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EC number: 250-280-5 | CAS number: 30673-36-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 - 21 Sep 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Date of certificate: 14.03.2017 (The Department of Health of the Government of the United Kingdom)
Test material
- Reference substance name:
- Butyl decanoate
- EC Number:
- 250-280-5
- EC Name:
- Butyl decanoate
- Cas Number:
- 30673-36-0
- Molecular formula:
- C14H28O2
- IUPAC Name:
- butyl decanoate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations, taken at start and end of the 48 hour exposure period.
- Sampling method: Samples were taken from remaining test media after filling test vessels for 0 hours and pooled replicate flasks for 48 hours.
Test solutions
- Vehicle:
- yes
- Remarks:
- Dichloromethane
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solutions were prepared separately by adding the appropriate amount of test substance to solvent (DCM). The total volume of the solvent in the stock solution was 10 mL. The test concentrations were prepared by adding 5 µL of the corresponding stock solution to a total volume of 50 mL of media water.
- Chemical name of vehicle : Methanol for the range finding test and Dichloromethane (DCM) for the definitive test.
- Volume of solvent in solvent control: 5 µL
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: From cultures maintained at Chemex Environmental International Ltd since July 2014 and originally sourced from a clone of genotype 5
- Age of parental stock: ≤ 24 hours
- Feeding during test: no
CULTURE CONDITIONS
- Cultured under static conditions
- Temperature: 20 ± 2°C
- Dissolved oxygen: >60% (or 3 mg/L) ASV
- Photoperiod: 16 hours light and 8 hours dark
- Acclimation conditions: same as in test
- Type of food: suspension of Chlorella vulgaris.
- Feeding frequency: Each working day
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20.7 - 21.9 °C
- pH:
- 7.30 - 7.63
- Dissolved oxygen:
- 6.04 - 7.16 mg/L
- Nominal and measured concentrations:
- Nominal test concentrations:control, solvent control, 0.134, 0.429, 1.370, 4.380, 14.0 and 45 mg/L
Mean measured concentrations: control, solvent control, 0.059, 0.073, 0.064, 0.085, 0.169 and 0.179 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Plastic pots (nominal volume 50 mL), covered with a perspex sheet
- Fill volume: 50 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 reconstituted Daphnia water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 hours dark cycle
EFFECT PARAMETERS MEASURED : The number of immobilised Daphnia was recorded after 24 and 48-hour exposure.
VEHICLE CONTROL PERFORMED: yes
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: solvent control (methanol), 0 (control), 0.1, 1.0, 10, 50 mg/L
- Results used to determine the conditions for the definitive study: EC50 (72 h): 10 - 50 mg/L (nominal) - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (3.5 - 6.4 mg/L; 95% confidence limits)
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.09 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (0.08 - 0.14 mg/L; 95% confidence limits)
- Details on results:
- - Mortality of control or solvent control: No
- Other adverse effects control or solvent control: No
- Results with reference substance (positive control):
- EC50 (24 h): 1.4 mg/L of potassium dichromate (obtained in a separate GLP study conducted from 18 to 20 January 2017)
- Reported statistics and error estimates:
- ToxCalc™ Version 5.0 “Comprehensive Toxicity Data Analysis and Database Software”, copyright 1994-1996.
Any other information on results incl. tables
Table 1: Cumulative immobilisation
Nominal Concentration mg/L |
Mean measured concentrations mg/L |
Number immobilised |
% immobilisation |
||
24 hours |
48 hours |
24 hours |
48 hours |
||
0 (Control) |
0 (Control) |
0 |
0 |
0 |
0 |
Solvent control (DCM) |
Solvent control (DCM) |
0 |
0 |
0 |
0 |
0.134 |
0.059 |
0 |
0 |
0 |
0 |
0.429 |
0.073 |
0 |
0 |
0 |
0 |
1.370 |
0.064 |
0 |
0 |
0 |
0 |
4.380 |
0.085 |
6 |
10 |
30 |
50 |
14 |
0.179 |
10 |
19 |
50 |
95 |
45 |
0.169 |
14 |
20 |
70 |
100 |
Table 2 Biological observations
Nominal Concentration mg/L |
Recorded observations |
|
24 hours |
48 hours |
|
0 (Control) |
20NS |
20NS |
Solvent control (DCM) |
20NS |
20NS |
0.134 |
13NS5Ms2TM |
16NS2Ms2TM |
0.429 |
16NS1Ms3TM |
9NS9Ms1TMLI1TM |
1.370 |
4NS2Ms14TM |
4NS7Ms9TM |
4.380 |
4TM4TMD2DMs4TMDMs |
2TM3TMMs1Ms2TMDMs1MsLI1TMLIMs |
14 |
10TMDMs |
1MsD |
45 |
6TMDMs |
- |
Key to comments |
|||
S |
Sedimentation |
Ms |
Movement Slower |
D |
Debris attached to Daphnia |
TM |
Trapped in Meniscus (mobile) |
LI |
Lighter Colouration |
TI |
Trapped in Meniscus (immobile) |
NS |
No Signs of Abnormality |
SM |
Daphnia Smaller |
- |
All Daphnia immobilised |
Table 3: Validity criteria for OECD 202.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0% |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
>6 mg/L |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- see Table 3 at "Any other information on results incl. tables"
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