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EC number: 427-820-5 | CAS number: 2701-50-0 1,2-METHYLEN-4,6-DIENACETAT
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16. Apr - 17. Aug 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412, L8 (Pseudomonas - Zellvermehrungshemmtest)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- nutrient solutions:
Solution 1: 10.0 g sodium nitrate, 2.4 g dipotassium hydrogen phosphate, 1.2 g potassium dihydrogenphosphate, 1.0 g yeast extract added to 500 mL distilled water
Solution 2: same as solution 1, excluding yeast extract
Solution 3: 40 g D (+) glucose-monohydrate added to 500 ml distilled water
Solution 4: 4 g magnesiumsulphate-heptahydrate, 0.01 g iron citrate, added to 1,000 mL distilled water
Stock solution:
- 112.5 mg test substance dissolved in 1000 mL double distilled water.
- Stirred for 24 hours at room temperature
- This solution was used for the highest concentration.
- Subsequently, this stock solution was filtered.
Test solutions:
- Test concentration separation factor: 2
- 80 mL of the respective solutions were poured into the test vessels.
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: Pseudomonas putida
- Name and location of sewage treatment plant where inoculum was collected: "Deutschen Sammlung für Mikroorganismen, Braunschweig"
- Method of cultivation: Cultivated on slant agar containing a nutrient solution at approximately 25 °C. During culturing the microorganisms were transferred weekly
- Preparation of inoculum for exposure:
The microorganisms for preculture:
- From seven-day-old stock
- approximately 7 hours before the start of incubation.
Pre-culture nutrient solution:
- 25 ml of solution 1, 25 ml of solution 3 and 50 ml of solution 4 (see details on test solutions) in 900 ml of sterile distilled water.
- The preculture had a turbidity of 0.07 absorbance units (corresponding to approximately TE/F 10, spectrophotometer)
-Subsequently incubated for 7 hours at room temperature (0.437 absorbance units) -Dilution of the preculture to 0.229 absorbance units (equivalent to approximately TE/F 50). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 16 h
- Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2 (blank control)
Preparation of the test solutions and addition of the inoculum:
- 2.5 mL of nutrient solution 2, 2.5 mL of nutrient solution 3, and 5 mL of nutrient solution 4 were added to the 80 mL of test compound solutions and 10 mL of the inoculum. - Reference substance (positive control):
- no
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- not determinable
- Remarks:
- The test substance produced no inhibitory effects as a saturated aqueous solution.
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The slight reduction in growth was not concentration dependent and is not considered relevant. No inhibitory effect of the test substance on the growth of Pseudomonas putida up to the solubility limit of about 1 mg/L was observed. Therefore, the test solution is not toxic to Pseudomonas putida up to the solubility limit.
- Executive summary:
The study was perfomred to assess the toxicity of the test substance to bacteria. The study was conducted in accordance with the DIN 38412 L8. The slight reduction in growth was not concentration dependent and is not considered relevant. No inhibitory effect of the test substance on the growth of Pseudomonas putida up to the solubility limit of about 1 mg/L (according to acute toxicity to fish, Länge, 1998) was observed. Therefore, the test solution is not toxic to Pseudomonas putida up to the solubility limit.
Reference
Measurement of the turbidity:
The turbidity of the test concentrations, the control and the test solutions without inoculum were measured after 16 hours. All measurements were performed at a wavelength of 436 nm (Spectral photometer) and the absorbance was determined in TE/F units according to DIN 38404.
Inhibition percentage was calculated according to the following formula:
inhibition [%] = ((TE/F control [16 h] - TE/F test concentration [16 h]) / TE/F control [16 h]) *100
Result:
Turbidity and grawth inhibition of the test and contral solutions after 16 hours
Concentration (Stock solution, saturated) | TE/F +/- Std. div. | growth inhibition [%] |
Control | 364 +/- 24 | 0 |
1:8 diluted, filtered | 369 +/- 18 | -1.37 |
1:4 diluted, filtered | 391 +/- 9 | -7.42 |
1:2 diluted, filtered | 389 +/- 12 | -6.87 |
not diluted, filtered | 384 +/- 3 | -5.49 |
not diluted, not filtered | 385 +/- 19 | -5.77 |
The turbidity of the contral at 0 hours as weil as of all blank solutions after 16 hours was < 1 TE/F. The parallel test vessels showed no relevant variation.
Description of key information
The study was performed to assess the toxicity of the test substance to bacteria. The study was conducted in accordance with the DIN 38412 L8. The slight reduction in growth was not concentration dependent and is not considered relevant. No inhibitory effect of the test substance on the growth of Pseudomonas putida up to the solubility limit of about 1 mg/L (according to acute toxicity to fish, Länge, 1998) was observed. Therefore, the test solution is not toxic to Pseudomonas putida up to the solubility limit.
Key value for chemical safety assessment
Additional information
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