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EC number: 606-682-1 | CAS number: 2098-65-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- effects on growth of green algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Oct 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 17-Hydroxy-1 alpha,2 alpha-methylene-4,6-pregnadiene-3,20-dione
- EC Number:
- 606-682-1
- Cas Number:
- 2098-65-9
- Molecular formula:
- C22 H28 O3
- IUPAC Name:
- 17-Hydroxy-1 alpha,2 alpha-methylene-4,6-pregnadiene-3,20-dione
- Test material form:
- solid: bulk
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- TOC measurement
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: For the preparation of the test solutions a suspension with a nominal loading of 100 mg ZK 12126 per liter of demineralized water was stirred for approximately 26 hours. This suspension was filtered through a glassfibre filter. The resulting solution served as the highest concentration (saturated solution). It was further diluted 1:2, 1:4, 1:8 and 1:16 with demineralized water.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Source (laboratory, culture collection): Sammlung Algenkulturen Göttingen
- Age of inoculum (at test initiation): 3 hours
ACCLIMATION
- Culturing media and conditions (same as test or not): same as test
- Any deformed or abnormal cells observed: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 24 °C
- pH:
- start: 8.0 - 8.1
end: 8.7 - 9.2 - Nominal and measured concentrations:
- Nominal : 100 mg/L
measured: 17.5 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass bottles
- Control end cells density: 988000 cells/mL
- No. of organisms per vessel: 10000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: The cell concentration of the test solutions and of the control were measured after 24, 48 and 72 hours of incubation. All measurements were made on an electronic particle counting apparatus (Coulter Counter), which was calibrated with a count of standard cell concentrations using the "Bürker'-counting chamber.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 17.5 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 17.5 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
- Unusual cell shape: no
Any other information on results incl. tables
Table 1: Inhibition of the growth rate (%) of Scenedesmus subspicatus after 72 hours exposure to 1,2 Methylen-4,6 -Dien
extrapolated concentration | Inhibition in percent | |
of ZK 12126 (mg/I) | Biomass (integral) | Growth rate |
0,0 (control) | 0 | 0 |
1,1 | -19,5 | 0 |
2,2 | -16,5 | 0,2 |
4,4 | -58,5 | -10 |
8,8 | -86,4 | -14,3 |
17,5 | 63,1 | 23,1 |
In a range-finding study 90% inhibition was observed in the saturated solution
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 1,2 Methylen-4,6-Dien had an inhibitory effect on the growth of Scenedesmus subspicatus at a concentration of 17.5 mg/L (saturated solution)
while in lower concentrations a slight increase of the algae may have occurred. It can be concluded that ZK 12126 is toxic to the alga S. subspicatus close to the solubility limit. The EbC5O (biomass) and also the ErC5O (growth rate) could
not be calculated due to the effect pattern . - Executive summary:
The purpose of this study was to determine the effects of the test compound 1,2 Methylen-4,6-Dien on the growth of the green algae Scenedesmus subspicatus. The study was conducted in agreement with the OECD guideline no. 201.
The test substance was incubated in an aqueous solution including nutrients with an algae population of Scenedesmus subspicatus for a test duration of approximately 70 hours. The nutrient solution was made up of mainly nitrate, phosphates and some trace elements. For the preparation of the test solutions a suspension with a nominal loading of 100 mg test substance per liter of demineralized water was stirred for approximately 26 hours. The resulting solution served as the highest concentration (saturated solution). It was further diluted 1 :2, 1 :4, 1:8 and 1 :16 with demineralized water. The organic carbon concentration of the test solution was analysed with a TOC analyser at the start of the incubation. The substance concentration was calculated on the basis of the molecular formula. The concentration was 17.5 mg/I. The test concentrations extrapolated are shown in the table below. The algae were exposed to each concentration in triplicate. Six vessels were prepared for the contro!. The algae were incubated in an incubator shaker under continuous light.
Control solutions were counted with an electronic particle counter ("Coulter Counter"). The increase of biomass and the growth rate were calculated on the basis of the cell counts. The calculated biomass and growth rate of each concentration were compared to those of the controls, and the inhibition was calculated.
1,2 Methylen-4,6-Dien had an inhibitory effect on the growth of Scenedesmus subspicatus at a concentration of 17.5 mg/L (saturated solution) while in lower concentrations a slight increase of the algae may have occurred. The EbC5O (biomass) and also the ErC5O (growth rate) could not be calculated due to the effect pattern .
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