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EC number: 425-560-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 SEP 2010 - 8 NOV 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
- Version / remarks:
- before 2 Oct 2012
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: “Bioaccumulation test of chemical substance in fish " stipulated in “the Order Prescribing the Items of the Test Relating to the New Chemical Substance”
- Deviations:
- no
- GLP compliance:
- yes
- Radiolabelling:
- not specified
- Details on sampling:
- - Sampling intervals/frequency for test organisms: day 7, 14, 21, 26, 28
- Sampling intervals/frequency for test medium samples: day 7, 14, 21, 26, 28
Test water:
・Sampling: 200ml of Concentration I and II
・Placed them still for a while and let the floating stuffs settle.
・Measurement: 100ml of Concentration I and II (by graduated cylinder)
・Moved into the 200mL-separating funnel
← Washed inside of the cylinder with a small amount of Ultra-purewater
← Dichloromethane
・Shaker (dial7, 5 mis)
・Placed (until the 2 layers become transparent)
- Upper layer (water) - Bottom layer (Dichloromethane)
- Bottom layer (Dichloromethane) - Upper layer (water)
Filtrate
・Evaporator (water bath 35℃)
・Dried by air spray
・Ultra-pure water / THF/Acetonitrile
・Quantification
Concentration I: 25ml
Concentration II: 5ml
Quantified solution
HPLC
Analyzing test water of control group (at the beginning of the test) was in accordance with the method for Concentration II.
Fish body
Test fish (2 fish paired)
・Measurement: full length, body weight
・Chopping
・Mover into the homogenizer cup
・Homogenize (9000 r.p.m, 6 mins)
・Washing
・Put the washing fluid into the homogenizer cup
・Moved the content into the centrifuge tube
・Washing
・Moved the washing fluid into the centrifuge tube
・Adjusted the weight of centrifuge tube with a small amount of Acetonitrile
・Centrifugation (9000 r.p.m, about 10℃, 15 mins)
Supernatant Residue
・Filtration (Glass filter GC50 / Advantech Toyo
・Filtrated the washing fluid and put into the previous measuring flask
・Waited until the temperature of the solution becomes room temperature
・Quantification 100ml (Acetonitrile)
Quantified solution
・2.5ml taken by whole pipette
・Moved into the100mL-Egg-plant flask
・Evaporator (water bath 40℃)
・Dried by air spray
Sep-Pak Florisil
・Conditioning
・Elution
・Taken in the 50mL-Egg-plant flask
・Evaporator (water bath 40℃)
・Dried by air spray
・Quantification 1ml
Quantified solution
HPLC
The flow rate into the Sep-Pak Florisil was set about 3ml/min. - Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- 0.15 mg/L(1st concentration) and 0.015 mg/L(2nd concentration) of Tween 80 was used as solubilising agent
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name:carp - Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
- Hardness:
- not specified
- Test temperature:
- not specified
- pH:
- not specified
- Dissolved oxygen:
- not specified
- TOC:
- not specified
- Salinity:
- not specified
- Conductivity:
- not specified
- Details on test conditions:
- TEST SYSTEM
- No. of organisms per concentration (replicates): 50
- No. of organisms per control / vehicle control (replicates): 15
PRELIMINARY STUDY (Acute toxicity test)
- Test concentrations: 31 mg/L (limit test)
- Results used to determine the conditions for the definitive study: LC50 (96hr) > 31 mg/L(Limit test), Danio rerio, solubilizing agent: Tween 80, 100 mg/L - Nominal and measured concentrations:
- 0.05 mg/L (1st concentration), 0.005 mg/L (2nd concentration)
0.0457 - 0.0520 mg/L, 0.00468 - 0.00502 mg/L - Reference substance (positive control):
- not specified
- Details on estimation of bioconcentration:
- BASIS INFORMATION
- Measured/calculated logPow: >4.7 - Lipid content:
- 4.7 %
- Time point:
- start of exposure
- Lipid content:
- 4.6 %
- Time point:
- end of exposure
- Conc. / dose:
- 0.15 mg/L
- Type:
- BCF
- Value:
- >= 3 - <= 20 dimensionless
- Basis:
- whole body w.w.
- Time of plateau:
- 28 d
- Calculation basis:
- steady state
- Conc. / dose:
- 0.005 mg/L
- Type:
- BCF
- Value:
- >= 25 - <= 31 dimensionless
- Basis:
- whole body w.w.
- Time of plateau:
- 28 d
- Calculation basis:
- steady state
- Details on results:
- Recovery rate (average)
Recovery from water (%): Concentration I: 94.5%
Concentration II: 94.2%
Recovery from fish (%): 86.2% - Validity criteria fulfilled:
- not specified
- Conclusions:
- During 28 days exposure period BCF values showed less than 50 fold through 28 days’ exposure period. Therefore 28 days after the start of exposure the concentration rate was regarded to reach steady state and the test was finished.
Bioconcentration factors (BCF) of the test substance in carp were 3 to 20 fold for the 1st concentration and 25 to 31 fold for the 2nd concentration. Hence, the substance is not regarded as bioaccumulative. - Executive summary:
The study was conducted in compliance with GLP regulations and in accordance with the OECD Test Guideline No.305 "Bioconcentration : Flow-through Fish test” (before 2nd October 2012).
