Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 250-784-5 | CAS number: 31736-73-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jul 03 - Sep 14, 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The concentrations of the test item were determined in fresh media (0 hours) and at the end of the exposure interval (72 hours) via GC/MS.
- Vehicle:
- yes
- Remarks:
- water
- Details on test solutions:
- A supersaturated stock solution of 100 mg test item/L was prepared by dissolving 89.2 mg test item into 892 mL test water by intense stirring for 6 hours at room temperature in the dark. Then, non-dissolved fractions of the test item were separated from the test medium by membrane filtration (0.45 μm cellulose acetate filter). Adequate volumes of this stock solution were diluted with test water to prepare the test media of the desired dilutions of 1:20, 1:64, 1:205, 1:655 and 1:2098.
The test media were prepared just before introduction of the algae (= start of the test) - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Pseudokirchneriella subcapitata, Strain No. 61.81 SAG formerly known as Selenastrum capricornutum, and recently renamed as Raphidocelis subcapitata.
Cultivated in the laboratories of ibacon; original source: "Sammlung von Algenkulturen, Albrecht-von-Haller-Institut für Pflanzenwissen-schaften, Universität Göttingen", 37073 Göttingen, Germany. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22.4 to 23.7 °C
- pH:
- pH value in the control at test start: 8.2,
pH value in the control at test end: 10.0;
pH values in the test item treatments at test start: 8.0 to 8.2,
pH values in the test item treatments at test end: 7.8 to 9.9; - Nominal and measured concentrations:
- The dilutions of a filtrate of 100 mg/L were tested: 1:20, 1:64, 1:205, 1:655 and 1:2098 (spacing factor 3.2). Additionally a control was tested in parallel. This corresponds to the following geometric mean measured concentrations of the test item: 6.83, 3.58, 2.28, 1.20 and 0.651 μg test item/L and a control.
- Details on test conditions:
- This study encompassed 6 treatment groups (5 dose rates of the test item and a control) with three replicates per test concentration and six replicates for the control. At test start 50 mL of the test media were inoculated with nominal 5000 algal cells per mL test medium and defined volumes of the algal suspensions were sampled after 24, 48 and 72 hours for determination of cell densities by spectrophotometric measurement.
The dilutions of a filtrate of 100 mg/L were tested: 1:20, 1:64, 1:205, 1:655 and 1:2098 (spacing factor 3.2). Additionally a control was tested in parallel. This corresponds to the following geometric mean measured concentrations of the test item: 6.83, 3.58, 2.28, 1.20 and 0.651 μg test item/L and a control.
Water temperature: 22.4 to 23.7 °C;
pH value in the control at test start: 8.2,
pH value in the control at test end: 10.0;
pH values in the test item treatments at test start: 8.0 to 8.2,
pH values in the test item treatments at test end: 7.8 to 9.9;
continuous illumination; mean light intensity: 5585 lux (5350 to 5870 lux). - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.13 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.42 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.2 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- The 72-hour EyC50 was calculated to be 2.18 μg test item/L and the 72-hour ErC50 value was calculated to be 3.13 μg test item/L. The 72-hour NOEyC was determined to be 1.20 μg test item/L and the associated 72-hour LOEyC was 2.28 μg test item/L. The 72-hour NOErC was determined to be 1.20 μg test item/L and the associated 72-hour LOErC was 2.28 μg test item/L.
The initial concentrations and the maintenance of the exposure concentrations during the test were determined in the analytical part. All reported results refer to geometric mean concentrations, since the test item concentrations were not within ± 20 % of the measured initial concentrations during the test. - Validity criteria fulfilled:
- yes
- Conclusions:
- The purpose of this test was to determine the inhibitory effect of the test item on the growth of the freshwater green algae Pseudokirchneriella subcapitata.
72-hour ErC50: 3.13 μg test item/L
72-hour NOErC: 1.20 μg test item/L
72-hour LOErC: 2.28 μg test item/L.
Reference
Algal Cell Densities during the Test Period of 72 Hours
Geometric mean measured concentration [µg test item / mL] |
Flask No |
Density of algal cells [10000 cells / mL] after |
||
24 h |
48 h |
72 h |
||
Control |
1 |
2.899 |
23.899 |
162.740 |
2 |
2.692 |
26.637 |
156.494 |
|
3 |
2.899 |
26.005 |
148.325 |
|
4 |
3.520 |
27.058 |
161.058 |
|
5 |
2.692 |
25.163 |
155.773 |
|
6 |
3.106 |
27.900 |
165.863 |
|
m |
2.968 |
26.110 |
157.376 |
|
s |
0.312 |
1.427 |
6.223 |
|
0.651 |
1 |
2.485 |
24.741 |
152.890 |
2 |
2.692 |
26.426 |
153.130 |
|
3 |
2.899 |
27.268 |
176.194 |
|
m |
2.692 |
26.145 |
160.738 |
|
s |
0.207 |
1.287 |
13.386 |
|
1.20 |
1 |
2.899 |
20.951 |
137.033 |
2 |
2.485 |
23.268 |
156.013 |
|
3 |
2.485 |
19.056 |
135.592 |
|
m |
2.623 |
21.092 |
142.879 |
|
s |
0.239 |
2.109 |
11.397 |
|
2.28 |
1 |
1.243 |
10.213 |
81.775 |
2 |
1.864 |
9.581 |
74.087 |
|
3 |
1.450 |
8.949 |
71.685 |
|
m |
1.519 |
9.581 |
75.849 |
|
s |
0.316 |
0.632 |
5.271 |
|
3.58 |
1 |
0.621 |
1.790 |
3.694 |
2 |
0.828 |
1.579 |
3.453 |
|
3 |
0.828 |
2.001 |
2.252 |
|
m |
0.759 |
1.790 |
3.133 |
|
s |
0.120 |
0.211 |
0.772 |
|
6.83 |
1 |
0.621 |
0.500 |
0.500 |
2 |
0.621 |
0.500 |
0.500 |
|
3 |
0.621 |
0.500 |
0.500 |
|
m |
0.621 |
0.500 |
0.500 |
|
s |
0.000 |
0.000 |
0.000 |
Description of key information
The 72-hour ErC50 value was calculated to be 3.13 μg test item/L. The 72-hour NOErC was determined to be 1.20 μg test item/L and the associated 72-hour LOErC was 2.28 μg test item/L and the ErC10 was determined to be 1.41 μg test item/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 3.13 µg/L
- EC10 or NOEC for freshwater algae:
- 1.42 µg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.