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EC number: 266-257-8 | CAS number: 66215-27-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1981/09/29
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Justification for type of information:
- Study was not performed under GLP conditions. The guidelines followed in this study are not stated.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- GLP compliance:
- no
Test material
- Reference substance name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- EC Number:
- 266-257-8
- EC Name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- Cas Number:
- 66215-27-8
- Molecular formula:
- C6H10N6
- IUPAC Name:
- N2-cyclopropyl-1,3,5-triazine-2,4,6-triamine
Constituent 1
- Specific details on test material used for the study:
- The test material is cyromazine.
Sampling and analysis
- Analytical monitoring:
- no
- Remarks:
- No analytical determination of the test item was performed. However, for the determination of the endpoint the amount of carbon dioxide evolved as a result of microbial respiration was measured.
- Details on sampling:
- The amount of carbon dioxide evolved as a result of microbial respiration was measured at 1, 3, 7 14 and 28 days.
Test substrate
- Vehicle:
- yes
- Remarks:
- methanol
- Details on preparation and application of test substrate:
- Soil tested: loam and silt loam soil
Microbial respiration was measured via the amount of carbon dioxide that was evolved.
The soils were blended, screened and sieved before the moisture content of both soils was determined. Available water content of the soils was also determined. Mixing 1000 g of soil with 1000 g of fine sea sand, then adding 1.0% of alfalfa meal prepared test soils. The soil stocks were adjusted to approximately 60% of their field moisture holding capacity. A 400 g fraction of each of the two soil blends was further amended by the addition of 4 mL of methanol (carrier solvent), which was then allowed to evaporate off before the soil blends were again well mixed.
Test organisms
- Test organisms (inoculum):
- soil
Study design
- Total exposure duration:
- 4 wk
Test conditions
- Test temperature:
- 25°C
- Moisture:
- Moisture content of soil measured before test started, refer to 'Details on test conditions'.
- Details on test conditions:
- 2 types of soil used in testing, loam soil and silt loam soil
Loam Soil
Composition: 50.8 % sand, 35.2% silt, 14.0% clay and 2.4% organic matter
pH: 6.7
Moisture Capacity: 33.1%
Silt Loam Soil
Composition: 20.8% sand, 59.2% silt, 20.0% clay and 2.6% organic matter
pH: 6.4
Moisture Capacity: 37.6%
The test treatments and controls were placed in an incubator at 25°C for the 4-week duration of the test. The flasks were aerated twice weekly. - Nominal and measured concentrations:
- nominal concentrations: 100 ppm, 10 ppm and 1 ppm
Results and discussion
Effect concentrations
- Key result
- Duration:
- 4 wk
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- respiration rate
- Details on results:
- In this study, cytomazine at up to 100 mg/kg has no negative effects on microbial soil respiration. In both soils there are samples in which respiration is significantly greater than the control value, however as these incidents follow no pattern their biological significance is doubtful NOEC(respiration) is 100 mg/kg (the highest rate tested).
- Reported statistics and error estimates:
- Data were analyzed by the analysis of variance and mean separation by Duncan's multiple range test.
Any other information on results incl. tables
Table 1: Effects of Cyromazine on soil respiration
Milliequivalents of CO2/ 50g of soil | |||||
Cyromazine | |||||
| Sterile control | Control | 1 mg/kg | 10 mg/kg | 100 mg/kg |
Silt loam soil | |||||
Sampling day |
|
|
|
|
|
1 | 0.13 | 1.17 | 1.22 | 1.16 | 1.27* |
3 | 0.06 | 1.35 | 1.62* | 1.57* | 1.61* |
7 | 0.08 | 1.17 | 1.33* | 1.22 | 1.24 |
14 | 0.05 | 0.98 | 1.06* | 0.96 | 0.95 |
21 | 0.05 | 0.67 | 0.68 | 0.64 | 0.64 |
28 | 0.04 | 0.53 | 0.54 | 0.50 | 0.51 |
Total meq CO2/50g | 0.41 | 5.87 | 6.45 | 6.05 | 6.22 |
Loam soil | |||||
1 | 0.12 | 1.00 | 0.97 | 1.00 | 0.96 |
3 | 0.05 | 1.08 | 1.28* | 1.49* | 1.38* |
7 | 0.06 | 0.99 | 1.53* | 1.46* | 1.56* |
14 | 0.05 | 1.02 | 0.98 | 1.07 | 1.07 |
21 | 0.05 | 1.00 | 1.08 | 1.10* | 1.06 |
28 | 0.04 | 0.67 | 0.87* | 0.87* | 0.87* |
Total meq CO2/50g | 0.37 | 5.76 | 6.71 | 6.99 | 6.90 |
*Significantly different from the control; p< 0.05 (Duncan's multiple range test)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- NOEC (respiration) is 100 mg/kg (the highest rate tested).
- Executive summary:
A four week study investigating the effect of cyromazine on microbial respiration in loam and silt loam soils was carried out under controlled conditions. No guidelines were mentioned or followed in this study. Microbial respiration was measured via the amount of carbon dioxide that was evolved.
Cyromazine was used at concentrations of 100 ppm, 10 ppm and 1 ppm. The test treatments and controls were placed in an incubator at 25°C for the 4-week duration of the test. The amount of Carbon dioxide evolved as a result of microbial respiration was measured at 1, 3, 7, 14 and 28 days.
In this study, cyromazine at up to 100 mg/kg has no negative effects on microbial soil respiration. In both soils there are samples in which respiration is significantly greater than the control value, however as these incidents follow no pattern and their biological significance is doubtful. NOEC (respiration) is 100 mg/kg (the highest rate tested).
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