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EC number: 700-118-9 | CAS number: 676532-44-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Study period:
- From 10 September 2008 to 28 October 2008
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
- Remarks:
- The exposure concentrations were too high and therefore the results are not reliaable.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 B (Bioaccumulation: Semi-static Fish Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1730 (Fish Bioconcentration Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- other: SEPA. HJ/T 154-2004. The guidelines for the hazard evaluation of new chemical substances. June 2004.
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- other: SEPA. HJ/T 153-2004. The guidelines for the testing of chemicals. June 2004.
- Deviations:
- not specified
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Storage conditions: Refrigerator 4°C
Batch No. : LDP0800001
Purity : 97%
Date of Expiry : 01 May, 2010 - Radiolabelling:
- no
- Details on sampling:
- Sampled water from the test tanks for the determination of test substance concentration before addition of the fish and during the test. Remove seven fish from the test tanks at each sampling time. Rinsed the sampled fish quickly with water, killed instantly, using the most appropriate and humane method, and then weigh.
Sampling days: 0, 3, 7, 10, 14, 21 and 28 days. - Vehicle:
- not specified
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- The test adopted zebra fish (Brachydanio rerio), their average body length was 3.0 +/- 0.5 cm (including the length of their tail). Acclimate the stock population of fish for at least 2 weeks in water at the test temperature and feed throughout on a sufficient diet and of the same type to be used during the test.
- Route of exposure:
- aqueous
- Test type:
- semi-static
- Water / sediment media type:
- natural water
- Total exposure / uptake duration:
- 28 d
- Hardness:
- 140 mg CaCO3/L
- Test temperature:
- no data
- pH:
- 6.5-7.5
- Dissolved oxygen:
- no data
- TOC:
- no data
- Salinity:
- not applicable
- Conductivity:
- no data
- Details on test conditions:
- TEST SYSTEM
- Test vessel: The test tank was glass vessel of 25 mL, made 20 L different concentrations solutions in every vessel, then put in 50 fishes.
- No. of vessels per concentration (replicates): 2
TEST MEDIUM / WATER PARAMETERS
The test water was the natural water which had been settled by placing in the air about 24 hours with total hardness about 140 mg CaCO3/L and pH 6.5-7.5. - Nominal and measured concentrations:
- 5 and 20 mg/L
- Reference substance (positive control):
- no
- Details on estimation of bioconcentration:
- The bioconcentration factor at any time during the uptake phase of this bioconcentration test was the concentration of the test substance (Cf, expressed in milligrams per gram) in/on the fish or specified tissues there of, divided by the concentration of the chemical in the surrounding medium (Cw, expressed in milligrams per litre), as BCF = Cf/Cw.
- Key result
- Conc. / dose:
- 5 mg/L
- Type:
- BCF
- Value:
- 0.37 L/kg
- Basis:
- not specified
- Time of plateau:
- 28 d
- Key result
- Conc. / dose:
- 20 mg/L
- Type:
- BCF
- Value:
- 0.31 L/kg
- Basis:
- not specified
- Time of plateau:
- 28 d
- Details on results:
- See table 5.3.1/1 in "Any other information on results incl. tables".
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The BCF values of the test substance are comprised between 0.313 and 1.07 L/kg.
- Executive summary:
The bioconcentration test was performed using zebra fish (Brachydanio rerio) according to OECD Guideline 305B with GLP statement. The test fishes were exposed to saturated concentration of the test substance 5 and 20 mg/L. BCF values were measured following 28 day exposure period.
The purpose of the study was to determine bioconcentration factors (BCF) for fish exposed to a test chemical in aqueous solution. BCF values for the test substance should always be based on concentrations of the chemical in fish tissue and exposure water, and not on total residues. The concentration factor at any time during the uptake phase of this accumulation test was the concentration of the test substance (expressed in milligrams per gram) in/on the fish or specified tissues there of, divided by the concentration of the chemical in the surrounding medium (BCF = Cf/Cw).
The test substance is detected at retention time approximately 7.2 min with GC-MSD. Linear regression equation: A = 295902 c + 297.3 with good linearity of r² = 0.9996 for concentration 1 -20 mg test substance/L. When add standard storage solution of the test substance to test water with concentration between 1 -20 mg/L, the recovery is between 91.1 -91.8% and RSD was 1.15 -1.40%. When add 5 -100 mg/kg in fish with standard storage solution of the test substance, the recovery is between 91.8 -92.6% and RSD was 1.00 -1.07%. The minimum detection amount of MS detector for the test substance is 1.0 x 10-9g and the minimum detection concentration for water and fish sample are 1 mg/L and 1 mg/kg.
When at the nominal concentration 5 mg/L, the mean BCF value of the test substance in fish is 0.714.
When at the nominal concentration 20 mg/L, the mean BCF value of the test substance in fish is 0.676.
These results show that the BCF value of the test substance is 0.313 -1.07. The bioconcentration factor of the test substance in fish is below 10, so the test substance has low bioconcentration.
Reference
Table 5.3.1/1: Results BCF and concentrations of the test substance in water and fish
Days |
5 mg/L |
20 mg/L |
||||
Water (mg/L) |
Fish (mg/kg) |
BCF |
Water (mg/L) |
Fish (mg/kg) |
BCF |
|
0 3 7 10 14 21 28 |
4.66 4.67 4.58 4.53 4.64 4.57 4.69 |
ND 2.92 4.88 4.49 3.21 2.47 1.72 |
- 0.625 1.066 0.991 0.692 0.540 0.366 |
18.0 18.4 18.8 18.0 18.3 18.7 18.1 |
ND 11.9 18.7 14.6 13.2 10.4 7.90 |
- 0.649 0.996 0.813 0.725 0.557 0.313 |
Mean value |
|
|
0.713 |
|
|
0.676 |
Description of key information
Invalid study
Key value for chemical safety assessment
Additional information
The bioaccumulation experimental study (PEAPC, 2008) was considered as not reliable and was disregarded due to major methodological deficiencies. Indeed, the exposure concentrations were too high and the results are unreliable. The result of this study cannot be considered fit for use.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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