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EC number: 465-100-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Directive 87/302/EEC, Method C.11.
- GLP compliance:
- yes
Test material
- Test material form:
- solid: particulate/powder
- Details on test material:
- Batch number: 022401
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Details on test solutions:
- A stock solution of nominally 1500 mg/l of "STl571 FB" in deionised water was prepared. Appropriate amounts of these stock solution were added to the different samples at the beginning of the incubation.
A stock solution of 3,5-dichlorophenol (nominally 0.5 /l) was prepared by dissolving 50 mg of the substance in 100 ml of deionised water. Three concentrations, nominally from 5 to 30 mg/l, were prepared.
All incubation mixtures were prepared by mixing 200 ml of microbial inoculum and 200 ml of synthetic sewage solution (see 4.7.2) with 100 ml of tap water or the appropriate dilution of the test or control substance in glass beakers with a nominal volume of 1000 ml.
The samples consisted of two negative controls (one at the beginning and one at the end of the series), five concentrations of "STl571 F8" and three concentrations of the positive control (3,5-dichlorophenol).
After mixing , the samples were aerated at a flow rate of 0.8 Umin using oil free compressed air and a Pasteur-pipette as aeration device.
The incubation of the individual samples was started at inte,rvals of 12-15 min and each sample was aerated for 3 hours at 18 °C. At the end of the incubation time aliquots of the samples wI re transferred to measuring bottles and oxygen consumption was recorded.
nominal concentrations in the incubation mixtures:
"STl571 F8": 7.7, 19.2, 48, 120 and 300 mg/L
3,5-dichlorophenol: 5, 12.2 and 30 mg/L
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The microbial inoculum was a preparation of activated sludge collected on the day of the test. The concentration of the microbial inoculum was adjusted to 4 g of dry weight per litre which gives a final amount of 1.6 g dry weight per litre. The inoculum was kept aerated before the begin of the test.
Study design
- Test type:
- flow-through
- Water media type:
- other: synthetic sewage solution
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- The incubation of the individual samples was started at intervals of 12-15 min and each sample was aerated for 3 hours at 18 °c.
At the end of the incubation time aliquots of the samples were transferred to measuring bottles and oxygen consumption was recorded. - pH:
- The pH of the incubation mixtures after the 3 hour incubation was between 8.27 and 8.32 . The test substance did not influence the pH.
Results and discussion
Effect concentrations
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 162 mg/L
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The test substance inhibited the respiration of the activatec sludge in a concentration dependent extent. The highest obtained inhibition was 63. %.
The dose - response curve of the test substance is presented in Figure 1.
The pH of the incubation mixtures after the 3 hour incubation was between 8.27 and 8.32 . The test substance did not influence the pH.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The test results fulfilled the criteria for validity: • the respiration rates of the two control samples were within 15 % of each other. Actual values:± 0.06 %. • the EC50 of 3,5-dichlorophenol was in the accepted range of 5 to 30 mg/L. Actual EC50: 13.2
- Conclusions:
- The test substance inhibited the respiration of the activated sludge in a concentration dependent extent. The highest obtained inhibition was 63.3 %.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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