10 fishes of Danio rerio (Zebra fish) were used for acute toxicity test and 100 mg/L of Tween 80 was used as solubilising agent. LC50(96hr) was defined as >31 mg/L (limit test).
115 fishes (50 fishes for each test concentration, 15 fishes for control) of Cyprinus carpio were used for the bioaccumulation test. Based on 96 hours LC50 value the tests were performed in each 0.05 mg/L (1st concentration), 0.005mg/L (2nd concentration) of the test substance concentration and 0.15 mg/L (1st concentration) 0.015 mg/L (2nd concentration) of Tween 80 was used as solubiliging agent. Analysis of fish was done on 7th, 14th, 21st, 26th and 28th days after the start of exposure.
Result
Concentration test result
Uptake period
7 day
14 day
21 day
26 day
28 day
1st concentration
Test substance concentration in water (mg/L)
0.0457
0.0495
0.0520
0.0506
0.0476
Concentration
rate
8
9
8
20
6
10
8
12
7
3
2nd concentration
Test substance concentration in water (mg/L)
0.00477
0.00502
0.00494
0.00494
0.00468
Concentration
rate
<11
<11
31
27
<12
<11
25
<12
25
<12
Average recovery rate from water was determined as 94.5 % (1st concentration) and 94.2 % (2nd concentration) and average recovery rate from fish was determined as 86.2 %.
Theoretical detection limit of BCF value was determined as ≧ 1 (1st concentration) and ≧ 12 (2nd concentration).
During 28 days exposure period BCF values showed less than 50 fold through 28 days’ exposure period. Therefore 28 days after the start of exposure the concentration rate was regarded to reach steady state and the test was finished.
Bioconcentration factors (BCF) of the test substance in carp were 3 to 20 fold for the 1st concentration and 25 to 31 fold for the 2nd concentration. Hence, the substance is not regarded as bioaccumulative.
Reference
Concentration test results
|
Uptake period |
Day 7 |
Day 14 |
Day 21 |
Day 26 | Day 28 |
Concentration I |
Test substance concentration in water (mg/L) |
0.0457 | 0.0495 | 0.0520 | 0.0506 | 0.0476 |
concentration rate | 8 10 |
9 8 |
8 12 |
20 7 |
6 3 |
|
Concentration II | Test substance concentration in water (mg/L) |
0.00477 |
0.00502 |
0.00494 |
0.00494 |
0.00468 |
|
Concentration rate |
<11 <11 |
<11 25 |
31 <12 |
27 25 |
<12 <12 |
Description of key information
Key, Bioconcentration, Cyprinus carpio, 28 d, OECD 305, GLP: BCF = 31, not bioaccumulative
Key value for chemical safety assessment
- BCF (aquatic species):
- 31 dimensionless
Additional information
OECD 305
The study was conducted in compliance with GLP regulations and in accordance with the OECD Test Guideline No.305 "Bioconcentration : Flow-through Fish test” (before 2nd October 2012).
10 fishes of Danio rerio (Zebra fish) were used for acute toxicity test and 100 mg/L of Tween 80 was used as solubilising agent. LC50(96hr) was defined as >31 mg/L (limit test).
115 fishes (50 fishes for each test concentration, 15 fishes for control) of Cyprinus carpio were used for the bioaccumulation test. Based on 96 hours LC 50 value the tests were performed in each 0.05 mg/L (1st concentration), 0.005mg/L (2nd concentration) of the test substance concentration and 0.15 mg/L (1st concentration) 0.015 mg/L (2nd concentration) of Tween 80 was used as solubiliging agent. Analysis of fish was done on 7th, 14th, 21st, 26th and 28th days after the start of exposure.
Result
Concentration test result
|
Uptake period |
7 day |
14 day |
21 day |
26day |
28 day |
1st concentration |
Test substance concentration in water (mg/L) |
0.0457 |
0.0495 |
0.0520 |
0.0506 |
0.0476 |
Concentration rate |
8 |
9 |
8 |
20 |
6 |
|
10 |
8 |
12 |
7 |
3 |
||
2nd concentration |
Test substance concentration in water (mg/L) |
0.00477 |
0.00502 |
0.00494 |
0.00494 |
0.00468 |
Concentration rate |
<11 |
<11 |
31 |
27 |
<12 |
|
<11 |
25 |
<12 |
25 |
<12 |
Average recovery rate from water was determined as 94.5% (1st concentration) and 94.2% (2nd concentration) and average recovery rate from fish was determined as 86.2%.
Theoretical detection limit of BCF value was determined as ≧1 (1st concentration) and ≧12 (2nd concentration).
During 28 days exposure period BCF values showed less than 50 fold through 28 days’ exposure period. Therefore 28 days after the start of exposure the concentration rate was regarded to reach steady state and the test was finished.
Bioconcentration factors (BCF) of the test substance in carp were 3 to 20 fold for the1st concentration and 25 to 31 fold for the 2nd concentration.
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