Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 266-235-8 | CAS number: 66204-44-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water and sediment: simulation tests
Administrative data
- Endpoint:
- biodegradation in water: simulation testing on ultimate degradation in surface water
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Study according to guideline (OECD 306) in compliance with GLP (without certificate). The pollutional and nutritional status of the seawater is not described in the study report. 2 replicates for both test item and reference substance and 5 replicates for the blank control were used, instead of each 8 proposed by the Guideline. No toxicity control was run. O2 consumption in the control vessels was slightly higher (mean 35%) than decribed in the Guideline (30%). However, these deviations are not expected to have a significant influence on the conclusion. Therefore, the test is considered to be valid. Seawater sample was not fully described (depth of collection, DOC), description of stock solution is incomplete, possible interference from oxygen uptake by nitrification was not addressed.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 306 (Biodegradability in Seawater)
- Version / remarks:
- (Closed Bottle Procedure)
- Deviations:
- yes
- Remarks:
- Pollutional and nutritional status of seawater not reported. 2 replicates for test item and reference and 5 replicates for blank were used instead of 8 each. No toxicity control was run. O2 cons. in control slightly higher than decribed in Guideline.
- GLP compliance:
- yes
- Remarks:
- No certificate but a Quality Assurance Statement is available
Test material
- Reference substance name:
- 3,3'-methylenebis[5-methyloxazolidine]
- EC Number:
- 266-235-8
- EC Name:
- 3,3'-methylenebis[5-methyloxazolidine]
- Cas Number:
- 66204-44-2
- Molecular formula:
- C9H18N2O2
- IUPAC Name:
- 3,3'-methylenebis[5-methyloxazolidine]
- Details on test material:
- - Reaction products of paraformaldehyde and 2-hydroxypropylamine (ratio of 3:2)
- Specification: GrotaMar 71®
Constituent 1
- Specific details on test material used for the study:
- The original study refers to MAR 71 as test substance.
- Radiolabelling:
- no
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Natural seawater
- Details on inoculum:
- - Sampling site: MAFF site at Conway, North Wales (UK)
- Laboratory culture: No
- Pretreatment: Seawater (pH 8.12) was coarse filtered, maintained in the dark. Aged prior to use by gentle aeration for three weeks at 20 ± 2°C. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 2.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST SYSTEM
- Culturing apparatus: BOD bottles, 272 mL, completely filled
- Number of culture flasks/concentration: 2 (test item and reference substance) 5 (control)
TEST CONDITIONS
- Composition of medium: According to Acer Environmental SOP III.36
- Additional substrate: The seawater was fortified with nutrients (no data about nature and amounts)
- Test temperature: 20 ± 1°C
- pH: 8.12 at collection
- Salinity: 29.9 per mil
- Aeration of dilution water: No
SAMPLING
- Oxygen measured at days 0, 5, 10, 15, 22, and 28
CONTROL AND BLANK SYSTEM
- 5 control vessels without test substance
STATISTICS
- Mean values of replicated were used for calculations
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- Initial concentration 2 mg/L
Results and discussion
% Degradationopen allclose all
- % Degr.:
- 69.4
- Parameter:
- DOC removal
- Sampling time:
- 28 d
- Remarks on result:
- other: calculated as BOD/COD (Initial concentration 2.5 mg/L)
- % Degr.:
- 71.4
- Parameter:
- DOC removal
- Sampling time:
- 22 d
- Remarks on result:
- other: calculated as BOD/COD (Initial concentration 2.5 mg/L)
- % Degr.:
- 62.6
- Parameter:
- DOC removal
- Sampling time:
- 15 d
- Remarks on result:
- other: calculated as BOD/COD (Initial concentration 2.5 mg/L)
- % Degr.:
- 27.4
- Parameter:
- DOC removal
- Sampling time:
- 10 d
- Remarks on result:
- other: calculated as BOD/COD (Initial concentration 2.5 mg/L)
- % Degr.:
- 11.3
- Parameter:
- DOC removal
- Sampling time:
- 5 d
- Remarks on result:
- other: calculated as BOD/COD (Initial concentration 2.5 mg/L)
- % Degr.:
- 53.8
- Parameter:
- DOC removal
- Sampling time:
- 28 d
- Remarks on result:
- other: calculated as BOD/COD (Initial concentration 5.0 mg/L)
- % Degr.:
- 62.8
- Parameter:
- DOC removal
- Sampling time:
- 22 d
- Remarks on result:
- other: calculated as BOD/COD (Initial concentration 5.0 mg/L)
- % Degr.:
- 56.8
- Parameter:
- DOC removal
- Sampling time:
- 15 d
- Remarks on result:
- other: calculated as BOD/COD (Initial concentration 5.0 mg/L)
- % Degr.:
- 8.3
- Parameter:
- DOC removal
- Sampling time:
- 10 d
- Remarks on result:
- other: calculated as BOD/COD (Initial concentration 5.0 mg/L)
- % Degr.:
- 8.3
- Parameter:
- DOC removal
- Sampling time:
- 5 d
- Remarks on result:
- other: calculated as BOD/COD (Initial concentration 5.0 mg/L)
- Results with reference substance:
- 77.5 % BOD/ThOD after 28 days
Any other information on results incl. tables
If BOD is related to ThOD NO3 (2.577 mg O2/mg) than after 28 days degradation is 48.7% (2.5 mg/L) and 37.7% (5 mg/L).
Applicant's summary and conclusion
Validity criteria
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance reached the pass-levels for biodegradation. The substance has a potential for biodegradation in seawater.
- Executive summary:
The results indicate that the substance has a potential for biodegradation in seawater at both concentrations. After 28 days of incubation, the degradation (calculated as BOD/COD) was 69.4% at 2.5 mg/L and 53.8% at 5.0 mg/L. However, the higher concentration (5 mg/L) had also achieved 62.8% degradation by day 22 at which point almost all the oxygen had been consumed. The pass level was reached within 14 d at both concentrations. All other validity criteria of the OECD Guideline were met.
During the study, the COD was measured to be 1.8089 mg O2/mg. This value is similar to the ThODNH3 of 1.89 mg O2/mg when nitrification is not considered. For total oxidation a ThODNO3of 2.58 mg O2/mg is calculated. The degradation percentages calculated on the latter value, where nitrification is considered, would be lower. The pollutional and nutritional status of the seawater is not described in the study report. However, the O2consumption in the control vessels was slightly higher (mean 35%) than described in the Guideline (30%) which might be caused by nutrients present in the seawater. This small deviation is considered to have no significant influence on the test results. In the study 2 replicates for both test item and reference substance and 5 replicates for the blank control were used, instead of each 8 proposed by the Guideline. This deviation is considered to have no significant influence on the test results. No toxicity control was run. However, results from tests on inhibition to microbial activity indicate that the test was conducted in a non-toxic concentration range, the EC50was determined to be 44 mg/L in both tests.
Studies on hydrolysis of the substance indicate that at large dilutions the hydrolysis products are expected which are both readily biodegradable. Therefore, the substance and the hydrolysis products are expected to be extensively removed in biological treatment plants as well as in environmental compartments.
The constituents of the reaction product (substance) are not volatile from aqueous solutions, therefore this removal mechanism could not influence the test results. It is not expected that the low adsorptivity of the test substance influenced the oxygen uptake.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.