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Diss Factsheets
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EC number: 419-800-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): A sample of activated sludge was collected from on of the return lines at Burley Menston sewage treatment works (Yorkshire Water), a treatment works whose waste-water catchment is predominantly domestic.
- Storage conditions: On arrival in the laboratory the sample was aerated by means of a compressed air supply delivered through a diffuser block.
- Preparation of inoculum for exposure: The suspended solids concentration was determined by filtering a 25 mL subsample through a pre-weighed glass microfibre filter (Whatman GF/C). The filter and retained solids were then dried at a nominal 105°C, re-weighed and the contribution made by the sludge solids determined by difference.
- Pretreatment: Not pretreated with the test substance.
- Concentration of sludge: 30 mg/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 15 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The test was conducted in synthetic mineral salts medium based on distilled water as per the OECD guideline.
- Additional substrate: None
- Solubilising agent (type and concentration if used): None
- Test temperature: In the range 20 to 22°C.
- pH:
Control Day 0, 7.69 to 7.72 and Day 28, 7.58 to 7.63.
Test substance Day 28, 7.77 to 7.83
Reference substance Day 0, 7.70 to 7.71, and Day 28 7.95 to 7.97.
Toxicity control: Day 28, 8.24.
The pH in the test substance and toxicity control vessels were not assessed on Day 0 as the addition of pH probes into the medium may have removed test substance from the vessels.
- pH adjusted: No
- Aeration of dilution water: Yes.
- Suspended solids concentration: The final suspended solids concentration in all vessels was nominally 10 mg/L.
- Continuous darkness: Yes
TEST SYSTEM
- Culturing apparatus: Not specified but assumed to be standard BOD bottles.
- Number of culture flasks/concentration: Two replicates for the test substance, control and reference control. One for the toxicity control.
- Method used to create aerobic conditions: Vessels purged with air
- Test performed in open system: No
- Details of trap for CO2 and volatile organics if used: Nominally CO2 free control air fed from cylinders. As an added precaution, the flow passed through a column packed with Carbosorb AS, a self indicating, artificial silicate CO2 absorber.
- Dosing the test substance: The test substance was weighed onto PTFE discs and added together with the weighing supports to the two test vessels and toxicity control.
SAMPLING
- Sampling frequency: Days 2, 4, 6, 8, 10, 14, 19, 24 and 28.
- Sampling method: Scrubbers were detached at appropriate intervals and their contents titrated with acid to determine the quantity of CO2 purged from the respective test vessels.
- Sterility check if applicable: Not applicable
- Sample storage before analysis: Determined soon after scrubbers detached.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes (2 replicates)
- Abiotic sterile control: No
- Toxicity control: Yes (1 replicate)
STATISTICAL METHODS: Not required. - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- Not a guideline requirement
- Test performance:
- Validity criteria:
- The reference compound satisfied the requirements for classification as readily biodegradable by Day 14. The percentage degradation exceeded 60% of theoretical within ten days of first reaching 10% and the individual percentage degradation data recorded in the duplicate reference vessels lay within 20% of one another throughout the study.
- CO2 blank generation: Total mean blank CO2 production in this study was 38.83 mg/3L, indicating that respiration of the inoculum had not been of sufficient magnitude to cause interference with test results. The guideline states blanks should not exceed 120 mg/3L of medium. - Parameter:
- % degradation (CO2 evolution)
- Value:
- 1
- Sampling time:
- 28 d
- Details on results:
- Degradation at the end of the study failed to reach the 60% 'pass' level and accordingly the substance was not considered to be readily biodegradable
- Results with reference substance:
- Measured CO2 production exceeded the 'pass' level at 60% of theoretical within 14 days and within the ten day window.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- In accordance with the OECD 301B guideline, no significant production of CO2 was registered at the applied concentration of the test substance over the 28 day test period. Therefore, the substance is not considered to be readily biodegradable.
Reference
Biodegradation as percentage of theoretical CO2 yield:
Days |
Test substance |
Reference substance |
|||
Rep. 1 |
Rep. 2 |
Tox control |
Rep. 1 |
Rep. 2 |
|
2 |
0 |
0 |
14 |
21 |
21 |
4 |
0 |
0 |
39 |
45 |
44 |
6 |
0 |
0 |
57 |
60 |
59 |
8 |
1 |
1 |
69 |
69 |
68 |
10 |
1 |
1 |
74 |
73 |
73 |
14 |
1 |
1 |
79 |
77 |
77 |
19 |
1 |
1 |
83 |
81 |
82 |
24 |
1 |
1 |
86 |
85 |
86 |
28 |
1 |
1 |
91 |
90 |
90 |
Description of key information
In an OECD guideline, GLP study only minimal degradation was observed. Accordingly the substance would not be considered readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.