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EC number: 273-105-4 | CAS number: 68937-98-4 This substance is identified by SDA Substance Name: C14-C18 and C12-C20 unsaturated alkyl alkene and C14-C18 and C12-C20 unsaturated alkyl hydroxy sulfonic acid sodium salt and SDA Reporting Number: 03-059-04.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 September 2017 - 14 December 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- HPLC-MS/MS
- Details on sampling:
- - Concentrations: 1.0, 3.2, 10, 32 and 100 mg/L in main test
- Sampling method: Samples were taken at 27 hours and the cell densities determined using a Coulter® Multisizer Particle Counter. At 49 and 72 hours, cell densities were determined using a haemocytometer and light microscope due to the presence of a precipitate in the 32 and 100 mg/L test preparations.
- Sample storage conditions before analysis: frozen - Vehicle:
- yes
- Remarks:
- culture medium
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test item dissolved in culture medium
- Controls: 6 flasks used for control without algae
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: CCAP 278/4.
- Source: Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Scottish Marine Institute
- Culturing media and conditions: same as test - Test type:
- not specified
- Water media type:
- other: reverse osmosis purified deionized water
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- not specified
- Post exposure observation period:
- not specified
- Hardness:
- not specified
- Test temperature:
- 24 ±1 ºC
- pH:
- pH 7.9 at 0 hours to pH 8.1 at 72 hours
- Dissolved oxygen:
- not specified
- Salinity:
- not specified
- Conductivity:
- not specified
- Nominal and measured concentrations:
- nominal: 0.1, 1, 10, 100mg/L
measured: 91% to 142% of nominal thereby indicating that the decline in measured concentrations observed was due to adsorption of the test item to the algal cells present rather than instability of the test item under the conditions of the test. As such the results have been calculated based on nominal test concentrations only as it was considered that the algae would have been exposed to nominal concentrations throughout the duration of the test. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250mL conical flask
- Type (delete if not applicable): closed with polyurethane foam bungs to reduce evaporation
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Initial cells density: 5.00 x 10e3 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reverse osmosis purified deionized water
- Culture medium different from test medium: no
- Intervals of water quality measurement: initiation and 72hours
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Photoperiod: continuous
- Light intensity and quality: 7000 lux, warm white lighting (380 to 730 nm)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 27, 49, 72 hours
- Determination of cell concentrations: coulter counter,
At 49 and 72 hours, cell densities were determined using a haemocytometer and light microscope due to the presence of a precipitate in the 32 and 100 mg/L test preparations
TEST CONCENTRATIONS
- Range finding study: 0.10, 1.0, 10 and 100 mg/L
- Test concentrations: 1.0, 3.2, 10, 32 and 100 mg/L.
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 89 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 6.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none
- Unusual cell shape: some enlarged cells were observed
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: At 49 and 72 hours, cell densities were determined using a haemocytometer and light microscope due to the presence of a precipitate in the 32 and 100 mg/L test preparations - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 1.6 mg/L at 72 hour
- Other:
No Observed Effect Concentration based on growth rate: 0.25 mg/L
No Observed Effect Concentration based on yield: 0.25 mg/L
Lowest Observed Effect Concentration based on growth rate: 0.50 mg/L
Lowest Observed Effect Concentration based on yield: 0.50 mg/L - Reported statistics and error estimates:
- not specified
- Validity criteria fulfilled:
- yes
- Conclusions:
- Growth Rate: EC50 = 89mg/L, NOEC =1.0mg/L, LOEC =3.2mg/L
Yield: EC50 = 6.9mg/L, NOEC = 1mg/L, LOEC = 3.2mg/L
Reference
Description of key information
72 hours:
Growth Rate: EC50 = 89mg/L, NOEC =1.0mg/L, LOEC =3.2mg/L
Yield: EC50 = 6.9mg/L, NOEC = 1mg/L, LOEC = 3.2mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 89 mg/L
- EC10 or NOEC for freshwater algae:
- 1 mg/L
Additional information
An algal growth inhibition study was conducted (Envigo Research Limited, 2018) to determine the effect of Sulfonic acids, C14-18-alkane hydroxy and C12-20-alkapolyene and C14-18-alkene and C12-20-alkene hydroxy, sodium salts on the growth of green alga (Pseudokirchneriella subcapitata). The study was conducted according to OECD test guideline 201 and EC method C3, and in compliance with GLP.
Triplicate algal cultures were exposed to Sulfonic acids, C14-18-alkane hydroxy and C12-20-alkapolyene and C14-18-alkene and C12-20-alkene hydroxy, sodium salts at concentrations 1.0, 3.2, 10.0, 32.0 and 100 mg/L and incubated under continuous illumination for 72 hours. Six replicate control cultures were also incubated concurrently with the test samples.
The yield of Biomass was affected by treatment with Sulfonic acids, C14-18-alkane hydroxy and C12-20-alkapolyene and C14-18-alkene and C12-20-alkene hydroxy, sodium salts at all levels, and so the No Observed Effect level in terms of Biomass was less than 1.0 mg/L, the lowest concentration tested. An effect on growth rate was also seen at all levels. The NOEC in terms of growth rate was concluded to be 1.0 mg/L. The EC50 in terms of both growth rate and yield were 89.0 and 6.9 mg/L, respectively.
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