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EC number: 246-836-1 | CAS number: 25321-14-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Reliability as cited in OECD SIDS Dinitrotoluene (isomers mixture) CAS No.: 25321-14-6, 2004. Comparable to guideline study with acceptable restrictions; only one test concentration shown, no E.coli or TA102 tested
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- The mutagenicity of dinitrotoluenes in Salmonella typhimurium.
- Author:
- Couch B.D. et al.
- Year:
- 1 981
- Bibliographic source:
- Mutat. Res. 90, 373-383
- Reference Type:
- publication
- Title:
- The mutagenicity of dinitrotoluenes in Salmonella Typhimurium.
- Author:
- Couch D.B. et al.
- Year:
- 1 978
- Bibliographic source:
- Environ. Mutagen. 1, 168
- Reference Type:
- secondary source
- Title:
- Dinitrotoluene (isomers mixture), CAS No.: 25321-14-6
- Author:
- OECD SIDS
- Year:
- 2 004
- Bibliographic source:
- OECD SIDS
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- 1000 µg(plate was the maximum concentration; only three concentrations tested; no positive controls used
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dinitrotoluene
- EC Number:
- 246-836-1
- EC Name:
- Dinitrotoluene
- Cas Number:
- 25321-14-6
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- 1-methyl-2,4-dinitrobenzene; 2-methyl-1,3-dinitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): technical grade 2,4-dinitrotoluene (2,4-DNT)
- Isomers composition: 76.5 % 2,4-DNT (purity > 99.89%), 18.8 % 2.6-DNT (purity > 99.89%), 2.4 % 3.4-DNT (purity > 99.89%), 1.5 % 2.3-DNT (purity not confirmed), 0.7 % 2,5-DNT (purity not confirmed), 0.04 % 3,5-DNT (purity > 99.89%)
Constituent 1
Method
- Target gene:
- his-
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA 98, TA 100, TA 1535, TA 1537, TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix from the livers of Aroclor-treated (500 mg/kg, i.p., 5 days prior to sacrifice) male F344 rats
- Test concentrations with justification for top dose:
- 3 concentrations up to 1000 µg/mL
- Vehicle / solvent:
- DMSO was used as solvent.
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- 1% DMSO
- True negative controls:
- no
- Positive controls:
- not specified
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 3 h with and without S9
- Expression time: 40 h incubation at 37 °C
NUMBER OF REPLICATIONS: no data for preincubation test; n=3 for suspension assay
DETERMINATION OF CYTOTOXICITY
- Method: colony forming - Evaluation criteria:
- two-fold increase of number of mutants/plate over control
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium, other: TA 1535, TA 1537, TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: Treatments which caused greater than 90% cytotoxicity relative to solvent controls were not employed.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Species / strain:
- S. typhimurium, other: TA 1538, TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- other: Treatments which caused greater than 90% cytotoxicity relative to solvent controls were not employed.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Results were only shown for the highest concentration tested.
Revertants/plate |
|||||||||||
TA1535 |
TA1537 |
TA1538 |
TA98 |
TA100 |
|||||||
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
||
2,4-DNT technical |
1000 µg/plate |
8 |
13 |
7 |
8 |
98 |
43 |
34 |
56 |
2 |
11 |
DMSO |
1% |
13.3 ± 7.9 |
8.1 ± 3.9 |
4.9 ± 3.0 |
5.1 ± 3.3 |
9.0 ± 6.5 |
15.3 ± 8.8 |
15.5 ± 4.5 |
24.8 ± 8.8 |
74.2 ± 33.5 |
63.1 ± 17.9 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
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