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EC number: 834-791-6 | CAS number: 1310681-27-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- other information
- Study period:
- 2018-06-04 to 2018-07-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2011
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- [2,2,6,6-tetramethyl-4-(trimethylazaniumyl)piperidin-1-yl]oxidanyl chloride
- EC Number:
- 814-375-0
- Cas Number:
- 67036-09-3
- Molecular formula:
- C12H26N2OCl
- IUPAC Name:
- [2,2,6,6-tetramethyl-4-(trimethylazaniumyl)piperidin-1-yl]oxidanyl chloride
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: nominal test substance concentrations of 100, 31.3, 9.77, 3.05, 0.954 mg/L and control
- Sampling method: 1 mL analytical samples were taken at 0 h and 72 h.
- Sample storage conditions before analysis: stored deep frozen
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 1000 mg/L was prepared by weighing the test substance on a weighing scoop and transfering to a flask. Test medium was added and the solution homogenised by shaking. Lower test concentrations were prepared by dillution of the stock solution.
- Test medium: Test concentrations were prepared with AAP-medium.
- Controls: Test medium without test substance, 6 replicates
- Evidence of undissolved material: The stock solution was clear and yellowish.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: P. subcapitata Hindak, SAG-61.81
- Source: commercial supplier
- Age of inoculum (at test initiation):
- Method of cultivation: Cultivation in sterile cultures in the laboratory with periodical replacement of old medium. Stock cultures are ordered regularly from commercial supplier.
ACCLIMATION
- Acclimation period: 3 to 4 days before start of the test, test medium was inoculated with the test organism for a pre-culture.
- Culturing media and conditions: same
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.5 – 23.3 °C
- pH:
- The pH-value of the control ranged from 7.54 to 8.38 during the test period
- Nominal and measured concentrations:
- nominal test substance concentrations of 100, 31.3, 9.77, 3.05, 0.954 mg/L (spacing factor: 3.2) and control. The measured initial concentration was in the range of 94-95%, the concentration in aged medium was in the range of 99% to 101% of the nominal concentration. Therefore, the effect values are based on the nominal concentration.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL Erlenmeyer flasks
- Type: closed with aluminium caps
- Material, size, headspace, fill volume: 100 mL flask volume, 50 mL fil/test volume
- Aeration: continuous agitation
- Renewal rate of test solution: static test
- Initial cells density: 0.5*10^4 cells/mL
- Control end cells density: 4.354 *10^5 cells/mL
- No. of organisms per vessel: 2.5*10^5 cells/test vessel
- No. of vessels per concentration (replicates): 3 replicates per concentration
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes, AAP -medium, according to guideline, Annex 3
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no. The pH of the test medium was adjusted to 7.5 before the test.
- Photoperiod: Continuously
- Light intensity and quality: 88.1 µEm-2s-1 (mean)
EFFECT PARAMETERS MEASURED :
- Determination of cell concentrations: Fluorescence measurements in a microplate reader at 670 nm, in duplicate, at 0 h, 24, 48, and 72 h
- Other: Morphological appearance was examined microscopically at the test end.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
Range finding study
- Test concentrations: 100, 10, 1.0, and 0.1 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 31.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Substance specific analytic show good recovery. Therefore, the results were based on nominal concentrations.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Substance specific analytic show good recovery. Therefore, the results were based on nominal concentrations.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Substance specific analytic show good recovery. Therefore, the results were based on nominal concentrations.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Substance specific analytic show good recovery. Therefore, the results were based on nominal concentrations.
- Basis for effect:
- cell number
- Remarks:
- yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 31.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Substance specific analytic show good recovery. Therefore, the results were based on nominal concentrations.
- Basis for effect:
- cell number
- Remarks:
- yield
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Substance specific analytic show good recovery. Therefore, the results were based on nominal concentrations.
- Basis for effect:
- cell number
- Remarks:
- yield
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): The cells were considered normal for the control and for the test substance concentrations.
The growth conditions (pH and temperature) during the test were within the range specified in the guideline.
After 72 h at termination of the test no inhibition of growth rate or yield was observed for test item concentrations up to and including 31.3 mg/L. At the highest test item concentration of 100 mg/L the inhibition of growth rate peaked at 10.8 % and the inhibition of yield peaked at 36.3 %
The measured initial concentrations of the test substance ranged from 94 % to 95 % of nominal. In the aged samples the measured concentrations were between 99 % and 101 % of nominal. Therefore toxicological endpoints were evaluated using the nominal concentrations of the test item. - Results with reference substance (positive control):
- ErC50= 1.6 mg/L (nominal)
- Reported statistics and error estimates:
- Due to an inhibition of all parameters (growth rate, yield) below 50 % the database was weak for probit analysis, hence the EC10- value for growth rate and yield as well as the EC20-value for yield was not determined. The EC20-value for growth rate and EC50-value for growth rate and yield was considered to be > 100 mg/L (nominal).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- After 72 h no inhibition of growth rate or yield was observed for test item concentrations up to and including 31.3 mg/L. Significant inhibitory effects were determined for growth rate and yield at test item concentrations of 100 mg/L (nominal). The overall LOEC was therefore determined to be 100 mg/L (nominal), the corresponding NOEC was set at 31.3 mg/L (nominal).
Due to an inhibition of all parameters (growth rate, yield) below 50 % the database was weak for probit analysis, hence the EC10- value for growth rate and yield as well as the EC20-value for yield was not determined. The EC20-value for growth rate and EC50-value for growth rate and yield was considered to be > 100 mg/L (nominal).
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