Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-823-7 | CAS number: 74-94-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-03-20 to 2017-05-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- adopted July 17, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- EEC Publication No. L 142/496, May 2008
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Aerobic activated sludge, microorganisms from a domestic waste water treatment plant was supplied by the sewage treatment plant Rossdorf, Germany.
- Pre-treatment: The aerobic activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in test water and centrifuged again. This procedure was done three times. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined.
- Concentration of sludge: 3.5 g dry material per litre were mixed with test water - Duration of test (contact time):
- 28 d
- Initial conc.:
- 103.7 mg/L
- Based on:
- test mat.
- Remarks:
- Test Item Loading Rate
- Initial conc.:
- 281.6 mg/L
- Based on:
- ThOD
- Remarks:
- THOD NH4
- Initial conc.:
- 394.3 mg/L
- Based on:
- ThOD
- Remarks:
- THODNO3
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Reconstituted water: Analytical grade salts were added to pure water to prepare the following stock solutions:
a) 8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4 x 2 H2O, 0.5 g NH4Cl filled up with pure water to 1000 mL volume; The pH-value was 7.4.
b) 11.25 g MgSO4 x 7 H2O filled up with pure water to 500 mL volume
c) 18.2 g CaCl2 x 2 H2O filled up with pure water to 500 mL volume
d) 0.125 g FeCl3 x 6 H2O filled up with pure water to 500 mL volume
In order to avoid precipitation of iron hydroxide in the stock solution d), one drop of concentrated HCl per litre was added before storage. 50 mL of stock solution a) and 5 mL of the stock solutions b) to d) were combined and filled up to a final volume of 5000 mL with pure water.
- Test temperature: 22°C ± 1°C
- pH: 7.6 (measured at the start of the test); 7.6 to 7.9 (measured at the end of the test)
- pH adjusted: no
- Aeration of dilution water: No
- Suspended solids concentration: 3.5 g dry material per litre were mixed with test water; final sludge concentration in test flasks: 28.7 mg sludge/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Manometric Test System with test flasks containing a volume of approximately 500 mL. BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany
- Number of culture flasks/concentration: Two replicates for DMAB (103.7 mg/L), two replicates for inoculum control, one replicate for the procedure control, abiotic control, and the toxicity control.
- Preparation of Test flask: The amounts of test item and reference item were directly weighed into the test flasks. No emulsifiers or solvents were used, but the solutions were dispersed by stirring to achieve a homogeneous solution of the test item.
- Incubation: The closed test flasks were incubated in a climatized chamber under continuous stirring.
- Measuring equipment: The consumption of oxygen was determined by measuring the change of pressure in the flasks. Evolved carbon dioxide was absorbed in an aqueous solution (45%) of potassium hydroxide.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 replicates
- Abiotic sterile control: 1 replicate
- Toxicity control: 1 replicate
- Other: Procedure control: 1 replicate
Test Parameters
- Measurement of Oxygen: The change of pressure in the test flasks was measured by means of a manometric method (BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany) each day.
- Temperature: The temperature was recorded by means of the automated software AMR Wincontrol©.
- pH-Value: pH-values were measured in procedure control, a separately prepared test flask with test item (to prevent loss of test item in the test flasks) and a separately prepared test flask without test item (control) at test start and in all flasks at the end of the test, except in the abiotic and toxicity control, using a pH-electrode WTW pH 340i - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- (ThODNH4)
- Value:
- > 2 - < 3
- Sampling time:
- 28 d
- Details on results:
- The test item Dimethylamine-borane contains nitrogen; therefore, the evaluation of biodegradation has to be based on ThODNH4 and ThODNO3. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the degradation of the test item of at least 60%, reached within a 10-day window; the 10-day window starts when the degradation of the test item reaches at least 10 % degradation. Dimethylamine-borane never reached 10 % biodegradation. After correction for the mean biochemical oxygen demand of the inoculum controls, the mean biodegradation of the test item at test end after 28 days was 3 % (ThODNH4) and 2 % (ThODNO3). See also Figure 1 and 2 further below.
- Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 79 % after 14 days and to 90 % after 28 days of incubation. The percentage biodegradation of the reference item confirms the suitability of the used aerobic activated sludge inoculum.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The mean degradation rate of dimethylamine-borane was 3 % after 28 days. Therefore, dimethylamine-borane is considered to be not readily biodegradable based on ThODNH4 / ThODNO3.
- Executive summary:
The biodegradation of dimethylamine-borane was studied in reconstituted water (pH 7.4 – 7.6) inoculated with activated sludge from a domestic sewage treatment plant (stock suspension of 3.5 g/L on dry matter base and a final sludge concentration in test flasks of 28.7 mg sludge/L). Dimethylamine-borane was applied with 103 mg a.i./L. The experiment was conducted in accordance with the OECD test guideline 301 F ”Ready Biodegradability: Manometric Respirometry Test”, and in compliance with the OECD-GLP standards. The test system consisted of test flasks containing a volume of approximately 500 mL. The pressure decrease in the reaction vessels was measured over complete experimental phase of 28 days using the BSB/BODSensor- System, Aqualytic Dortmund, Germany. The test flasks were closed gas-tight by a measuring head. Potassium hydroxide solution (45%) was used for trapping the produced carbon dioxide. The amount of O2 consumed by the activated sludge was calculated from the decrease of pressure in the reaction vessel.
The test item dimethylamine-borane contains nitrogen; therefore the evaluation of biodegradation has to be based on ThODNH4and ThODNO3. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the degradation of the test item of at least 60 %, reached within a 10-day window; the 10-day window starts when the degradation of the test item reaches at least 10 % degradation. Dimethylamine-borane never reached 10 % biodegradation. After correction for the mean biochemical oxygen demand of the inoculum controls, the mean biodegradation of the test item at test end after 28 days was 3% (ThODNH4) and 2 % (ThODNO3).
The mean degradation rate of dimethylamine-borane was 3 % after 28 days. Therefore, dimethylamine-borane is considered to be not readily biodegradable based on ThODNH4/ ThODNO3.
Reference
Biodegradation in the Toxicity Control
Percentage Biodegradation: In the toxicity control containing both, the test item and the reference item sodium benzoate, 32 % (ThODNH4) biodegradation was noted within 14 days and 36 % (ThODNH4) biodegradation after 28 days of incubation (25 % and 28 % based on ThODNO3). According to the test guidelines the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradation was >25 % within 14 days.
Abiotic Control
Oxygen Demand: The oxygen demand in the abiotic control was 0 mg/L during the test duration. There was no need to correct the degradation of the test item and toxicity control.
Description of key information
The mean degradation rate of dimethylamine-borane in a test according OECD method 301 F was 3 % after 28 days. Therefore, dimethylamine-borane is considered to be not readily biodegradable based on ThODNH4 / ThODNO3.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Hammesfahr (2017) conducted a study on biodegradation in accordance with the OECD test guideline 301 F ”Ready Biodegradability: Manometric Respirometry Test”, and in compliance with the OECD-GLP standards. The test item dimethylamine-borane contains nitrogen; therefore the evaluation of biodegradation has to be based on ThODNH4and ThODNO3. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the degradation of the test item of at least 60 %, reached within a 10-day window; the 10-day window starts when the degradation of the test item reaches at least 10 % degradation. Dimethylamine-borane never reached 10 % biodegradation. After correction for the mean biochemical oxygen demand of the inoculum controls, the mean biodegradation of the test item at test end after 28 days was 3 % (ThODNH4) and 2 % (ThODNO3).
The mean degradation rate of dimethylamine-borane was 3 % after 28 days. Therefore, dimethylamine-borane is considered to be not readily biodegradable based on ThODNH4/ ThODNO3.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)