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EC number: 266-719-9 | CAS number: 67564-91-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18.01. to 29.02.2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: specication: SAG 61.81 Pseudokirchneriella subcapitata
- Source (laboratory, culture collection): At least once per year fresh strains are obtained from the "Sammlung von Algenkulturen" Göttingen (Germany)
- Method of cultivation: according to OECD 201 recommendations - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22 ± 1 °C
- pH:
- 7.93 - 8.04
- Nominal and measured concentrations:
- nominal concentrations: 0.0003, 0.001, 0.003, 0.01. 0.03, 0.1, 0.3 and 1 mg/L;
average measured values were 111% of nominal at test initiation and 90% of nominal at the end of the test, confirming in general the nominal concentrations - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer dimple flasks, 100ml
- Fill volume: 60 ml per replicate
- Initial cells density: 1 x 10^4 cells/mL
- Control end cell number: 1.789 x 10^7 cells/mL
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 10
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Preparation of Test medium: adding 10.35 mg of the test substance to 1000 mL
nutrient solution (to ease solution, the pH was first adjusted to 3.5 - 4);
stock solution was diluted with algal nutrient solution to reach the desired concentrations;
pH of bulk solutions for each concentration was then adjusted again to 8.0
OTHER TEST CONDITIONS
- pH: 8.0 at test initiation
- Photoperiod: continuous light, (white fluorescent lamps);
continuous shaking at 135 rpm.
- Light intensity and quality: about 8000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: in each ask was determined 24, 48, and 72 hours after starting the experiment with a spectrophotometer (623 nm, 5 cm glass cuvettes). Algal medium was used as a blank.
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study: Yes
- Test concentrations: 0, 0.0003, 0.001, 0.003, 0.01, 0.03, 0.1, 0.3 and 1 mg/L - Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.327 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.005 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.058 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Analytical measurements: Analytical verification of the test substance was carried out in each test concentration at the beginning and at the end of the test after 72 hours (with the exception of the two highest concentrations which were determined at the end of the test only). Average measured values were 111% of nominal (range: 94% - 137%) at the beginning and 90% of nominal (range: 73% - 103%) at the end of the test. In addition water samples without algae were analyzed 72 h after test beginning. Recovery results of 85% - 118% underlined the results with the test samples. The following results are therefore based on nominal concentrations.
Biological results: No morphological effects on the algae could be observed up to 0.1 mg/L. At the two highest concentrations, 0.3 mg a.s./L and 1.0 mg a.s./L the algal cells appeared smaller and rounder in comparison to the algal cells in the control.
ErC50 (0-72 h) > 1.0 mg a.s./L
ErC10 (0-72 h) = 0.058 mg a.s./L (95% limits: 0.048 – 0.070 mg a.s./L)
EbC50 (0-72 h) = 0.327 mg a.s./L (95% limits: 0.284 – 0.377 mg a.s./L)
EbC10 (0-72 h) = 0.005 mg a.s./L (95% limits: 0.004 – 0.006 mg a.s./L) - Reported statistics and error estimates:
- Standard procedures, probit analysis
- Conclusions:
- In a 72-hour algae test with Pseudokirchneriella subcapitata the ErC50 of fenpropimorph was determined to be >1.0 mg a.s./L; the ErC10 was 0.058 mg a.s./L (nominal).
Reference
Table 8.2/28 Effect of Fenpropimorph on the growth of Pseudokirchneriella subcapitata | ||||||||
Concentration | 0.0003 | 0.001 | 0.003 | 0.01 | 0.03 | 0.1 | 0.3 | 1.0 |
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Inhibition (biomass) | -10.6 | 7.4 | 6.3 | 14.7 | 21.2 | 38.8 | 47.7 | 63.3 |
Inhibition (growth rate) [%] | -1.9 | 2.3 | 2.4 | 4.6 | 7.5 | 13.5 | 17.2 | 26.1 |
Description of key information
In a 72-hour algae test with Pseudokirchneriella subcapitata the ErC50 of fenpropimorph was determined to be >1.0 mg/L, the ErC10 was 0.058 mg/L (nominal) based on growth rate.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1 mg/L
- EC10 or NOEC for freshwater algae:
- 0.058 mg/L
Additional information
Additionally the effect of the main metabolite fenpropimorph-acid on the growth of the green alga Pseudokirchneriella subcapitata was tested (Dohmen G.P. 1997(b); Reg: 1997/11036) and is summarized below.
No morphological effects on the algae could be observed at any of the test substance concentrations. However the two highest concentrations seemed to have caused morphological changes. Some algal cells appeared smaller and more round in shape.
ErC50 (0-72 h) > 100 mg/L
ErC10 (0-72 h) > 100 mg/L
EbC50 (0-72 h) > 100 mg/L
EbC10 (0-72 h) > 100 mg/L
NOEC = 25 mg/L
In a 72-hour algae test with Pseudokirchneriella subcapitata the ErC50 of fenpropimorph-acid was determined to be >100 mg/L; the ErC10 was >100 mg/L (nominal).
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