 0 and 100 mg/L for 48 hr. Immobilisation and locomotion effects were monitored daily and no toxic effects were observed at the limit concentration of 100 mg/L. Therefore the 48 h EC50 was >100 mg/L. The results are expressed in terms of nominal concentrations. The test substance is an acid chloride which easily hydrolyses to the corresponding acid in aqueous media. Therefore the acid was analysed by HPLC. Recovery rates correspond to 96 % of nominal values at 0 hours and to 96.1% of nominal values at 48 hours, respectively.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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