4-(1,4-dioxa-spiro[4.5]dec-8-yl)-cyclohexanone

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Nominal and measured concentrations:

Nominal concentration: 100 mg/L
Means measured concentration: 73 mg/L

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to OECD 202

EFFECT PARAMETERS MEASURED
mortality and mobility after 24 and 48h

RANGE-FINDING STUDY
- Test concentrations: not available
- Results used to determine the conditions for the definitive study: yes, no mortalily up to 100 mg/L

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 73 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

73 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Immobilisation of control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no

The analytical program showed that the actual concentration could be maintained at more than 80 % of the initial concentration. The measured concentration was almost 30% lower than the prepared concentration, probably owing to the method of sample treatment which included an extraction procedure.

Validity criteria fulfilled:

yes

Conclusions:

The test item showed no toxicity towards Daphnia according to OECD 202 up to 73 mg/L (NOEC=73 mg/L, LC50> 73 mg/L mean measured concentration).

Executive summary:

The objective of the study was to determine the acute toxicity of the test item using Daphnia magna according to OECD 202 and EU Method C.2. For this purpose, juvenile Daphnia magna were exposed to an aqueous test material solution over 48 hours under defined conditions. The study comprised of two test vessels per concentration containing ten Daphnia magna each, i.e. 20 Daphnia magna per concentration (test medium group) and control group. Daphnia were exposed to a nominal test material concentration of 100 mg/L (limit test) in an open static system. The measured concentration was almost 30% lower than the prepared concentration, probably owing to the method of sample treatment which included an extraction procedure. Therefore the results are based on the mean measured concentration. Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg/L were not affected. For the test material, the following EC50 values for Daphnia magna were determined: 48h-EC50: > 73 mg/L mean measured concentration, NOEC = 73 mg/L.

Description of key information

The test item showed no toxicity towards Daphnia according to OECD 202 up to 73 mg/L (NOEC=73 mg/L, LC50> 73 mg/L mean measured concentration, reference 6.1.3 -1).

Additional information

The objective of the study was to determine the acute toxicity of the test item using Daphnia magna according to OECD 202 and EU Method C.2. For this purpose, juvenile Daphnia magna were exposed to an aqueous test material solution over 48 hours under defined conditions. The study comprised of two test vessels per concentration containing ten Daphnia magna each, i.e. 20 Daphnia magna per concentration (test medium group) and control group. Daphnia were exposed to a nominal test material concentration of 100 mg/L (limit test) in an open static system. The measured concentration was almost 30% lower than the prepared concentration, probably owing to the method of sample treatment which included an extraction procedure. Therefore the results are based on the mean measured concentration. Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg/L were not affected. For the test material, the following EC50 values for Daphnia magna were determined: 48h-EC50: > 73 mg/L mean measured concentration, NOEC = 73 mg/L (reference 6.1.3 -1).