3-acetamido-5-amino-4-hydroxybenzenesulphonic acid

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13.-22.01.1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Remarks:

Deion. water instead of dest. water for the stocksolution; Preparation of the inoculum:higher sludge weight after the washing procedure (Sludge concentrations in the test according to the guidelines); 4 instead of 5 test concentrations

Principles of method if other than guideline:

Deviation from the guideline
- Deion. water instead of dest. water for the stocksolution.
- Preparation of the inoculum :higher sludge weight after the washing procedure (Sludge concentrations in the test according to the guidelines)
- 4 instead of 5 test concentrations

GLP compliance:

yes

Specific details on test material used for the study:

Code Number: FAT - 92'333/A
Batch number: K-37. 19.5.92
Purity: 97.2%
Appearance:solid:
Solubility:1.3 g/l in water
Storage: room temperature
Expiration date:June 1993
Safety: Test substance will, unless otherwise specified, be handled using safety precautions "Sicherheit im Labor von A - Z", SAV: 00 303; Test substance will, unless otherwise specified, be handled using safety precautions "Sicherheit im Labor von A - Z", SAV: 00 303

Reference substance:
3,5-Dichlorophenol (Merck 821768)

Test organisms (species):

sewage, domestic

Details on inoculum:

Test system / Test organism
Organism: Activated sludge of a communal sewage treatment plant (ARA-Pro Rheno Basel)
Sludge pretreatment: 3 times washing with tap water and addition of synthetic sewage (a day before the main test)

Test type:

static

Water media type:

freshwater

Total exposure duration:

3 h

Test temperature:

22.0 °C

pH:

6.5

Dissolved oxygen:

0,1 - 0.2 l/min

Details on test conditions:

Experimental conditions
Temperature: 22.0 °C
Sludge dry weight: 1.50 g/l
Test volume: 100 ml
Test duration: 3h
Estimation technique: O2 - consumption, measured with an oxygen sensitive electrode system: OXI DIGI 530 (SAV : 00 109)
Aeration: 0,1 - 0.2 l/min by pasteur-pipett
pH of sludge: 6.5 before the test beginning


Test concentrations:
320,100, 32 and 10 mg/l of the test substance
32, 10 and 3.2 mg/l of the reference substance

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 320 mg/L

Details on results:

IC50 of FAT - 92'333/A : > 320 mg/l

Results with reference substance (positive control):

IC50 of the reference substance : 7.8 mg/l

IC50:

The respiration rate of each test concentration was calculated as a percentage of the mean of the two control respiration rates. The results were plotted on a logarithmic (concentration)/normal (% inhibition) graph, where the IC5Q can be evaluated.

Validity criteria fulfilled:

yes

Conclusions:

not classified

Executive summary:

The toxicity of FAT - 92'333/A to activated sludge was determined in a 3-hours respiration inhibition test according to the OECD Guideline No. 209. and to the EG Method Nr.L 133/118. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance concentrations tested were 320.100. 32 and 10 mg/l. The reference substance 3.5-Dichlorophenol was tested in concentrations of 32,10 and 3.2 mg/l.

Results: IC50 of FAT - 92'333/A : > 320 mg/l

IC50 of the reference substance : 7.8 mg/l

Description of key information

The toxicity of FAT - 92'333/A to activated sludge was determined in a 3-hours respiration inhibition test according to the OECD Guideline No. 209. and to the EG Method Nr.L 133/118. The IC50 of FAT - 92333/A was >320 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

320 mg/L